Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies
Abstract The aim of this study is to evaluate the cell responses, potential skin reactions during the treatment process and burn wound healing efficacy of electrospun polycaprolactone/polyethylene oxide (PCL/PEO) nanofibers (NFs) containing Centella asiatica mediated synthesized silver nanoparticles...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-12-01
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| Series: | Macromolecular Materials and Engineering |
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| Online Access: | https://doi.org/10.1002/mame.202400186 |
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| author | Ogün Bozkaya Esra Bozkaya Hüsamettin Ekici Mehmet Eray Alçığır Yaşar Şahin Nebahat Aytuna Çerçi Siyami Karahan Mustafa Yiğitoğlu İbrahim Vargel |
| author_facet | Ogün Bozkaya Esra Bozkaya Hüsamettin Ekici Mehmet Eray Alçığır Yaşar Şahin Nebahat Aytuna Çerçi Siyami Karahan Mustafa Yiğitoğlu İbrahim Vargel |
| author_sort | Ogün Bozkaya |
| collection | DOAJ |
| description | Abstract The aim of this study is to evaluate the cell responses, potential skin reactions during the treatment process and burn wound healing efficacy of electrospun polycaprolactone/polyethylene oxide (PCL/PEO) nanofibers (NFs) containing Centella asiatica mediated synthesized silver nanoparticles (CA‐AgNPs) by in vitro and in vivo studies. Apoptosis‐necrosis, genotoxicity, hemolysis, and cell attachment studies are carried out within the scope of in vitro tests, and irritation, sensitivity, and burn wound studies are carried out within the scope of in vivo tests. The apoptotic index value of CA‐AgNPs‐[PCL/PEO] NFs material on L929 fibroblast cells is determined as 5.0 ± 1.0% at the highest concentration and the necrotic index value is 5.0 ± 0.3%. Micronucleus rates (%) of NFs treated with CHO (Chinese Hamster Ovary) cells are not at genotoxic level. The hemolytic index value of NFs dressing is determined as 0.23 ± 0.03%, The primary irritation index (PII) value of NFs wound dressing is calculated as 0.36 by irritation tests. In addition, the potential sensitization reaction of NFs extract on guinea pigs is evaluated and the sensitization score is determined as 0.9. The healing efficacy of NFs material on second‐degree burn wounds compared to a commercial product is supported by pathomorphological findings. |
| format | Article |
| id | doaj-art-800144bfcb484a32b80d3781d244e56f |
| institution | OA Journals |
| issn | 1438-7492 1439-2054 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Macromolecular Materials and Engineering |
| spelling | doaj-art-800144bfcb484a32b80d3781d244e56f2025-08-20T01:58:20ZengWiley-VCHMacromolecular Materials and Engineering1438-74921439-20542024-12-0130912n/an/a10.1002/mame.202400186Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo StudiesOgün Bozkaya0Esra Bozkaya1Hüsamettin Ekici2Mehmet Eray Alçığır3Yaşar Şahin4Nebahat Aytuna Çerçi5Siyami Karahan6Mustafa Yiğitoğlu7İbrahim Vargel8Institute of Science Department of Bioengineering Hacettepe University Ankara 06800 TurkeyScientific and Technological Research Application and Research Center Health Sciences and Biotechnology Laboratories Kırıkkale University Kırıkkale 71450 TurkeyFaculty of Veterinary Medicine Department of Pharmacology and Toxicology Kırıkkale University Kırıkkale 71450 TurkeyFaculty of Veterinary Medicine Department of Veterinary Pathology Kırıkkale University Kırıkkale 71450 TurkeyFaculty of Veterinary Medicine Department of Pharmacology and Toxicology Kırıkkale University Kırıkkale 71450 TurkeyScientific and Technological Research Application and Research Center Health Sciences and Biotechnology Laboratories Kırıkkale University Kırıkkale 71450 TurkeyFaculty of Veterinary Medicine Department of Histology and Embryology Kırıkkale University Kırıkkale 71450 TurkeyFaculty of Engineering and Natural Sciences Department of Bioengineering Kırıkkale University Kırıkkale 71450 TurkeyFaculty of Medicine Department of Plastic and Reconstructive Surgery Hacettepe University Ankara 06800 TurkeyAbstract The aim of this study is to evaluate the cell responses, potential skin reactions during the treatment process and burn wound healing efficacy of electrospun polycaprolactone/polyethylene oxide (PCL/PEO) nanofibers (NFs) containing Centella asiatica mediated synthesized silver nanoparticles (CA‐AgNPs) by in vitro and in vivo studies. Apoptosis‐necrosis, genotoxicity, hemolysis, and cell attachment studies are carried out within the scope of in vitro tests, and irritation, sensitivity, and burn wound studies are carried out within the scope of in vivo tests. The apoptotic index value of CA‐AgNPs‐[PCL/PEO] NFs material on L929 fibroblast cells is determined as 5.0 ± 1.0% at the highest concentration and the necrotic index value is 5.0 ± 0.3%. Micronucleus rates (%) of NFs treated with CHO (Chinese Hamster Ovary) cells are not at genotoxic level. The hemolytic index value of NFs dressing is determined as 0.23 ± 0.03%, The primary irritation index (PII) value of NFs wound dressing is calculated as 0.36 by irritation tests. In addition, the potential sensitization reaction of NFs extract on guinea pigs is evaluated and the sensitization score is determined as 0.9. The healing efficacy of NFs material on second‐degree burn wounds compared to a commercial product is supported by pathomorphological findings.https://doi.org/10.1002/mame.202400186biocompatibilityburn woundCentella asiaticawound dressingwound healing |
| spellingShingle | Ogün Bozkaya Esra Bozkaya Hüsamettin Ekici Mehmet Eray Alçığır Yaşar Şahin Nebahat Aytuna Çerçi Siyami Karahan Mustafa Yiğitoğlu İbrahim Vargel Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies Macromolecular Materials and Engineering biocompatibility burn wound Centella asiatica wound dressing wound healing |
| title | Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies |
| title_full | Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies |
| title_fullStr | Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies |
| title_full_unstemmed | Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies |
| title_short | Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies |
| title_sort | evaluation of burn wound healing efficacy and biocompatibility of centella asiatica mediated synthesised agnps loaded hybrid nanofiber scaffold in vitro and in vivo studies |
| topic | biocompatibility burn wound Centella asiatica wound dressing wound healing |
| url | https://doi.org/10.1002/mame.202400186 |
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