Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerol
ABSTRACT During microbial industrial production, microorganisms often face diverse stressors, including organic solvents, high salinity, and high sugar levels. Enhancing microorganism tolerance to such stresses is crucial for producing high-value-added products. Previous studies on the mechanisms of...
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00089-24 |
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| author | Zixiong Liu Lingling Shangguan Linglong Xu Huiyan Zhang Wenxin Wang Qiao Yang Xiaoling Zhang Lan Yao Shihui Yang Xiong Chen Jun Dai |
| author_facet | Zixiong Liu Lingling Shangguan Linglong Xu Huiyan Zhang Wenxin Wang Qiao Yang Xiaoling Zhang Lan Yao Shihui Yang Xiong Chen Jun Dai |
| author_sort | Zixiong Liu |
| collection | DOAJ |
| description | ABSTRACT During microbial industrial production, microorganisms often face diverse stressors, including organic solvents, high salinity, and high sugar levels. Enhancing microorganism tolerance to such stresses is crucial for producing high-value-added products. Previous studies on the mechanisms of 2-phenylethanol (2-PE) tolerance in Saccharomyces cerevisiae revealed a potential connection between the sugar transporter-like protein (Stl1) mutation (F427L) and increased tolerance to high sugar and salt stress, suggesting a broader role in multistress tolerance. Herein, we showed that the Stl1F427L mutant strain (STL) exhibits significantly improved multistress tolerance in the presence of glycerol. Molecular dynamics simulations indicated that Stl1F427L may enhance glycerol molecular binding, resulting in a significant increase in the intracellular glycerol content of the mutant strain STL. Additionally, under multistress conditions, pyruvate and ergosterol levels and catalase (CAT) and superoxide dismutase (SOD) activities were significantly increased in the mutant strain STL compared with the control strain 5D. This resulted in a notable increase in cell membrane toughness and a decrease in intracellular reactive oxygen species levels. These findings highlight the mechanism by which Stl1F427L enhances S. cerevisiae tolerance to multistress. Importantly, they provide novel insights into and methodologies for improving the resilience of industrial microorganisms.IMPORTANCEStl1F427L exhibits improved strain tolerance to multistress when adding glycerol, may enhance glycerol molecular binding, and can make a significant increase in intracellular glycerol content. It can reduce reactive oxygen species levels and increase ergosterol content. This paper provides novel insights and methods to get robust industrial microorganisms. |
| format | Article |
| id | doaj-art-7fd2a568039546aa80a0d4cf52f0cf87 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-7fd2a568039546aa80a0d4cf52f0cf872025-02-04T14:03:41ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.00089-24Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerolZixiong Liu0Lingling Shangguan1Linglong Xu2Huiyan Zhang3Wenxin Wang4Qiao Yang5Xiaoling Zhang6Lan Yao7Shihui Yang8Xiong Chen9Jun Dai10Key Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Bioengineering and Food Science, Hubei University of Technology, Wuhan, Hubei, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Bioengineering and Food Science, Hubei University of Technology, Wuhan, Hubei, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Bioengineering and Food Science, Hubei University of Technology, Wuhan, Hubei, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Bioengineering and Food Science, Hubei University of Technology, Wuhan, Hubei, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Bioengineering and Food Science, Hubei University of Technology, Wuhan, Hubei, ChinaABI Group, Donghai Laboratory, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, Zhejiang, ChinaABI Group, Donghai Laboratory, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, Zhejiang, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Bioengineering and Food Science, Hubei University of Technology, Wuhan, Hubei, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Bioengineering and Food Science, Hubei University of Technology, Wuhan, Hubei, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Bioengineering and Food Science, Hubei University of Technology, Wuhan, Hubei, ChinaABSTRACT During microbial industrial production, microorganisms often face diverse stressors, including organic solvents, high salinity, and high sugar levels. Enhancing microorganism tolerance to such stresses is crucial for producing high-value-added products. Previous studies on the mechanisms of 2-phenylethanol (2-PE) tolerance in Saccharomyces cerevisiae revealed a potential connection between the sugar transporter-like protein (Stl1) mutation (F427L) and increased tolerance to high sugar and salt stress, suggesting a broader role in multistress tolerance. Herein, we showed that the Stl1F427L mutant strain (STL) exhibits significantly improved multistress tolerance in the presence of glycerol. Molecular dynamics simulations indicated that Stl1F427L may enhance glycerol molecular binding, resulting in a significant increase in the intracellular glycerol content of the mutant strain STL. Additionally, under multistress conditions, pyruvate and ergosterol levels and catalase (CAT) and superoxide dismutase (SOD) activities were significantly increased in the mutant strain STL compared with the control strain 5D. This resulted in a notable increase in cell membrane toughness and a decrease in intracellular reactive oxygen species levels. These findings highlight the mechanism by which Stl1F427L enhances S. cerevisiae tolerance to multistress. Importantly, they provide novel insights into and methodologies for improving the resilience of industrial microorganisms.IMPORTANCEStl1F427L exhibits improved strain tolerance to multistress when adding glycerol, may enhance glycerol molecular binding, and can make a significant increase in intracellular glycerol content. It can reduce reactive oxygen species levels and increase ergosterol content. This paper provides novel insights and methods to get robust industrial microorganisms.https://journals.asm.org/doi/10.1128/spectrum.00089-24multistress tolerancesugar transporter-like protein mutationSaccharomyces cerevisiaeglycerol |
| spellingShingle | Zixiong Liu Lingling Shangguan Linglong Xu Huiyan Zhang Wenxin Wang Qiao Yang Xiaoling Zhang Lan Yao Shihui Yang Xiong Chen Jun Dai Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerol Microbiology Spectrum multistress tolerance sugar transporter-like protein mutation Saccharomyces cerevisiae glycerol |
| title | Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerol |
| title_full | Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerol |
| title_fullStr | Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerol |
| title_full_unstemmed | Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerol |
| title_short | Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerol |
| title_sort | enhanced multistress tolerance of saccharomyces cerevisiae with the sugar transporter like protein stl1f427l mutation in the presence of glycerol |
| topic | multistress tolerance sugar transporter-like protein mutation Saccharomyces cerevisiae glycerol |
| url | https://journals.asm.org/doi/10.1128/spectrum.00089-24 |
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