Evaluating Anti-ESKAPE Potential of Valeriana Officinalis L. Extract against Healthcare-Associated Infections in Tertiary Hospitals of Al-Kharj Governorate: An In Vitro Study
Background: Healthcare-associated infections (HAIs) caused by ESKAPE pathogens are a significant concern in tertiary hospitals worldwide, including those in Al-Kharj, Saudi Arabia. This study aimed to explore the bioactive compounds from Valeriana officinalis and evaluate their antimicrobial activit...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1253_24 |
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| Summary: | Background:
Healthcare-associated infections (HAIs) caused by ESKAPE pathogens are a significant concern in tertiary hospitals worldwide, including those in Al-Kharj, Saudi Arabia. This study aimed to explore the bioactive compounds from Valeriana officinalis and evaluate their antimicrobial activity against ESKAPE pathogens.
Materials and Methods:
Methanolic extracts of the Valeriana officinalis stem were prepared and subjected to phytochemical analysis, thin-layer chromatography (TLC), and antimicrobial testing using the well-diffusion method. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and cytotoxicity (MTT assay) were also evaluated.
Results:
The extract contained several major phytochemicals with significant activity against ESKAPE pathogens. The MIC values were 50 mg/mL for Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa, 100 mg/mL for Enterococcus faecalis and Staphylococcus aureus, and 25 mg/mL for Escherichia coli. The MBC values were 25 mg/mL for Escherichia coli and 100 mg/mL for the other ESKAPE pathogens.
Conclusion:
The methanolic extract of Valeriana officinalis L. demonstrated potent antimicrobial activity, with relatively low MIC and MBC values against ESKAPE pathogens responsible for HAIs. |
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| ISSN: | 0976-4879 0975-7406 |