SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES
Level of serum secretory immunoglobulin A was investigated in alcoholic liver disease (ALD, 59 men and 23 women) and non-alcoholic fatty liver disease (NAFLD, 17 men and 93 women).The data were compared with those of persons without liver pathology (control group, 43 men and 73 women). Moreover, we...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-11-01
|
| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/734 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849401440013910016 |
|---|---|
| author | N. V. Mal’tseva O. F. Lykova A. V. Morozova S. V. Arkhipova Ya. A. Gorbatovskii |
| author_facet | N. V. Mal’tseva O. F. Lykova A. V. Morozova S. V. Arkhipova Ya. A. Gorbatovskii |
| author_sort | N. V. Mal’tseva |
| collection | DOAJ |
| description | Level of serum secretory immunoglobulin A was investigated in alcoholic liver disease (ALD, 59 men and 23 women) and non-alcoholic fatty liver disease (NAFLD, 17 men and 93 women).The data were compared with those of persons without liver pathology (control group, 43 men and 73 women). Moreover, we studied possible associations between ssIgA level and Gln223Arg polymorphism of the LEPR gene. Immunological and DNA diagnostics was performed by means of, respectively, ELISA and allele-specific polymerase chain reaction. We have found that the average level of ssIgA was three times higher in ALD group (11.45±0.82 mg/l), than in the NAFLD group (4.35±0.35 mg/l) or in controls (3,60±0,29 mg/l). SsIgА concentration did not depend on adiposity and gender. The ssIgА concentration proved to be increased in Gln223Arg heterozygotes with NAFLD, when compared with controls. However, the frequency of 223Arg and 223Gln alleles was virtually equal in all observed groups with above-normal concentration of ssIgА, as compared to a sub-group with normal ssIgА concentration. Hence, we have not revealed any significant association between Gln223Arg polymorphism of LEPR and ssIgA level. The data obtained will be useful for studying genetic risk factors in development of infectious mucosаl lesions. |
| format | Article |
| id | doaj-art-807c19d7a2564b1ea63ead4e4244c3b0 |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-11-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-807c19d7a2564b1ea63ead4e4244c3b02025-08-20T03:37:46ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-11-0116546547210.15789/1563-0625-2014-5-465-472714SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASESN. V. Mal’tseva0O. F. Lykova1A. V. Morozova2S. V. Arkhipova3Ya. A. Gorbatovskii4Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian FederationNovokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian FederationNovokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian FederationNovokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian FederationNovokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian FederationLevel of serum secretory immunoglobulin A was investigated in alcoholic liver disease (ALD, 59 men and 23 women) and non-alcoholic fatty liver disease (NAFLD, 17 men and 93 women).The data were compared with those of persons without liver pathology (control group, 43 men and 73 women). Moreover, we studied possible associations between ssIgA level and Gln223Arg polymorphism of the LEPR gene. Immunological and DNA diagnostics was performed by means of, respectively, ELISA and allele-specific polymerase chain reaction. We have found that the average level of ssIgA was three times higher in ALD group (11.45±0.82 mg/l), than in the NAFLD group (4.35±0.35 mg/l) or in controls (3,60±0,29 mg/l). SsIgА concentration did not depend on adiposity and gender. The ssIgА concentration proved to be increased in Gln223Arg heterozygotes with NAFLD, when compared with controls. However, the frequency of 223Arg and 223Gln alleles was virtually equal in all observed groups with above-normal concentration of ssIgА, as compared to a sub-group with normal ssIgА concentration. Hence, we have not revealed any significant association between Gln223Arg polymorphism of LEPR and ssIgA level. The data obtained will be useful for studying genetic risk factors in development of infectious mucosаl lesions.https://www.mimmun.ru/mimmun/article/view/734serum secretory igalepr gene polymorphismhepatic disorders |
| spellingShingle | N. V. Mal’tseva O. F. Lykova A. V. Morozova S. V. Arkhipova Ya. A. Gorbatovskii SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES Медицинская иммунология serum secretory iga lepr gene polymorphism hepatic disorders |
| title | SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES |
| title_full | SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES |
| title_fullStr | SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES |
| title_full_unstemmed | SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES |
| title_short | SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES |
| title_sort | serum secretory immunoglobulin a and gln223arg polymorphism of the lepr gene in alcoholic and non alcoholic fatty liver diseases |
| topic | serum secretory iga lepr gene polymorphism hepatic disorders |
| url | https://www.mimmun.ru/mimmun/article/view/734 |
| work_keys_str_mv | AT nvmaltseva serumsecretoryimmunoglobulinaandgln223argpolymorphismoftheleprgeneinalcoholicandnonalcoholicfattyliverdiseases AT oflykova serumsecretoryimmunoglobulinaandgln223argpolymorphismoftheleprgeneinalcoholicandnonalcoholicfattyliverdiseases AT avmorozova serumsecretoryimmunoglobulinaandgln223argpolymorphismoftheleprgeneinalcoholicandnonalcoholicfattyliverdiseases AT svarkhipova serumsecretoryimmunoglobulinaandgln223argpolymorphismoftheleprgeneinalcoholicandnonalcoholicfattyliverdiseases AT yaagorbatovskii serumsecretoryimmunoglobulinaandgln223argpolymorphismoftheleprgeneinalcoholicandnonalcoholicfattyliverdiseases |