The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial Cells
Interleukin-1 (IL-1) is an inflammatory mediator that increases Cl- secretion in intestinal epithelial cells. To identify the signal transduction pathway(s) involved in IL-1's action, cells were treated with IL-1 and the levels of cyclooxygenase (COX) enzymes, prostaglandin E2 (PGE2) and phosph...
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| Format: | Article |
| Language: | English |
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Wiley
1999-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359990342 |
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| author | Fadia R. Homaidan Liming Zhao Iman Chakroun Carla A. Martin Robert Burakoff |
| author_facet | Fadia R. Homaidan Liming Zhao Iman Chakroun Carla A. Martin Robert Burakoff |
| author_sort | Fadia R. Homaidan |
| collection | DOAJ |
| description | Interleukin-1 (IL-1) is an inflammatory mediator that increases Cl- secretion in intestinal epithelial cells. To identify the signal transduction pathway(s) involved in IL-1's action, cells were treated with IL-1 and the levels of cyclooxygenase (COX) enzymes, prostaglandin E2 (PGE2) and phospholipase A2-activating protein (PLAP), and the activity of phospholipase A2 (PLA2) were measured. IL-1 caused concentrationand time-dependent increases in the levels of PLA2 activity, and/or in the levels of PLAP, COX-2 and PGE2. The IL-induced increase in PGE2 levels was biphasic, with the first peak due to the increase in PLAP levels, and the second peak due to the increase in COX-2 levels. This increase in PGE2 levels may provide a mechanism for acute and chronic inflammation in the intestine. |
| format | Article |
| id | doaj-art-8013da0051614affa713e2e545d274aa |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8013da0051614affa713e2e545d274aa2025-02-03T05:53:40ZengWileyMediators of Inflammation0962-93511466-18611999-01-0184-518919710.1080/09629359990342The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial CellsFadia R. Homaidan0Liming Zhao1Iman Chakroun2Carla A. Martin3Robert Burakoff4Department of Physiology, Faculty of Medicine, American University of Beirut, Beirut, LebanonDivision of Gastroenterology, Winthrop-University Hospital, Mineola, NY, USADepartment of Physiology, Faculty of Medicine, American University of Beirut, Beirut, LebanonDivision of Gastroenterology, Winthrop-University Hospital, Mineola, NY, USADivision of Gastroenterology, Winthrop-University Hospital, Mineola, NY, USAInterleukin-1 (IL-1) is an inflammatory mediator that increases Cl- secretion in intestinal epithelial cells. To identify the signal transduction pathway(s) involved in IL-1's action, cells were treated with IL-1 and the levels of cyclooxygenase (COX) enzymes, prostaglandin E2 (PGE2) and phospholipase A2-activating protein (PLAP), and the activity of phospholipase A2 (PLA2) were measured. IL-1 caused concentrationand time-dependent increases in the levels of PLA2 activity, and/or in the levels of PLAP, COX-2 and PGE2. The IL-induced increase in PGE2 levels was biphasic, with the first peak due to the increase in PLAP levels, and the second peak due to the increase in COX-2 levels. This increase in PGE2 levels may provide a mechanism for acute and chronic inflammation in the intestine.http://dx.doi.org/10.1080/09629359990342Interleukin-1 receptorCytokinesProstaglandin E2 productionEpithelial cellsInflammatory bowel disease. |
| spellingShingle | Fadia R. Homaidan Liming Zhao Iman Chakroun Carla A. Martin Robert Burakoff The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial Cells Mediators of Inflammation Interleukin-1 receptor Cytokines Prostaglandin E2 production Epithelial cells Inflammatory bowel disease. |
| title | The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial Cells |
| title_full | The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial Cells |
| title_fullStr | The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial Cells |
| title_full_unstemmed | The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial Cells |
| title_short | The Mechanisms of Action of Interleukin-1 on Rabbit Intestinal Epithelial Cells |
| title_sort | mechanisms of action of interleukin 1 on rabbit intestinal epithelial cells |
| topic | Interleukin-1 receptor Cytokines Prostaglandin E2 production Epithelial cells Inflammatory bowel disease. |
| url | http://dx.doi.org/10.1080/09629359990342 |
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