Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in Turkey
The aim of the present study was the determination of the prevalence and antibiotic resistance of L. monocytogenes in ready-to-eat (RTE) foods in Ankara, Turkey. In order to detect and isolate L. monocytogenes from 201 RTE food samples, the EN ISO 11290:1 method was used. All isolates were identifie...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2018/7693782 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849410110727651328 |
|---|---|
| author | Pınar Şanlıbaba Başar Uymaz Tezel Gürcü Aybige Çakmak |
| author_facet | Pınar Şanlıbaba Başar Uymaz Tezel Gürcü Aybige Çakmak |
| author_sort | Pınar Şanlıbaba |
| collection | DOAJ |
| description | The aim of the present study was the determination of the prevalence and antibiotic resistance of L. monocytogenes in ready-to-eat (RTE) foods in Ankara, Turkey. In order to detect and isolate L. monocytogenes from 201 RTE food samples, the EN ISO 11290:1 method was used. All isolates were identified using the polymerase chain reaction. The strains were also confirmed by the detection of the hemolysin gene (hlyA). The overall prevalence of L. monocytogenes was 8.5% among the food samples. Seventeen L. monocytogenes strains were examined by the disk diffusion assay for their resistance to 23 antibiotics. All strains were susceptible to erythromycin, clarithromycin, streptomycin, gentamicin, vancomycin, imipenem, trimethoprim, and chloramphenicol, while all strains were resistant to nalidixic acid, ampicillin, penicillin G, linezolid, and clindamycin. The higher resistance was found against oxacillin (94.1%), kanamycin (76.5%), levofloxacin (70.6%), and teicoplanin (64.7%), followed by amoxicillin/clavulanic acid (53.0%), rifampicin (47.1%), and ciprofloxacin (35.3%). A lower incidence of resistance was observed against tetracycline (5.9%), meropenem (5.9%), and trimethoprim/sulfamethoxazole (17.7%). All isolates were multidrug resistant showing resistance to at least three antibiotic classes. High L. monocytogenes prevalence among analyzed RTE foods represents a high risk for public health. Our findings show a high prevalence of L. monocytogenes in RTE foods in Turkey. More effective control strategies for L. monocytogenes are needed to reduce both prevalence and resistance of L. monocytogenes in Turkish RTE foods. |
| format | Article |
| id | doaj-art-8038acb586bc4e899f405f8f11b1aadb |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-8038acb586bc4e899f405f8f11b1aadb2025-08-20T03:35:15ZengWileyJournal of Food Quality0146-94281745-45572018-01-01201810.1155/2018/76937827693782Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in TurkeyPınar Şanlıbaba0Başar Uymaz Tezel1Gürcü Aybige Çakmak2Department of Food Engineering, Engineering Faculty, Ankara University, 50th Year Settlement, 06830 Gölbaşı, Ankara, TurkeyÇanakkale University, Bayramiç Vocational School, Food Technology Program, 17700 Bayramiç, Çanakkale, TurkeyAkdeniz University, Department of Food Engineering, Faculty of Engineering, 07058 Antalya, TurkeyThe aim of the present study was the determination of the prevalence and antibiotic resistance of L. monocytogenes in ready-to-eat (RTE) foods in Ankara, Turkey. In order to detect and isolate L. monocytogenes from 201 RTE food samples, the EN ISO 11290:1 method was used. All isolates were identified using the polymerase chain reaction. The strains were also confirmed by the detection of the hemolysin gene (hlyA). The overall prevalence of L. monocytogenes was 8.5% among the food samples. Seventeen L. monocytogenes strains were examined by the disk diffusion assay for their resistance to 23 antibiotics. All strains were susceptible to erythromycin, clarithromycin, streptomycin, gentamicin, vancomycin, imipenem, trimethoprim, and chloramphenicol, while all strains were resistant to nalidixic acid, ampicillin, penicillin G, linezolid, and clindamycin. The higher resistance was found against oxacillin (94.1%), kanamycin (76.5%), levofloxacin (70.6%), and teicoplanin (64.7%), followed by amoxicillin/clavulanic acid (53.0%), rifampicin (47.1%), and ciprofloxacin (35.3%). A lower incidence of resistance was observed against tetracycline (5.9%), meropenem (5.9%), and trimethoprim/sulfamethoxazole (17.7%). All isolates were multidrug resistant showing resistance to at least three antibiotic classes. High L. monocytogenes prevalence among analyzed RTE foods represents a high risk for public health. Our findings show a high prevalence of L. monocytogenes in RTE foods in Turkey. More effective control strategies for L. monocytogenes are needed to reduce both prevalence and resistance of L. monocytogenes in Turkish RTE foods.http://dx.doi.org/10.1155/2018/7693782 |
| spellingShingle | Pınar Şanlıbaba Başar Uymaz Tezel Gürcü Aybige Çakmak Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in Turkey Journal of Food Quality |
| title | Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in Turkey |
| title_full | Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in Turkey |
| title_fullStr | Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in Turkey |
| title_full_unstemmed | Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in Turkey |
| title_short | Prevalence and Antibiotic Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Foods in Turkey |
| title_sort | prevalence and antibiotic resistance of listeria monocytogenes isolated from ready to eat foods in turkey |
| url | http://dx.doi.org/10.1155/2018/7693782 |
| work_keys_str_mv | AT pınarsanlıbaba prevalenceandantibioticresistanceoflisteriamonocytogenesisolatedfromreadytoeatfoodsinturkey AT basaruymaztezel prevalenceandantibioticresistanceoflisteriamonocytogenesisolatedfromreadytoeatfoodsinturkey AT gurcuaybigecakmak prevalenceandantibioticresistanceoflisteriamonocytogenesisolatedfromreadytoeatfoodsinturkey |