INTERACTION OF DOXORUBICIN WITH DNA-HMG1 COMPLEX
In this study, the interaction of the anthracycline antibiotic doxorubicin with DNA-HMG 1 complex was investigated employing UV/VIS spectroscopy, thermal denaturation and DNA cellulose chromatography techniques. The results indicated that the binding of doxorubicin to the protein reduces its Tm in a...
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| Format: | Article |
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| Language: | English |
| Published: |
University of Tehran
1995-08-01
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| Series: | Journal of Sciences, Islamic Republic of Iran |
| Online Access: | https://jsciences.ut.ac.ir/article_31334_d8baabdbd0ee99b0d7fa5b84008f7955.pdf |
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| Summary: | In this study, the interaction of the anthracycline antibiotic doxorubicin
with DNA-HMG 1 complex was investigated employing UV/VIS spectroscopy,
thermal denaturation and DNA cellulose chromatography techniques. The
results indicated that the binding of doxorubicin to the protein reduces its Tm
in a dose dependent manner. Although doxorubicin protects free DNA
against thermal denaturation in the presence of HMG 1, thermal melts represent
biphasic profile corresponding to DNA- drug, HMG1- drug and free
components. Interaction of antibiotic with DNA - HMGl complex using
DNA cellulose columns also implies that the drug binds strongly to singlestranded
DNA-HMG 1 complex rather than to double- stranded DNA-HMG 1
columns. The results suggest destabilization of HMGl protein by drug action
thereby preventing DNA-HMG 1 complex formation |
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| ISSN: | 1016-1104 2345-6914 |