Identification of mitophagy-related genes in patients with acute myocardial infarction

Abstract Mitophagy is involved in acute myocardial infarction (AMI) process. However, the role of mitophagy-related genes (MRGs) in the AMI process is not well illustrated. We identified MRGs involved in AMI by bioinformatics analysis. The external datasets were employed for the validation of the MR...

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Main Authors: Ju-Ying Li, Hong-Kui Chen, Yi-Hao Huang, Yu-Peng Zhi, Yue-E Li, Kai-Yang Lin, Chun Chen, Yan-Song Guo
Format: Article
Language:English
Published: BMC 2025-04-01
Series:Hereditas
Online Access:https://doi.org/10.1186/s41065-025-00424-5
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author Ju-Ying Li
Hong-Kui Chen
Yi-Hao Huang
Yu-Peng Zhi
Yue-E Li
Kai-Yang Lin
Chun Chen
Yan-Song Guo
author_facet Ju-Ying Li
Hong-Kui Chen
Yi-Hao Huang
Yu-Peng Zhi
Yue-E Li
Kai-Yang Lin
Chun Chen
Yan-Song Guo
author_sort Ju-Ying Li
collection DOAJ
description Abstract Mitophagy is involved in acute myocardial infarction (AMI) process. However, the role of mitophagy-related genes (MRGs) in the AMI process is not well illustrated. We identified MRGs involved in AMI by bioinformatics analysis. The external datasets were employed for the validation of the MRGs, alongside the execution of cellular and animal experiments. Forty-five MRGs were detected, and machine learning identified the top four hub genes, namely ALDH2, ACSL1, IL1B, and GABARAPL1. Additionally, an external validation set was used to screen for three diagnostic markers (ACSL1, IL1B, and GABARAPL1) among these hub genes. Immune infiltration analysis revealed changes in the immune microenvironment among patients with AMI. Finally, the significant upregulation of ACSL1, IL1B, and GABARAPL1 in both cellular and animal models was confirmed. The occurrence of mitophagy was observed in the cell model through transmission electron microscopy (TEM). Our study demonstrated that ACSL1, IL1B, and GABARAPL1 possess potential biomarkers for AMI.
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issn 1601-5223
language English
publishDate 2025-04-01
publisher BMC
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series Hereditas
spelling doaj-art-7ff28bd6745a49e98698a99b6a1537c32025-08-20T03:15:12ZengBMCHereditas1601-52232025-04-01162111410.1186/s41065-025-00424-5Identification of mitophagy-related genes in patients with acute myocardial infarctionJu-Ying Li0Hong-Kui Chen1Yi-Hao Huang2Yu-Peng Zhi3Yue-E Li4Kai-Yang Lin5Chun Chen6Yan-Song Guo7The First People’s Hospital of YibinShenli Clincal Medical College of Fujian Medical UniversitySchool of Pharmacy, Fujian Medical UniversitySchool of Pharmacy, Fujian Medical UniversitySchool of Pharmacy, Fujian Medical UniversityShenli Clincal Medical College of Fujian Medical UniversitySchool of Pharmacy, Fujian Medical UniversityShenli Clincal Medical College of Fujian Medical UniversityAbstract Mitophagy is involved in acute myocardial infarction (AMI) process. However, the role of mitophagy-related genes (MRGs) in the AMI process is not well illustrated. We identified MRGs involved in AMI by bioinformatics analysis. The external datasets were employed for the validation of the MRGs, alongside the execution of cellular and animal experiments. Forty-five MRGs were detected, and machine learning identified the top four hub genes, namely ALDH2, ACSL1, IL1B, and GABARAPL1. Additionally, an external validation set was used to screen for three diagnostic markers (ACSL1, IL1B, and GABARAPL1) among these hub genes. Immune infiltration analysis revealed changes in the immune microenvironment among patients with AMI. Finally, the significant upregulation of ACSL1, IL1B, and GABARAPL1 in both cellular and animal models was confirmed. The occurrence of mitophagy was observed in the cell model through transmission electron microscopy (TEM). Our study demonstrated that ACSL1, IL1B, and GABARAPL1 possess potential biomarkers for AMI.https://doi.org/10.1186/s41065-025-00424-5
spellingShingle Ju-Ying Li
Hong-Kui Chen
Yi-Hao Huang
Yu-Peng Zhi
Yue-E Li
Kai-Yang Lin
Chun Chen
Yan-Song Guo
Identification of mitophagy-related genes in patients with acute myocardial infarction
Hereditas
title Identification of mitophagy-related genes in patients with acute myocardial infarction
title_full Identification of mitophagy-related genes in patients with acute myocardial infarction
title_fullStr Identification of mitophagy-related genes in patients with acute myocardial infarction
title_full_unstemmed Identification of mitophagy-related genes in patients with acute myocardial infarction
title_short Identification of mitophagy-related genes in patients with acute myocardial infarction
title_sort identification of mitophagy related genes in patients with acute myocardial infarction
url https://doi.org/10.1186/s41065-025-00424-5
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