Vitamin A-loaded decellularized kidney capsule promoted wound healing in rat
Tissue regeneration in many skin defects is progressing with new treatments in recent years. Tissue engineering with the use of scaffolds offers more versatile and faster solutions in treatment. Extracellular matrix (ECM) and its three-dimensional (3D) network structure as a biological bond by imita...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424001780 |
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| author | Mozafar Khazaei Mohammad Rasool Khazaei Sepideh Kadivarian Shahid Muhsin Hama Hevar Hassan Hussein Hojjat Haghighimanesh Leila Rezakhani |
| author_facet | Mozafar Khazaei Mohammad Rasool Khazaei Sepideh Kadivarian Shahid Muhsin Hama Hevar Hassan Hussein Hojjat Haghighimanesh Leila Rezakhani |
| author_sort | Mozafar Khazaei |
| collection | DOAJ |
| description | Tissue regeneration in many skin defects is progressing with new treatments in recent years. Tissue engineering with the use of scaffolds offers more versatile and faster solutions in treatment. Extracellular matrix (ECM) and its three-dimensional (3D) network structure as a biological bond by imitating the tissue microstructure has been used for tissue repair, which can answer many existing challenges. Vitamin A, which comes in several forms such as retinols, retinals, and retinoic acids, is a necessary vitamin that is crucial for wound healing. In this research, sheep kidney capsule tissue decellularized with sodium dodecyl sulfate (SDS) containing different doses of vitamin A has been used as an ECM in skin tissue engineering. The above scaffold was evaluated in terms of properties such as biocompatibility, analysis of mechanical properties, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), hydrophilicity, antibacterial, and cell adhesion. The findings reported suitable properties for wound dressing, especially at a dose of 15,000 U/ml vitamin A for this scaffold. Then, the above scaffold was evaluated on the full-thickness wound model in rat, which showed good wound contraction, and increased VEGF factor. It showed a decrease in IL-1β level. Therefore, the use of the above-mentioned decellularized scaffold in combination with medicinal agents effective in wound healing can be introduced for further pre-clinical studies. |
| format | Article |
| id | doaj-art-7fec30a793cb4e89b6e90b16de626f49 |
| institution | OA Journals |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-7fec30a793cb4e89b6e90b16de626f492025-08-20T02:07:31ZengElsevierRegenerative Therapy2352-32042024-06-012686787810.1016/j.reth.2024.09.017Vitamin A-loaded decellularized kidney capsule promoted wound healing in ratMozafar Khazaei0Mohammad Rasool Khazaei1Sepideh Kadivarian2Shahid Muhsin Hama3Hevar Hassan Hussein4Hojjat Haghighimanesh5Leila Rezakhani6Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Tissue Engineering, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranFertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Tissue Engineering, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranStudent Research Committee, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IranStudent Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IranStudent Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Plastic & Reconstructive Surgery, School of Medicine, Guilan University of Medical Sciences, Rasht, IranFertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Tissue Engineering, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Corresponding author. Department of tissue engineering, school of medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.Tissue regeneration in many skin defects is progressing with new treatments in recent years. Tissue engineering with the use of scaffolds offers more versatile and faster solutions in treatment. Extracellular matrix (ECM) and its three-dimensional (3D) network structure as a biological bond by imitating the tissue microstructure has been used for tissue repair, which can answer many existing challenges. Vitamin A, which comes in several forms such as retinols, retinals, and retinoic acids, is a necessary vitamin that is crucial for wound healing. In this research, sheep kidney capsule tissue decellularized with sodium dodecyl sulfate (SDS) containing different doses of vitamin A has been used as an ECM in skin tissue engineering. The above scaffold was evaluated in terms of properties such as biocompatibility, analysis of mechanical properties, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), hydrophilicity, antibacterial, and cell adhesion. The findings reported suitable properties for wound dressing, especially at a dose of 15,000 U/ml vitamin A for this scaffold. Then, the above scaffold was evaluated on the full-thickness wound model in rat, which showed good wound contraction, and increased VEGF factor. It showed a decrease in IL-1β level. Therefore, the use of the above-mentioned decellularized scaffold in combination with medicinal agents effective in wound healing can be introduced for further pre-clinical studies.http://www.sciencedirect.com/science/article/pii/S2352320424001780Kidney capsuleVitamin ADecellularizationWound healing |
| spellingShingle | Mozafar Khazaei Mohammad Rasool Khazaei Sepideh Kadivarian Shahid Muhsin Hama Hevar Hassan Hussein Hojjat Haghighimanesh Leila Rezakhani Vitamin A-loaded decellularized kidney capsule promoted wound healing in rat Regenerative Therapy Kidney capsule Vitamin A Decellularization Wound healing |
| title | Vitamin A-loaded decellularized kidney capsule promoted wound healing in rat |
| title_full | Vitamin A-loaded decellularized kidney capsule promoted wound healing in rat |
| title_fullStr | Vitamin A-loaded decellularized kidney capsule promoted wound healing in rat |
| title_full_unstemmed | Vitamin A-loaded decellularized kidney capsule promoted wound healing in rat |
| title_short | Vitamin A-loaded decellularized kidney capsule promoted wound healing in rat |
| title_sort | vitamin a loaded decellularized kidney capsule promoted wound healing in rat |
| topic | Kidney capsule Vitamin A Decellularization Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424001780 |
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