A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20
Abstract Roxarsone (V) (Rox(V)) is an organoarsenical compound that poses significant risks to aquatic ecosystems and various diseases. Reducing trivalent 3-amino-4-hydroxyphenylarsonic acid (HAPA(III)) offers a competitive advantage; however, it leads to localized arsenic contamination, which can d...
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BMC
2025-01-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-024-03740-4 |
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| author | Xuehui Zong Xuyang Wang Minghui Yu Jiahui Wang Congcong Li Bing Wang Yongan Wang |
| author_facet | Xuehui Zong Xuyang Wang Minghui Yu Jiahui Wang Congcong Li Bing Wang Yongan Wang |
| author_sort | Xuehui Zong |
| collection | DOAJ |
| description | Abstract Roxarsone (V) (Rox(V)) is an organoarsenical compound that poses significant risks to aquatic ecosystems and various diseases. Reducing trivalent 3-amino-4-hydroxyphenylarsonic acid (HAPA(III)) offers a competitive advantage; however, it leads to localized arsenic contamination, which can disrupt the soil microbiome and impede plant growth. Three genes, BsntrA, arsC2, and BsexpA, encoding nitroreductase, arsenate reductase, and MFS transporter, respectively, were identified in the Rox(V)-resistant strain Brevundimonas sp. M20. A three-step approach, including nitroreduction, As(V) reduction, and HAPA(III) secretion, which is responsible for roxarsone(V) resistance, was subsequently confirmed. Moreover, the flavonoid compound baicalin occupied the HAPA(III) delivery space and grabbed the R127 residues via stronger interactions. This steric hindrance prevented the transportation of HAPA(III) by BsexpA to the extracellular space. These results demonstrate a new Rox(V) reduction pathway, providing a potential efflux pump inhibitor to trap more toxins. |
| format | Article |
| id | doaj-art-8057aaa413b64ef99176bb7834e15d05 |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj-art-8057aaa413b64ef99176bb7834e15d052025-01-19T12:12:29ZengBMCBMC Microbiology1471-21802025-01-0125111210.1186/s12866-024-03740-4A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20Xuehui Zong0Xuyang Wang1Minghui Yu2Jiahui Wang3Congcong Li4Bing Wang5Yongan Wang6School of Laboratory Animal & Shandong Laboratory Animal Center, Shandong First Medical University, Shandong Academy of Medical SciencesBiomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical SciencesBiomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical SciencesBiomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical SciencesShandong Quancheng Test & Technology Limited CompanyShandong Quancheng Test & Technology Limited CompanySchool of Laboratory Animal & Shandong Laboratory Animal Center, Shandong First Medical University, Shandong Academy of Medical SciencesAbstract Roxarsone (V) (Rox(V)) is an organoarsenical compound that poses significant risks to aquatic ecosystems and various diseases. Reducing trivalent 3-amino-4-hydroxyphenylarsonic acid (HAPA(III)) offers a competitive advantage; however, it leads to localized arsenic contamination, which can disrupt the soil microbiome and impede plant growth. Three genes, BsntrA, arsC2, and BsexpA, encoding nitroreductase, arsenate reductase, and MFS transporter, respectively, were identified in the Rox(V)-resistant strain Brevundimonas sp. M20. A three-step approach, including nitroreduction, As(V) reduction, and HAPA(III) secretion, which is responsible for roxarsone(V) resistance, was subsequently confirmed. Moreover, the flavonoid compound baicalin occupied the HAPA(III) delivery space and grabbed the R127 residues via stronger interactions. This steric hindrance prevented the transportation of HAPA(III) by BsexpA to the extracellular space. These results demonstrate a new Rox(V) reduction pathway, providing a potential efflux pump inhibitor to trap more toxins.https://doi.org/10.1186/s12866-024-03740-4Roxarsone (V)NitroreductaseArsenate reductaseMFS efflux pumpBaicalinEfflux pump inhibitor |
| spellingShingle | Xuehui Zong Xuyang Wang Minghui Yu Jiahui Wang Congcong Li Bing Wang Yongan Wang A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20 BMC Microbiology Roxarsone (V) Nitroreductase Arsenate reductase MFS efflux pump Baicalin Efflux pump inhibitor |
| title | A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20 |
| title_full | A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20 |
| title_fullStr | A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20 |
| title_full_unstemmed | A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20 |
| title_short | A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20 |
| title_sort | reduction secretion system contributes to roxarsone v degradation and efflux in brevundimonas sp m20 |
| topic | Roxarsone (V) Nitroreductase Arsenate reductase MFS efflux pump Baicalin Efflux pump inhibitor |
| url | https://doi.org/10.1186/s12866-024-03740-4 |
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