On the antifungal potentials of Piper betle L. ethanol extract against sclerotium rolfsii for pesticidal applicability: In vitro evidence and in silico screening

Piper betle L. is ubiquitous and known by folk experiences for its antimicrobial properties, thus promising as a low-cost green pesticide. In this work, the plant ethanol extract was subjected to a methodical theory-experiment investigation against Sclerotium rolfsii. The extract composition was spe...

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Main Authors: Nguyen Thi Thanh Hai, Thanh Q. Bui, Tran Thi Ai My, Nguyen Thi Thu Thuy, Nguyen Vinh Phu, Phan Tu Quy, Dao Cuong To, Tran Nhat Phong Dao, Sunday Amos Onikanni, Ho Viet Duc, Nguyen Chi Bao, Nguyen Thi Ai Nhung
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Results in Chemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S2211715625004606
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author Nguyen Thi Thanh Hai
Thanh Q. Bui
Tran Thi Ai My
Nguyen Thi Thu Thuy
Nguyen Vinh Phu
Phan Tu Quy
Dao Cuong To
Tran Nhat Phong Dao
Sunday Amos Onikanni
Ho Viet Duc
Nguyen Chi Bao
Nguyen Thi Ai Nhung
author_facet Nguyen Thi Thanh Hai
Thanh Q. Bui
Tran Thi Ai My
Nguyen Thi Thu Thuy
Nguyen Vinh Phu
Phan Tu Quy
Dao Cuong To
Tran Nhat Phong Dao
Sunday Amos Onikanni
Ho Viet Duc
Nguyen Chi Bao
Nguyen Thi Ai Nhung
author_sort Nguyen Thi Thanh Hai
collection DOAJ
description Piper betle L. is ubiquitous and known by folk experiences for its antimicrobial properties, thus promising as a low-cost green pesticide. In this work, the plant ethanol extract was subjected to a methodical theory-experiment investigation against Sclerotium rolfsii. The extract composition was spectroscopically characterized with 40 components; in which, 17 candidates (1–17; notated aligning with content ranks) were opted for computer-based studies. The antifungal assays evidenced the bioactive potential of the total extracts against S. rolfsii (full inhibition effect after 7 days; all concentrations). The inhibitability was predicted by docking simulation, i.e.: 5-2OFC (DS¯ -10.9 kcal.mol−1; DSmax-12.9 kcal.mol−1) > 12-2OFC (DS¯ -9.9 kcal.mol−1; DSmax-12.1 kcal.mol−1) > 7-2OFC (DS¯ -9.7 kcal.mol−1; DSmax-12.2 kcal.mol−1) ≈ 17-2OFC (DS¯ -9.6 kcal.mol−1; DSmax-12.7 kcal.mol−1) > 1-2OFC (DS¯ -9.4 kcal.mol−1; DSmax-12.0 kcal.mol−1). The chemical potentiality was derived from quantum calculation, i.e.: 12 (ground-state energy −1122.93 a.u.; dipole moment 4.30 Debye) and 17 (ground-state energy −1047.98 a.u.; dipole moment 2.84 Debye). The suitability for biological and pesticidal applications was justified by physicochemical analyses. Together, the experimental findings evidence the biological activity and suggest the use of 1 (Benzoic acid, 3,5-dimethyl) given by its highest proportion; the computational retrievals propose further attempts to allocate the primary bio-active agent and predict 12 (Linolenic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (Z,Z,Z)) given by its favorable properties.
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spelling doaj-art-8047a6ee592847d0970cfa4ee4b752b92025-08-20T03:02:26ZengElsevierResults in Chemistry2211-71562025-07-011610247710.1016/j.rechem.2025.102477On the antifungal potentials of Piper betle L. ethanol extract against sclerotium rolfsii for pesticidal applicability: In vitro evidence and in silico screeningNguyen Thi Thanh Hai0Thanh Q. Bui1Tran Thi Ai My2Nguyen Thi Thu Thuy3Nguyen Vinh Phu4Phan Tu Quy5Dao Cuong To6Tran Nhat Phong Dao7Sunday Amos Onikanni8Ho Viet Duc9Nguyen Chi Bao10Nguyen Thi Ai Nhung11Department of Chemistry, University of Sciences, Hue University, Hue 49000, Viet NamDepartment of Chemistry, University of Sciences, Hue University, Hue 49000, Viet NamDepartment of Chemistry, University of Sciences, Hue University, Hue 49000, Viet NamFaculty of Agronomy, University of Agriculture and Forestry, Hue University, Hue 49000, Viet NamFaculty of Basic Sciences, University of Medicine and Pharmacy, Hue University, Hue 49000, Viet NamDepartment of Natural Sciences & Technology, Tay Nguyen University, Buon Ma Thuot 630000, Viet NamPhenikaa University Nano Institute (PHENA), Phenikaa University, Yen Nghia, Ha Dong, Hanoi 12116, Viet NamFaculty of Traditional Medicine, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Viet NamLaboratory of Experimental Endocrinology–LEEx, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil; Department of Chemical Sciences, Biochemistry Unit, Afe-Babalola University, Ado-Ekiti, Ekiti State, NigeriaDepartment of Pharmacy, Hue university of Medicine and Pharmacy, Hue University, Hue 49000, Viet NamHue University, Hue 49000, Viet NamDepartment of Chemistry, University of Sciences, Hue University, Hue 49000, Viet Nam; Corresponding author.Piper betle L. is ubiquitous and known by folk experiences for its antimicrobial properties, thus promising as a low-cost green pesticide. In this work, the plant ethanol extract was subjected to a methodical theory-experiment investigation against Sclerotium rolfsii. The extract composition was spectroscopically characterized with 40 components; in which, 17 candidates (1–17; notated aligning with content ranks) were opted for computer-based studies. The antifungal assays evidenced the bioactive potential of the total extracts against S. rolfsii (full inhibition effect after 7 days; all concentrations). The inhibitability was predicted by docking simulation, i.e.: 5-2OFC (DS¯ -10.9 kcal.mol−1; DSmax-12.9 kcal.mol−1) > 12-2OFC (DS¯ -9.9 kcal.mol−1; DSmax-12.1 kcal.mol−1) > 7-2OFC (DS¯ -9.7 kcal.mol−1; DSmax-12.2 kcal.mol−1) ≈ 17-2OFC (DS¯ -9.6 kcal.mol−1; DSmax-12.7 kcal.mol−1) > 1-2OFC (DS¯ -9.4 kcal.mol−1; DSmax-12.0 kcal.mol−1). The chemical potentiality was derived from quantum calculation, i.e.: 12 (ground-state energy −1122.93 a.u.; dipole moment 4.30 Debye) and 17 (ground-state energy −1047.98 a.u.; dipole moment 2.84 Debye). The suitability for biological and pesticidal applications was justified by physicochemical analyses. Together, the experimental findings evidence the biological activity and suggest the use of 1 (Benzoic acid, 3,5-dimethyl) given by its highest proportion; the computational retrievals propose further attempts to allocate the primary bio-active agent and predict 12 (Linolenic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (Z,Z,Z)) given by its favorable properties.http://www.sciencedirect.com/science/article/pii/S2211715625004606Antifungal activityPiper betle L.Sclerotium rolfsii
spellingShingle Nguyen Thi Thanh Hai
Thanh Q. Bui
Tran Thi Ai My
Nguyen Thi Thu Thuy
Nguyen Vinh Phu
Phan Tu Quy
Dao Cuong To
Tran Nhat Phong Dao
Sunday Amos Onikanni
Ho Viet Duc
Nguyen Chi Bao
Nguyen Thi Ai Nhung
On the antifungal potentials of Piper betle L. ethanol extract against sclerotium rolfsii for pesticidal applicability: In vitro evidence and in silico screening
Results in Chemistry
Antifungal activity
Piper betle L.
Sclerotium rolfsii
title On the antifungal potentials of Piper betle L. ethanol extract against sclerotium rolfsii for pesticidal applicability: In vitro evidence and in silico screening
title_full On the antifungal potentials of Piper betle L. ethanol extract against sclerotium rolfsii for pesticidal applicability: In vitro evidence and in silico screening
title_fullStr On the antifungal potentials of Piper betle L. ethanol extract against sclerotium rolfsii for pesticidal applicability: In vitro evidence and in silico screening
title_full_unstemmed On the antifungal potentials of Piper betle L. ethanol extract against sclerotium rolfsii for pesticidal applicability: In vitro evidence and in silico screening
title_short On the antifungal potentials of Piper betle L. ethanol extract against sclerotium rolfsii for pesticidal applicability: In vitro evidence and in silico screening
title_sort on the antifungal potentials of piper betle l ethanol extract against sclerotium rolfsii for pesticidal applicability in vitro evidence and in silico screening
topic Antifungal activity
Piper betle L.
Sclerotium rolfsii
url http://www.sciencedirect.com/science/article/pii/S2211715625004606
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