Genomics of cellular proliferation in periodic environmental fluctuations
Abstract Living systems control cell growth dynamically by processing information from their environment. Although responses to a single environmental change have been intensively studied, little is known about how cells react to fluctuating conditions. Here, we address this question at the genomic...
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| Format: | Article |
| Language: | English |
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Springer Nature
2018-03-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.20177823 |
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| author | Jérôme Salignon Magali Richard Etienne Fulcrand Hélène Duplus‐Bottin Gaël Yvert |
| author_facet | Jérôme Salignon Magali Richard Etienne Fulcrand Hélène Duplus‐Bottin Gaël Yvert |
| author_sort | Jérôme Salignon |
| collection | DOAJ |
| description | Abstract Living systems control cell growth dynamically by processing information from their environment. Although responses to a single environmental change have been intensively studied, little is known about how cells react to fluctuating conditions. Here, we address this question at the genomic scale by measuring the relative proliferation rate (fitness) of 3,568 yeast gene deletion mutants in out‐of‐equilibrium conditions: periodic oscillations between two environmental conditions. In periodic salt stress, fitness and its genetic variance largely depended on the oscillating period. Surprisingly, dozens of mutants displayed pronounced hyperproliferation under short stress periods, revealing unexpected controllers of growth under fast dynamics. We validated the implication of the high‐affinity cAMP phosphodiesterase and of a regulator of protein translocation to mitochondria in this group. Periodic oscillations of extracellular methionine, a factor unrelated to salinity, also altered fitness but to a lesser extent and for different genes. The results illustrate how natural selection acts on mutations in a dynamic environment, highlighting unsuspected genetic vulnerabilities to periodic stress in molecular processes that are conserved across all eukaryotes. |
| format | Article |
| id | doaj-art-803a2b4c90ff4be58f32a13fc507b618 |
| institution | DOAJ |
| issn | 1744-4292 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-803a2b4c90ff4be58f32a13fc507b6182025-08-20T03:06:30ZengSpringer NatureMolecular Systems Biology1744-42922018-03-0114311710.15252/msb.20177823Genomics of cellular proliferation in periodic environmental fluctuationsJérôme Salignon0Magali Richard1Etienne Fulcrand2Hélène Duplus‐Bottin3Gaël Yvert4Laboratory of Biology and Modeling of the Cell, Ecole Normale Supérieure de Lyon, CNRS, Université Claude Bernard de Lyon, Université de LyonLaboratory of Biology and Modeling of the Cell, Ecole Normale Supérieure de Lyon, CNRS, Université Claude Bernard de Lyon, Université de LyonLaboratory of Biology and Modeling of the Cell, Ecole Normale Supérieure de Lyon, CNRS, Université Claude Bernard de Lyon, Université de LyonLaboratory of Biology and Modeling of the Cell, Ecole Normale Supérieure de Lyon, CNRS, Université Claude Bernard de Lyon, Université de LyonLaboratory of Biology and Modeling of the Cell, Ecole Normale Supérieure de Lyon, CNRS, Université Claude Bernard de Lyon, Université de LyonAbstract Living systems control cell growth dynamically by processing information from their environment. Although responses to a single environmental change have been intensively studied, little is known about how cells react to fluctuating conditions. Here, we address this question at the genomic scale by measuring the relative proliferation rate (fitness) of 3,568 yeast gene deletion mutants in out‐of‐equilibrium conditions: periodic oscillations between two environmental conditions. In periodic salt stress, fitness and its genetic variance largely depended on the oscillating period. Surprisingly, dozens of mutants displayed pronounced hyperproliferation under short stress periods, revealing unexpected controllers of growth under fast dynamics. We validated the implication of the high‐affinity cAMP phosphodiesterase and of a regulator of protein translocation to mitochondria in this group. Periodic oscillations of extracellular methionine, a factor unrelated to salinity, also altered fitness but to a lesser extent and for different genes. The results illustrate how natural selection acts on mutations in a dynamic environment, highlighting unsuspected genetic vulnerabilities to periodic stress in molecular processes that are conserved across all eukaryotes.https://doi.org/10.15252/msb.20177823fitnessfluctuating environmentselectionstressyeast |
| spellingShingle | Jérôme Salignon Magali Richard Etienne Fulcrand Hélène Duplus‐Bottin Gaël Yvert Genomics of cellular proliferation in periodic environmental fluctuations Molecular Systems Biology fitness fluctuating environment selection stress yeast |
| title | Genomics of cellular proliferation in periodic environmental fluctuations |
| title_full | Genomics of cellular proliferation in periodic environmental fluctuations |
| title_fullStr | Genomics of cellular proliferation in periodic environmental fluctuations |
| title_full_unstemmed | Genomics of cellular proliferation in periodic environmental fluctuations |
| title_short | Genomics of cellular proliferation in periodic environmental fluctuations |
| title_sort | genomics of cellular proliferation in periodic environmental fluctuations |
| topic | fitness fluctuating environment selection stress yeast |
| url | https://doi.org/10.15252/msb.20177823 |
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