The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP
AimThis study aimed to investigate the regenerative effects of PRP on an experimental rat model of disc degeneration using histological and biochemical parameters. Additionally, we evaluated whether ropivacaine, a local anesthetic commonly used in clinical practice, affects the efficacy of PRP.Metho...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1613148/full |
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| author | Ahmet Mert Ayse Ikinci Keles Murat Aydin Huseyin Serkan Erol Osman Fikret Sonmez |
| author_facet | Ahmet Mert Ayse Ikinci Keles Murat Aydin Huseyin Serkan Erol Osman Fikret Sonmez |
| author_sort | Ahmet Mert |
| collection | DOAJ |
| description | AimThis study aimed to investigate the regenerative effects of PRP on an experimental rat model of disc degeneration using histological and biochemical parameters. Additionally, we evaluated whether ropivacaine, a local anesthetic commonly used in clinical practice, affects the efficacy of PRP.MethodsRats were randomly divided into five groups as control and treatment groups. Disc degeneration models were established using appropriate procedures. On the intervention day, PRP was prepared from whole blood collected from the rats. PRP, PRP + ropivacaine, or ropivacaine alone was administered at the appropriate doses and according to standardized protocols.ResultsIn the untreated disc degeneration groups, annulus fibrosus (AF) and nucleus pulposus (NP) boundaries were indistinct, disc morphology was disrupted, collagen structures in the AF were degenerated or irregular, and vacuolization, interstitial edema, and necrotic tissue remnants were observed in the NP region. In contrast, in groups treated with PRP and PRP + ropivacaine, a reduction in edema and vacuolization, disappearance of necrotic tissue, restoration of distinct NP and AF boundaries, and decreased atrophy and cellular clustering in NP cells were observed. Biochemical analysis showed that IL-6 and TNF-α levels were within normal ranges in the groups treated with PRP and PRP + ropivacaine, whereas these levels remained elevated in the untreated disc degeneration groups, indicating ongoing effects of degeneration.ConclusionThis study demonstrates the regenerative effects of PRP in disc degeneration through histological and biochemical parameters. Furthermore, the addition of ropivacaine to PRP did not exert any negative effects on PRP’s regenerative properties. |
| format | Article |
| id | doaj-art-7fe232a0b15e43dfb809b0284ad34b38 |
| institution | OA Journals |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-7fe232a0b15e43dfb809b0284ad34b382025-08-20T02:07:27ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-06-011310.3389/fbioe.2025.16131481613148The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRPAhmet Mert0Ayse Ikinci Keles1Murat Aydin2Huseyin Serkan Erol3Osman Fikret Sonmez4Department of Orthopedics and Traumatology, Private Pendik Yüzyıl Hospital, Istanbul, TürkiyeDepartment of Histology and Embryology, Aksaray University, Faculty of Medicine, Aksaray, TürkiyeDepartment of Orthopedics and Traumatology, Private Buyuk Anadolu Hospital, Istanbul, TürkiyeDepartment of Biochemistry, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, TürkiyeDepartment of Neurosurgery, Tepecik Training and Research Hospital, Izmir, TürkiyeAimThis study aimed to investigate the regenerative effects of PRP on an experimental rat model of disc degeneration using histological and biochemical parameters. Additionally, we evaluated whether ropivacaine, a local anesthetic commonly used in clinical practice, affects the efficacy of PRP.MethodsRats were randomly divided into five groups as control and treatment groups. Disc degeneration models were established using appropriate procedures. On the intervention day, PRP was prepared from whole blood collected from the rats. PRP, PRP + ropivacaine, or ropivacaine alone was administered at the appropriate doses and according to standardized protocols.ResultsIn the untreated disc degeneration groups, annulus fibrosus (AF) and nucleus pulposus (NP) boundaries were indistinct, disc morphology was disrupted, collagen structures in the AF were degenerated or irregular, and vacuolization, interstitial edema, and necrotic tissue remnants were observed in the NP region. In contrast, in groups treated with PRP and PRP + ropivacaine, a reduction in edema and vacuolization, disappearance of necrotic tissue, restoration of distinct NP and AF boundaries, and decreased atrophy and cellular clustering in NP cells were observed. Biochemical analysis showed that IL-6 and TNF-α levels were within normal ranges in the groups treated with PRP and PRP + ropivacaine, whereas these levels remained elevated in the untreated disc degeneration groups, indicating ongoing effects of degeneration.ConclusionThis study demonstrates the regenerative effects of PRP in disc degeneration through histological and biochemical parameters. Furthermore, the addition of ropivacaine to PRP did not exert any negative effects on PRP’s regenerative properties.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1613148/fullinflammationintervertebral disc degenerationlocal anesthesiaplatelet-rich plasmarat |
| spellingShingle | Ahmet Mert Ayse Ikinci Keles Murat Aydin Huseyin Serkan Erol Osman Fikret Sonmez The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP Frontiers in Bioengineering and Biotechnology inflammation intervertebral disc degeneration local anesthesia platelet-rich plasma rat |
| title | The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP |
| title_full | The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP |
| title_fullStr | The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP |
| title_full_unstemmed | The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP |
| title_short | The role of PRP in the healing of disc degeneration and the effect of local anesthetics on PRP |
| title_sort | role of prp in the healing of disc degeneration and the effect of local anesthetics on prp |
| topic | inflammation intervertebral disc degeneration local anesthesia platelet-rich plasma rat |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1613148/full |
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