Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells
Olive leaves as a main byproduct of olive oil and fruit industry are a valuable source of phytochemicals such as polyphenols, with multiple biomedical effects. Apart from leaves, olive branches and stems make up a significant amount of olive waste. It is well known that the drying process and long-t...
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Sciendo
2023-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2023-0046 |
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| author | Benčić Đani Barbarić Monika Mornar Ana Klarić Daniela Amidžić Brozovic Anamaria Dabelić Sanja Fadljević Mihaela Marković Ana Karković |
| author_facet | Benčić Đani Barbarić Monika Mornar Ana Klarić Daniela Amidžić Brozovic Anamaria Dabelić Sanja Fadljević Mihaela Marković Ana Karković |
| author_sort | Benčić Đani |
| collection | DOAJ |
| description | Olive leaves as a main byproduct of olive oil and fruit industry are a valuable source of phytochemicals such as polyphenols, with multiple biomedical effects. Apart from leaves, olive branches and stems make up a significant amount of olive waste. It is well known that the drying process and long-term storage affect the stability and concentration of polyphenols present in raw materials. For that matter, two different means of storing olive waste, at room temperature and +4 °C, were compared by determining the content of the polyphenol oleuropein (OLE) in olive leaf, branch, and stem extracts (LE, BE, and SE) by HPLC-DAD method. Total phenols (TPC), o-diphenols (o-DPC), and total flavonoids (TFC) content in extracts were assessed by UV-Vis measurements. LE prepared from leaves stored at +4 °C had the highest OLE content, 30.7 mg g−1 of dry extract (DE). SE from stems stored at +4 °C was the richest in TPC and TFC (193 mg GAE/g DE and 82.9 mg CE/g DE, respectively), due to the higher purity of the extract. The biological activity of extracts was determined on cervical cancer (HeLa), melanoma (A375), metastatic melanoma (A375M) tumor cell lines, and on spontaneously immortalized cell line of keratinocytes (HaCaT), using the MTT assay. The data show that all extracts had a similar dose-dependent effect on cell viability in HeLa cells, while the effect of LE on melanoma A375 and A375M, and HaCaT cells was cell-line dependent. |
| format | Article |
| id | doaj-art-8001ed725e0d4439956b9ef63a123646 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-8001ed725e0d4439956b9ef63a1236462025-02-03T07:24:17ZengSciendoActa Pharmaceutica1846-95582023-12-0173460161610.2478/acph-2023-0046Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cellsBenčić Đani0Barbarić Monika1Mornar Ana2Klarić Daniela Amidžić3Brozovic Anamaria4Dabelić Sanja5Fadljević Mihaela6Marković Ana Karković71University of ZagrebFaculty of Agriculture, 10000Zagreb, Croatia2University of Zagreb, Faculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia2University of Zagreb, Faculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia2University of Zagreb, Faculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia3Ruđer Bošković Institute, 10000Zagreb, Croatia2University of Zagreb, Faculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia2University of Zagreb, Faculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia2University of Zagreb, Faculty of Pharmacy and Biochemistry, 10000Zagreb, CroatiaOlive leaves as a main byproduct of olive oil and fruit industry are a valuable source of phytochemicals such as polyphenols, with multiple biomedical effects. Apart from leaves, olive branches and stems make up a significant amount of olive waste. It is well known that the drying process and long-term storage affect the stability and concentration of polyphenols present in raw materials. For that matter, two different means of storing olive waste, at room temperature and +4 °C, were compared by determining the content of the polyphenol oleuropein (OLE) in olive leaf, branch, and stem extracts (LE, BE, and SE) by HPLC-DAD method. Total phenols (TPC), o-diphenols (o-DPC), and total flavonoids (TFC) content in extracts were assessed by UV-Vis measurements. LE prepared from leaves stored at +4 °C had the highest OLE content, 30.7 mg g−1 of dry extract (DE). SE from stems stored at +4 °C was the richest in TPC and TFC (193 mg GAE/g DE and 82.9 mg CE/g DE, respectively), due to the higher purity of the extract. The biological activity of extracts was determined on cervical cancer (HeLa), melanoma (A375), metastatic melanoma (A375M) tumor cell lines, and on spontaneously immortalized cell line of keratinocytes (HaCaT), using the MTT assay. The data show that all extracts had a similar dose-dependent effect on cell viability in HeLa cells, while the effect of LE on melanoma A375 and A375M, and HaCaT cells was cell-line dependent.https://doi.org/10.2478/acph-2023-0046oleuropeinolive leafolive brancholive stemlong-term storagebiological activity |
| spellingShingle | Benčić Đani Barbarić Monika Mornar Ana Klarić Daniela Amidžić Brozovic Anamaria Dabelić Sanja Fadljević Mihaela Marković Ana Karković Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells Acta Pharmaceutica oleuropein olive leaf olive branch olive stem long-term storage biological activity |
| title | Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells |
| title_full | Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells |
| title_fullStr | Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells |
| title_full_unstemmed | Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells |
| title_short | Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells |
| title_sort | oleuropein in olive leaf branch and stem extracts stability and biological activity in human cervical carcinoma and melanoma cells |
| topic | oleuropein olive leaf olive branch olive stem long-term storage biological activity |
| url | https://doi.org/10.2478/acph-2023-0046 |
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