Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro Study
Background: Healthcare-associated infections (HAIs) caused by ESKAPE pathogens are a significant concern in tertiary hospitals worldwide, including those in Al-Kharj, Saudi Arabia. This study aimed to explore the bioactive compounds from Valeriana officinalis and evaluate their antimicrobial activit...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1253_24 |
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| _version_ | 1850195339655512064 |
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| author | Mohammed S. Khan MD Nadeem Bari |
| author_facet | Mohammed S. Khan MD Nadeem Bari |
| author_sort | Mohammed S. Khan |
| collection | DOAJ |
| description | Background:
Healthcare-associated infections (HAIs) caused by ESKAPE pathogens are a significant concern in tertiary hospitals worldwide, including those in Al-Kharj, Saudi Arabia. This study aimed to explore the bioactive compounds from Valeriana officinalis and evaluate their antimicrobial activity against ESKAPE pathogens.
Materials and Methods:
Methanolic extracts of the Valeriana officinalis stem were prepared and subjected to phytochemical analysis, thin-layer chromatography (TLC), and antimicrobial testing using the well-diffusion method. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and cytotoxicity (MTT assay) were also evaluated.
Results:
The extract contained several major phytochemicals with significant activity against ESKAPE pathogens. The MIC values were 50 mg/mL for Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa, 100 mg/mL for Enterococcus faecalis and Staphylococcus aureus, and 25 mg/mL for Escherichia coli. The MBC values were 25 mg/mL for Escherichia coli and 100 mg/mL for the other ESKAPE pathogens.
Conclusion:
The methanolic extract of Valeriana officinalis L. demonstrated potent antimicrobial activity, with relatively low MIC and MBC values against ESKAPE pathogens responsible for HAIs. |
| format | Article |
| id | doaj-art-80671799153b41f29e2d8989d5bec2a0 |
| institution | OA Journals |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-80671799153b41f29e2d8989d5bec2a02025-08-20T02:13:47ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 5S4649S465410.4103/jpbs.jpbs_1253_24Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro StudyMohammed S. KhanMD Nadeem BariBackground: Healthcare-associated infections (HAIs) caused by ESKAPE pathogens are a significant concern in tertiary hospitals worldwide, including those in Al-Kharj, Saudi Arabia. This study aimed to explore the bioactive compounds from Valeriana officinalis and evaluate their antimicrobial activity against ESKAPE pathogens. Materials and Methods: Methanolic extracts of the Valeriana officinalis stem were prepared and subjected to phytochemical analysis, thin-layer chromatography (TLC), and antimicrobial testing using the well-diffusion method. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and cytotoxicity (MTT assay) were also evaluated. Results: The extract contained several major phytochemicals with significant activity against ESKAPE pathogens. The MIC values were 50 mg/mL for Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa, 100 mg/mL for Enterococcus faecalis and Staphylococcus aureus, and 25 mg/mL for Escherichia coli. The MBC values were 25 mg/mL for Escherichia coli and 100 mg/mL for the other ESKAPE pathogens. Conclusion: The methanolic extract of Valeriana officinalis L. demonstrated potent antimicrobial activity, with relatively low MIC and MBC values against ESKAPE pathogens responsible for HAIs.https://journals.lww.com/10.4103/jpbs.jpbs_1253_24antibacterial activityantimicrobial resistanceeskape pathogensphytocompoundsvaleriana officinalis l |
| spellingShingle | Mohammed S. Khan MD Nadeem Bari Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro Study Journal of Pharmacy and Bioallied Sciences antibacterial activity antimicrobial resistance eskape pathogens phytocompounds valeriana officinalis l |
| title | Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro Study |
| title_full | Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro Study |
| title_fullStr | Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro Study |
| title_full_unstemmed | Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro Study |
| title_short | Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro Study |
| title_sort | evaluating anti eskape potential of valeriana officinalis l extract against healthcare associated infections in tertiary hospitals of al kharj governorate an in vitro study |
| topic | antibacterial activity antimicrobial resistance eskape pathogens phytocompounds valeriana officinalis l |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_1253_24 |
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