Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster Generation

Background. The immunohistochemical detection of aldosterone synthase (CYP11B2) and steroid 11β-hydroxylase (CYP11B1) has enabled the identification of aldosterone-producing cell clusters (APCCs) in the subcapsular portion of the human adult adrenal cortex. We hypothesized that adrenals have layered...

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Main Authors: Koshiro Nishimoto, Tsugio Seki, Yuichiro Hayashi, Shuji Mikami, Ghaith Al-Eyd, Ken Nakagawa, Shinya Morita, Takeo Kosaka, Mototsugu Oya, Fumiko Mitani, Makoto Suematsu, Yasuaki Kabe, Kuniaki Mukai
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:International Journal of Endocrinology
Online Access:http://dx.doi.org/10.1155/2016/7834356
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author Koshiro Nishimoto
Tsugio Seki
Yuichiro Hayashi
Shuji Mikami
Ghaith Al-Eyd
Ken Nakagawa
Shinya Morita
Takeo Kosaka
Mototsugu Oya
Fumiko Mitani
Makoto Suematsu
Yasuaki Kabe
Kuniaki Mukai
author_facet Koshiro Nishimoto
Tsugio Seki
Yuichiro Hayashi
Shuji Mikami
Ghaith Al-Eyd
Ken Nakagawa
Shinya Morita
Takeo Kosaka
Mototsugu Oya
Fumiko Mitani
Makoto Suematsu
Yasuaki Kabe
Kuniaki Mukai
author_sort Koshiro Nishimoto
collection DOAJ
description Background. The immunohistochemical detection of aldosterone synthase (CYP11B2) and steroid 11β-hydroxylase (CYP11B1) has enabled the identification of aldosterone-producing cell clusters (APCCs) in the subcapsular portion of the human adult adrenal cortex. We hypothesized that adrenals have layered zonation in early postnatal stages and are remodeled to possess APCCs over time. Purposes. To investigate changes in human adrenocortical zonation with age. Methods. We retrospectively analyzed adrenal tissues prepared from 33 autopsied patients aged between 0 and 50 years. They were immunostained for CYP11B2 and CYP11B1. The percentage of APCC areas over the whole adrenal area (AA/WAA, %) and the number of APCCs (NOA, APCCs/mm2) were calculated by four examiners. Average values were used in statistical analyses. Results. Adrenals under 11 years old had layered zona glomerulosa (ZG) and zona fasciculata (ZF) without apparent APCCs. Some adrenals had an unstained (CYP11B2/CYP11B1-negative) layer between ZG and ZF, resembling the rat undifferentiated cell zone. Average AA/WAA and NOA correlated with age, suggesting that APCC development is associated with aging. Possible APCC-to-APA transitional lesions were incidentally identified in two adult adrenals. Conclusions. The adrenal cortex with layered zonation remodels to possess APCCs over time. APCC generation may be associated with hypertension in adults.
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spelling doaj-art-80206167bc0240bc84b7f3ff3780fdd22025-08-20T02:08:38ZengWileyInternational Journal of Endocrinology1687-83371687-83452016-01-01201610.1155/2016/78343567834356Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster GenerationKoshiro Nishimoto0Tsugio Seki1Yuichiro Hayashi2Shuji Mikami3Ghaith Al-Eyd4Ken Nakagawa5Shinya Morita6Takeo Kosaka7Mototsugu Oya8Fumiko Mitani9Makoto Suematsu10Yasuaki Kabe11Kuniaki Mukai12Department of Uro-Oncology, Saitama Medical University International Medical Center, Hidaka, JapanDepartment of Medical Education, School of Medicine, California University of Science and Medicine, Colton, CA, USADivision of Diagnostic Pathology, Keio University School of Medicine, Shinjuku-ku, JapanDivision of Diagnostic Pathology, Keio University School of Medicine, Shinjuku-ku, JapanDepartment of Medical Education, School of Medicine, California University of Science and Medicine, Colton, CA, USADepartment of Urology, Ichikawa General Hospital, Tokyo Dental College, Ichikawa, JapanDepartment of Urology, Keio University School of Medicine, Shinjuku-ku, JapanDepartment of Urology, Keio University School of Medicine, Shinjuku-ku, JapanDepartment of Urology, Keio University School of Medicine, Shinjuku-ku, JapanDepartment of Biochemistry, Keio University School of Medicine, Shinjuku-ku, JapanDepartment of Biochemistry, Keio University School of Medicine, Shinjuku-ku, JapanDepartment of Biochemistry, Keio University School of Medicine, Shinjuku-ku, JapanDepartment of Biochemistry, Keio University School of Medicine, Shinjuku-ku, JapanBackground. The immunohistochemical detection of aldosterone synthase (CYP11B2) and steroid 11β-hydroxylase (CYP11B1) has enabled the identification of aldosterone-producing cell clusters (APCCs) in the subcapsular portion of the human adult adrenal cortex. We hypothesized that adrenals have layered zonation in early postnatal stages and are remodeled to possess APCCs over time. Purposes. To investigate changes in human adrenocortical zonation with age. Methods. We retrospectively analyzed adrenal tissues prepared from 33 autopsied patients aged between 0 and 50 years. They were immunostained for CYP11B2 and CYP11B1. The percentage of APCC areas over the whole adrenal area (AA/WAA, %) and the number of APCCs (NOA, APCCs/mm2) were calculated by four examiners. Average values were used in statistical analyses. Results. Adrenals under 11 years old had layered zona glomerulosa (ZG) and zona fasciculata (ZF) without apparent APCCs. Some adrenals had an unstained (CYP11B2/CYP11B1-negative) layer between ZG and ZF, resembling the rat undifferentiated cell zone. Average AA/WAA and NOA correlated with age, suggesting that APCC development is associated with aging. Possible APCC-to-APA transitional lesions were incidentally identified in two adult adrenals. Conclusions. The adrenal cortex with layered zonation remodels to possess APCCs over time. APCC generation may be associated with hypertension in adults.http://dx.doi.org/10.1155/2016/7834356
spellingShingle Koshiro Nishimoto
Tsugio Seki
Yuichiro Hayashi
Shuji Mikami
Ghaith Al-Eyd
Ken Nakagawa
Shinya Morita
Takeo Kosaka
Mototsugu Oya
Fumiko Mitani
Makoto Suematsu
Yasuaki Kabe
Kuniaki Mukai
Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster Generation
International Journal of Endocrinology
title Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster Generation
title_full Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster Generation
title_fullStr Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster Generation
title_full_unstemmed Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster Generation
title_short Human Adrenocortical Remodeling Leading to Aldosterone-Producing Cell Cluster Generation
title_sort human adrenocortical remodeling leading to aldosterone producing cell cluster generation
url http://dx.doi.org/10.1155/2016/7834356
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