PDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer
Abstract Background Breast cancer is a heterogeneous malignancy with diverse tumor subpopulations and complex tumor-immune interactions. This study explores the prognostic and functional roles of PDE3B and HBB in breast cancer, focusing on their contributions to proliferation and immune microenviron...
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| Format: | Article |
| Language: | English |
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BMC
2025-06-01
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| Series: | Hereditas |
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| Online Access: | https://doi.org/10.1186/s41065-025-00470-z |
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| author | Bolong Yin Xiangrong Luo Xuebo Yan Hui Shen Jianping Jiang |
| author_facet | Bolong Yin Xiangrong Luo Xuebo Yan Hui Shen Jianping Jiang |
| author_sort | Bolong Yin |
| collection | DOAJ |
| description | Abstract Background Breast cancer is a heterogeneous malignancy with diverse tumor subpopulations and complex tumor-immune interactions. This study explores the prognostic and functional roles of PDE3B and HBB in breast cancer, focusing on their contributions to proliferation and immune microenvironment modulation. Methods Single-cell RNA sequencing (scRNA-seq) and TCGA data were analyzed to identify malignant subpopulations and prognostic genes. Differential gene expression, KEGG enrichment, LASSO regression, and Kaplan-Meier survival analyses were performed. Immune infiltration was assessed using EPIC deconvolution. Functional validation included qRT-PCR, IHC, Western blot, and proliferation assays in MDA-MB-231 cells. Results Malignant cell type 3 exhibited the highest proliferative potential. PDE3B and HBB were identified as prognostic markers, strongly associated with poor survival and immune cell infiltration. Overexpression of these genes enhanced proliferation, while their knockout suppressed it. Conclusion PDE3B and HBB drive breast cancer proliferation and immune modulation, making them promising biomarkers and therapeutic targets. Further research should assess their potential in targeted therapies. |
| format | Article |
| id | doaj-art-800da86f78d040668ebe8ba1b5935542 |
| institution | OA Journals |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj-art-800da86f78d040668ebe8ba1b59355422025-08-20T02:05:13ZengBMCHereditas1601-52232025-06-01162111610.1186/s41065-025-00470-zPDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancerBolong Yin0Xiangrong Luo1Xuebo Yan2Hui Shen3Jianping Jiang4The Central Hospital of ShaoyangThe Central Hospital of ShaoyangThe Central Hospital of ShaoyangThe Central Hospital of ShaoyangThe Central Hospital of ShaoyangAbstract Background Breast cancer is a heterogeneous malignancy with diverse tumor subpopulations and complex tumor-immune interactions. This study explores the prognostic and functional roles of PDE3B and HBB in breast cancer, focusing on their contributions to proliferation and immune microenvironment modulation. Methods Single-cell RNA sequencing (scRNA-seq) and TCGA data were analyzed to identify malignant subpopulations and prognostic genes. Differential gene expression, KEGG enrichment, LASSO regression, and Kaplan-Meier survival analyses were performed. Immune infiltration was assessed using EPIC deconvolution. Functional validation included qRT-PCR, IHC, Western blot, and proliferation assays in MDA-MB-231 cells. Results Malignant cell type 3 exhibited the highest proliferative potential. PDE3B and HBB were identified as prognostic markers, strongly associated with poor survival and immune cell infiltration. Overexpression of these genes enhanced proliferation, while their knockout suppressed it. Conclusion PDE3B and HBB drive breast cancer proliferation and immune modulation, making them promising biomarkers and therapeutic targets. Further research should assess their potential in targeted therapies.https://doi.org/10.1186/s41065-025-00470-zBreast cancerSingle-cell RNA-seqTCGAPrognostic biomarkersPDE3BHBB |
| spellingShingle | Bolong Yin Xiangrong Luo Xuebo Yan Hui Shen Jianping Jiang PDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer Hereditas Breast cancer Single-cell RNA-seq TCGA Prognostic biomarkers PDE3B HBB |
| title | PDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer |
| title_full | PDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer |
| title_fullStr | PDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer |
| title_full_unstemmed | PDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer |
| title_short | PDE3B and HBB are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer |
| title_sort | pde3b and hbb are key prognostic biomarkers driving cell proliferation and regulating immune microenvironment in breast cancer |
| topic | Breast cancer Single-cell RNA-seq TCGA Prognostic biomarkers PDE3B HBB |
| url | https://doi.org/10.1186/s41065-025-00470-z |
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