FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPID
Propionibacterium acne, Bacillus cereus and Escherichia coli are bacteria that cause infections of the skin and digestive tract in humans. Sargassum duplicatum, one of brown macroalgae species, is known to have the antibacterial activities. This study aims to prove antibacterial activities of S....
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2022-03-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202201&vol=1&aid=5389 |
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| author | RIONG S. PANJAITAN ARI T. W. ASTUTI HILWA R. NOVITA FUAD ALHADI |
| author_facet | RIONG S. PANJAITAN ARI T. W. ASTUTI HILWA R. NOVITA FUAD ALHADI |
| author_sort | RIONG S. PANJAITAN |
| collection | DOAJ |
| description | Propionibacterium acne, Bacillus cereus and Escherichia
coli are bacteria that cause infections of the skin and digestive tract in
humans. Sargassum duplicatum, one of brown macroalgae species, is
known to have the antibacterial activities. This study aims to prove
antibacterial activities of S. duplicatum lipid against three different bacteria.
The method used is the Folch method (using soxhletation, with a ratio of
chloroform: methanol 2:1, v/v) to extract lipid. Furthermore, fatty acids
content was identified by gas chromatography - mass spectrophotometry
(GC-MS). Then, the disc diffusion method was used to measure the
inhibition zone for antibacterial activity by using tetracycline as positive
control. The lipid percentage of S. duplicatum was obtained about 2.502 %
(v/w) where there were two lipid layers namely chloroform layer (0.388 %
(v/w)) and methanol layer (2.114 % (v/w)). S. duplicatum lipid contained
palmitic acid (in both layers) and oleic acid (only methanol layer).
Moreover, methanol and chloroform lipid layer (1000 mg·mL-1) of S.
duplicatum showed antibacterial activity against B. cereus, E. coli and P.
acne |
| format | Article |
| id | doaj-art-80421bc7b9224dfe970dcf3956980e6f |
| institution | OA Journals |
| issn | 1582-540X |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-80421bc7b9224dfe970dcf3956980e6f2025-08-20T01:57:56ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X2022-03-01231021029FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPIDRIONG S. PANJAITAN0ARI T. W. ASTUTI1HILWA R. NOVITA2FUAD ALHADI3Universitas 17 Agustus 1945 Jakarta, Faculty of Pharmacy, 14350, North Jakarta, IndonesiaUniversitas 17 Agustus 1945 Jakarta, Faculty of Pharmacy, 14350, North Jakarta, IndonesiaUniversitas 17 Agustus 1945 Jakarta, Faculty of Pharmacy, 14350, North Jakarta, IndonesiaUniversitas 17 Agustus 1945 Jakarta, Faculty of Pharmacy, 14350, North Jakarta, IndonesiaPropionibacterium acne, Bacillus cereus and Escherichia coli are bacteria that cause infections of the skin and digestive tract in humans. Sargassum duplicatum, one of brown macroalgae species, is known to have the antibacterial activities. This study aims to prove antibacterial activities of S. duplicatum lipid against three different bacteria. The method used is the Folch method (using soxhletation, with a ratio of chloroform: methanol 2:1, v/v) to extract lipid. Furthermore, fatty acids content was identified by gas chromatography - mass spectrophotometry (GC-MS). Then, the disc diffusion method was used to measure the inhibition zone for antibacterial activity by using tetracycline as positive control. The lipid percentage of S. duplicatum was obtained about 2.502 % (v/w) where there were two lipid layers namely chloroform layer (0.388 % (v/w)) and methanol layer (2.114 % (v/w)). S. duplicatum lipid contained palmitic acid (in both layers) and oleic acid (only methanol layer). Moreover, methanol and chloroform lipid layer (1000 mg·mL-1) of S. duplicatum showed antibacterial activity against B. cereus, E. coli and P. acnehttps://pubs.ub.ro/?pg=revues&rev=cscc6&num=202201&vol=1&aid=5389disc diffusionextractionfolch methodoleic acidpalmitic acid |
| spellingShingle | RIONG S. PANJAITAN ARI T. W. ASTUTI HILWA R. NOVITA FUAD ALHADI FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPID Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry disc diffusion extraction folch method oleic acid palmitic acid |
| title | FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPID |
| title_full | FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPID |
| title_fullStr | FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPID |
| title_full_unstemmed | FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPID |
| title_short | FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPID |
| title_sort | fatty acids content and antibacterial activity of sargassum duplicatum lipid |
| topic | disc diffusion extraction folch method oleic acid palmitic acid |
| url | https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202201&vol=1&aid=5389 |
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