Effect of pH on Physicochemical Properties and Encapsulation Efficiency of PEGylated Linolenic Acid Vesicles
The preparation of vesicle from a mixture of linolenic acid and 1,2-dipalmitoyl-sn-glycerol-3-phosphoethanolamine-N-[methoxy-(polyethylene glycol) -2000] (DPPE-PEG2000) has been successfully carried out by using dry lipid hydration method. The effect of pH on particle size, zeta potential, encapsula...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/421286 |
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| author | Yin Yin Teo Misni Misran Kah Hin Low |
| author_facet | Yin Yin Teo Misni Misran Kah Hin Low |
| author_sort | Yin Yin Teo |
| collection | DOAJ |
| description | The preparation of vesicle from a mixture of linolenic acid and 1,2-dipalmitoyl-sn-glycerol-3-phosphoethanolamine-N-[methoxy-(polyethylene glycol) -2000] (DPPE-PEG2000) has been successfully carried out by using dry lipid hydration method. The effect of pH on particle size, zeta potential, encapsulation efficiency and critical vesiculation concentration (CVC) of the prepared vesicle suspensions in aqueous were studied. Macroscopic stability of the vesicles was also evaluated through their particle size and zeta potential for a period of 30 days. We found that CVC vary according to the pH, with higher pH of the bulk solution, CVC is higher. Vesicles formed at pH 8.5 were the most stable suspension throughout a period of 30 days compared to those at pH 7.5 and pH 9.0. Addition of DPPE-PEG2000 into the preparation of vesicle at pH 8.5 caused a reduction of the vesicle size to the scale of nanometer which is an advantage to their application. On the other hand, encapsulation of calcein and vitamin E were carried out. Certain amount of these compounds could be successfully loaded into the resulting liposomes under this experimental condition. |
| format | Article |
| id | doaj-art-7fff8a39202147798e468af9d11739fe |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
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| series | E-Journal of Chemistry |
| spelling | doaj-art-7fff8a39202147798e468af9d11739fe2025-02-03T05:54:29ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-019272973810.1155/2012/421286Effect of pH on Physicochemical Properties and Encapsulation Efficiency of PEGylated Linolenic Acid VesiclesYin Yin Teo0Misni Misran1Kah Hin Low2Department of Chemistry, Faculty of Science, University Malaya 50603 Kuala Lumpur, MalaysiaDepartment of Chemistry, Faculty of Science, University Malaya 50603 Kuala Lumpur, MalaysiaDepartment of Chemistry, Faculty of Science, University Malaya 50603 Kuala Lumpur, MalaysiaThe preparation of vesicle from a mixture of linolenic acid and 1,2-dipalmitoyl-sn-glycerol-3-phosphoethanolamine-N-[methoxy-(polyethylene glycol) -2000] (DPPE-PEG2000) has been successfully carried out by using dry lipid hydration method. The effect of pH on particle size, zeta potential, encapsulation efficiency and critical vesiculation concentration (CVC) of the prepared vesicle suspensions in aqueous were studied. Macroscopic stability of the vesicles was also evaluated through their particle size and zeta potential for a period of 30 days. We found that CVC vary according to the pH, with higher pH of the bulk solution, CVC is higher. Vesicles formed at pH 8.5 were the most stable suspension throughout a period of 30 days compared to those at pH 7.5 and pH 9.0. Addition of DPPE-PEG2000 into the preparation of vesicle at pH 8.5 caused a reduction of the vesicle size to the scale of nanometer which is an advantage to their application. On the other hand, encapsulation of calcein and vitamin E were carried out. Certain amount of these compounds could be successfully loaded into the resulting liposomes under this experimental condition.http://dx.doi.org/10.1155/2012/421286 |
| spellingShingle | Yin Yin Teo Misni Misran Kah Hin Low Effect of pH on Physicochemical Properties and Encapsulation Efficiency of PEGylated Linolenic Acid Vesicles E-Journal of Chemistry |
| title | Effect of pH on Physicochemical Properties and Encapsulation Efficiency of PEGylated Linolenic Acid Vesicles |
| title_full | Effect of pH on Physicochemical Properties and Encapsulation Efficiency of PEGylated Linolenic Acid Vesicles |
| title_fullStr | Effect of pH on Physicochemical Properties and Encapsulation Efficiency of PEGylated Linolenic Acid Vesicles |
| title_full_unstemmed | Effect of pH on Physicochemical Properties and Encapsulation Efficiency of PEGylated Linolenic Acid Vesicles |
| title_short | Effect of pH on Physicochemical Properties and Encapsulation Efficiency of PEGylated Linolenic Acid Vesicles |
| title_sort | effect of ph on physicochemical properties and encapsulation efficiency of pegylated linolenic acid vesicles |
| url | http://dx.doi.org/10.1155/2012/421286 |
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