Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencing
Summary: Gene deletions perturb cellular homeostasis, affecting gene expression and phenotypes. Here, we present a protocol for serial transfer experiments in Saccharomyces cerevisiae strains following targeted gene knockouts. We describe steps for gene deletion, serial passage optimization, and gro...
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002631 |
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| author | Bei Jiang Chuyao Xiao Li Liu |
| author_facet | Bei Jiang Chuyao Xiao Li Liu |
| author_sort | Bei Jiang |
| collection | DOAJ |
| description | Summary: Gene deletions perturb cellular homeostasis, affecting gene expression and phenotypes. Here, we present a protocol for serial transfer experiments in Saccharomyces cerevisiae strains following targeted gene knockouts. We describe steps for gene deletion, serial passage optimization, and growth kinetics analysis. We then detail procedures for genomic and transcriptomic profiling. This protocol enables the investigation of changes in yeast strains resulting from gene loss.For complete details on the use and execution of this protocol, please refer to Jiang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-804c54f567df48b2bf8ce92d398d0fe7 |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-804c54f567df48b2bf8ce92d398d0fe72025-08-20T02:04:44ZengElsevierSTAR Protocols2666-16672025-06-016210385710.1016/j.xpro.2025.103857Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencingBei Jiang0Chuyao Xiao1Li Liu2MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; Greater Bay Area Institute of Precision Medicine (Guangzhou), Fudan University, Nansha District, Guangzhou 511400, China; Institute of Life Sciences, Fudan University, Shanghai 200433, ChinaMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; Corresponding authorSummary: Gene deletions perturb cellular homeostasis, affecting gene expression and phenotypes. Here, we present a protocol for serial transfer experiments in Saccharomyces cerevisiae strains following targeted gene knockouts. We describe steps for gene deletion, serial passage optimization, and growth kinetics analysis. We then detail procedures for genomic and transcriptomic profiling. This protocol enables the investigation of changes in yeast strains resulting from gene loss.For complete details on the use and execution of this protocol, please refer to Jiang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002631GeneticsMolecular Biology |
| spellingShingle | Bei Jiang Chuyao Xiao Li Liu Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencing STAR Protocols Genetics Molecular Biology |
| title | Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencing |
| title_full | Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencing |
| title_fullStr | Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencing |
| title_full_unstemmed | Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencing |
| title_short | Protocol for adaptive laboratory evolution of S. cerevisiae by PEG/LiAc transformation and sequencing |
| title_sort | protocol for adaptive laboratory evolution of s cerevisiae by peg liac transformation and sequencing |
| topic | Genetics Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002631 |
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