Reduced Proliferative Potential with Conserved Stem/Stromal Phenotype of Human Umbilical Cord Mesenchymal Stem Cells in Placental Syndromes: A Prospective Cohort Study
Background: Pathophysiology of placental syndromes is still unclear, and umbilical cord-derived mesenchymal stem cells (UC-MSCs) might play a role in the development of these syndromes. In this prospective cohort study, we evaluated proliferative abilities of two types of UC-MSCs, Wharton’s Jelly MS...
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IMR Press
2023-09-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/50/9/10.31083/j.ceog5009196 |
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| author | Luigi Marino Maria Antonietta Castaldi Caterina Fulgione Salvatore Giovanni Castaldi Paola Manzo Valentina Giudice Francesca Picone Maria Rosaria Campitiello Mario Polichetti Maurizio Guida Carmine Selleri Bianca Serio |
| author_facet | Luigi Marino Maria Antonietta Castaldi Caterina Fulgione Salvatore Giovanni Castaldi Paola Manzo Valentina Giudice Francesca Picone Maria Rosaria Campitiello Mario Polichetti Maurizio Guida Carmine Selleri Bianca Serio |
| author_sort | Luigi Marino |
| collection | DOAJ |
| description | Background: Pathophysiology of placental syndromes is still unclear, and umbilical cord-derived mesenchymal stem cells (UC-MSCs) might play a role in the development of these syndromes. In this prospective cohort study, we evaluated proliferative abilities of two types of UC-MSCs, Wharton’s Jelly MSCs (WJ-MSCs) and cord blood MSCs (CB-MSCs), in placental syndromes. Methods: A total of 16 cord blood and umbilical cord samples were seeded and cultured until MSC growth potential exhaustion. Cumulative population doublings were employed for studying growth potential, and flow cytometry immunophenotyping for verification of mesenchymal markers. Results: In our prospective cohort study, on one hand CB-MSCs from pathological pregnancies showed a significant reduction of growth potential, on the other hand WJ-MSCs showed a trend toward higher growth potential. This trend is consistent with the well-known faster-growing phenotype of WJ-MSCs under low oxygen atmosphere. Moreover, it’s well understood that chronic hypoxia is a main feature of both intrauterine growth restriction (IUGR) and preeclampsia, thus, our data perfectly match with the well-known clinical characteristics. Conclusions: Growth potential of CB-MSCs obtained from placental syndromes tended to be reduced compared to that of MSCs from healthy pregnancies. Our results need to be confirmed in larger in vitro studies, as a higher number of CB- and WJ-MSC would better clarify pathophysiology of placental syndromes. |
| format | Article |
| id | doaj-art-7fcea3cfef5f4353ae3f01e4b480cba2 |
| institution | Kabale University |
| issn | 0390-6663 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj-art-7fcea3cfef5f4353ae3f01e4b480cba22025-08-20T03:35:20ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632023-09-0150919610.31083/j.ceog5009196S0390-6663(23)02150-4Reduced Proliferative Potential with Conserved Stem/Stromal Phenotype of Human Umbilical Cord Mesenchymal Stem Cells in Placental Syndromes: A Prospective Cohort StudyLuigi Marino0Maria Antonietta Castaldi1Caterina Fulgione2Salvatore Giovanni Castaldi3Paola Manzo4Valentina Giudice5Francesca Picone6Maria Rosaria Campitiello7Mario Polichetti8Maurizio Guida9Carmine Selleri10Bianca Serio11Department of Medicine, Surgery and Dentistry, University of Salerno, 84084 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry, University of Salerno, 84084 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry, University of Salerno, 84084 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry, University of Salerno, 84084 Baronissi, ItalyHematology and Transplant Center, University Hospital “San Giovanni di Dio e Ruggi d’Aragona”, 84131 Salerno, ItalyDepartment of Medicine, Surgery and Dentistry, University of Salerno, 84084 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry, University of Salerno, 84084 Baronissi, ItalyHealth Department, National Health Institute, 00161 Rome, ItalyGynecology and Obstetrics Unit, University Hospital “San Giovanni di Dio e Ruggi d’Aragona”, 84131 Salerno, ItalyGynecology Department, University of Naples “Federico II”, 80138 Naples, ItalyDepartment of Medicine, Surgery and Dentistry, University of Salerno, 84084 Baronissi, ItalyHematology and Transplant Center, University Hospital “San Giovanni di Dio e Ruggi d’Aragona”, 84131 Salerno, ItalyBackground: Pathophysiology of placental syndromes is still unclear, and umbilical cord-derived mesenchymal stem cells (UC-MSCs) might play a role in the development of these syndromes. In this prospective cohort study, we evaluated proliferative abilities of two types of UC-MSCs, Wharton’s Jelly MSCs (WJ-MSCs) and cord blood MSCs (CB-MSCs), in placental syndromes. Methods: A total of 16 cord blood and umbilical cord samples were seeded and cultured until MSC growth potential exhaustion. Cumulative population doublings were employed for studying growth potential, and flow cytometry immunophenotyping for verification of mesenchymal markers. Results: In our prospective cohort study, on one hand CB-MSCs from pathological pregnancies showed a significant reduction of growth potential, on the other hand WJ-MSCs showed a trend toward higher growth potential. This trend is consistent with the well-known faster-growing phenotype of WJ-MSCs under low oxygen atmosphere. Moreover, it’s well understood that chronic hypoxia is a main feature of both intrauterine growth restriction (IUGR) and preeclampsia, thus, our data perfectly match with the well-known clinical characteristics. Conclusions: Growth potential of CB-MSCs obtained from placental syndromes tended to be reduced compared to that of MSCs from healthy pregnancies. Our results need to be confirmed in larger in vitro studies, as a higher number of CB- and WJ-MSC would better clarify pathophysiology of placental syndromes.https://www.imrpress.com/journal/CEOG/50/9/10.31083/j.ceog5009196mesenchymal stem cellswharton's jellycord bloodplacental syndromesintrauterine growth restriction |
| spellingShingle | Luigi Marino Maria Antonietta Castaldi Caterina Fulgione Salvatore Giovanni Castaldi Paola Manzo Valentina Giudice Francesca Picone Maria Rosaria Campitiello Mario Polichetti Maurizio Guida Carmine Selleri Bianca Serio Reduced Proliferative Potential with Conserved Stem/Stromal Phenotype of Human Umbilical Cord Mesenchymal Stem Cells in Placental Syndromes: A Prospective Cohort Study Clinical and Experimental Obstetrics & Gynecology mesenchymal stem cells wharton's jelly cord blood placental syndromes intrauterine growth restriction |
| title | Reduced Proliferative Potential with Conserved Stem/Stromal Phenotype of Human Umbilical Cord Mesenchymal Stem Cells in Placental Syndromes: A Prospective Cohort Study |
| title_full | Reduced Proliferative Potential with Conserved Stem/Stromal Phenotype of Human Umbilical Cord Mesenchymal Stem Cells in Placental Syndromes: A Prospective Cohort Study |
| title_fullStr | Reduced Proliferative Potential with Conserved Stem/Stromal Phenotype of Human Umbilical Cord Mesenchymal Stem Cells in Placental Syndromes: A Prospective Cohort Study |
| title_full_unstemmed | Reduced Proliferative Potential with Conserved Stem/Stromal Phenotype of Human Umbilical Cord Mesenchymal Stem Cells in Placental Syndromes: A Prospective Cohort Study |
| title_short | Reduced Proliferative Potential with Conserved Stem/Stromal Phenotype of Human Umbilical Cord Mesenchymal Stem Cells in Placental Syndromes: A Prospective Cohort Study |
| title_sort | reduced proliferative potential with conserved stem stromal phenotype of human umbilical cord mesenchymal stem cells in placental syndromes a prospective cohort study |
| topic | mesenchymal stem cells wharton's jelly cord blood placental syndromes intrauterine growth restriction |
| url | https://www.imrpress.com/journal/CEOG/50/9/10.31083/j.ceog5009196 |
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