Transcriptomic Analysis of Biofilm Formation Inhibition by PDIA Iminosugar in <i>Staphylococcus aureus</i>
<b>Background:</b> Iminosugars are natural or synthetic sugar analogues with a very broad spectrum of activities, including those against the most prominent bacterial pathogens, like <i>P. aeruginosa</i> or <i>S. aureus.</i> In a series of studies, we have demonst...
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MDPI AG
2025-07-01
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| author | Anna Tomusiak-Plebanek Łucja Kozień Estelle Gallienne Maciej Florczyk Sławomir Ciesielski Piotr Heczko Magdalena Strus |
| author_facet | Anna Tomusiak-Plebanek Łucja Kozień Estelle Gallienne Maciej Florczyk Sławomir Ciesielski Piotr Heczko Magdalena Strus |
| author_sort | Anna Tomusiak-Plebanek |
| collection | DOAJ |
| description | <b>Background:</b> Iminosugars are natural or synthetic sugar analogues with a very broad spectrum of activities, including those against the most prominent bacterial pathogens, like <i>P. aeruginosa</i> or <i>S. aureus.</i> In a series of studies, we have demonstrated that one of the synthetic iminosugars, PDIA (beta-1-C-propyl-1,4-dideoxy-1,4-imino-L-arabinitol), possesses the ability to suppress biofilm production by different pathogenic bacteria without inhibiting their growth. Thereby, PDIA is able to influence experimental skin infection caused by <i>S. aureus</i>. <b>Methods:</b> To elucidate molecular mechanisms by which PDIA impedes biofilm formation by <i>S. aureus</i>, a transcriptomic study was performed in which a biofilm-producing <i>S. aureus</i> strain was grown in the presence of PDIA for 24 and 48 h in comparison to a control without the iminosugar. The RNA was then isolated, converted into cDNA, sequenced, and data analysis was performed. <b>Results:</b> It appeared that PDIA caused the down-regulation of many bacteriophage genes responsible for the processes of bacterial cell lysis, and some genes responsible for cell wall degradation were also down-regulated. Among the 25 most upregulated genes were those representing the phosphotransferase system (PTS), which is required for carbohydrate uptake and control of carbon metabolism. The ranking of the most significant down-regulated genes after 24 h exposure to PDIA shows that they predominantly coded for both the synthesis and lysis of the peptidoglycan. <b>Conclusions:</b> We have shown here that the influence of PDIA on the expression of <i>S. aureus</i> genes is broad and affects many genes encoding metabolism and ribosomes. |
| format | Article |
| id | doaj-art-7fcdc3ffe883433a850af78cd71fb286 |
| institution | DOAJ |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-7fcdc3ffe883433a850af78cd71fb2862025-08-20T02:45:52ZengMDPI AGAntibiotics2079-63822025-07-0114766810.3390/antibiotics14070668Transcriptomic Analysis of Biofilm Formation Inhibition by PDIA Iminosugar in <i>Staphylococcus aureus</i>Anna Tomusiak-Plebanek0Łucja Kozień1Estelle Gallienne2Maciej Florczyk3Sławomir Ciesielski4Piotr Heczko5Magdalena Strus6Chair of Microbiology, Department of Bacteriology and Parasitology, Jagiellonian University Medical College, 31-121 Krakow, PolandChair of Microbiology, Department of Bacteriology and Parasitology, Jagiellonian University Medical College, 31-121 Krakow, PolandInstitut de Chimie Organique et Analytique (ICOA), UMR 7311, Université d’Orléans & CNRS, 45067 Orleans, FranceLaboratory of Molecular Neurobiology, Nencki Institute of Experimental Biology of the Polish Academy of Sciences, 02-093 Warsaw, PolandDepartment of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, 10-907 Olsztyn, PolandChair of Microbiology, Department of Bacteriology and Parasitology, Jagiellonian University Medical College, 31-121 Krakow, PolandChair of Microbiology, Department of Bacteriology and Parasitology, Jagiellonian University Medical College, 31-121 Krakow, Poland<b>Background:</b> Iminosugars are natural or synthetic sugar analogues with a very broad spectrum of activities, including those against the most prominent bacterial pathogens, like <i>P. aeruginosa</i> or <i>S. aureus.</i> In a series of studies, we have demonstrated that one of the synthetic iminosugars, PDIA (beta-1-C-propyl-1,4-dideoxy-1,4-imino-L-arabinitol), possesses the ability to suppress biofilm production by different pathogenic bacteria without inhibiting their growth. Thereby, PDIA is able to influence experimental skin infection caused by <i>S. aureus</i>. <b>Methods:</b> To elucidate molecular mechanisms by which PDIA impedes biofilm formation by <i>S. aureus</i>, a transcriptomic study was performed in which a biofilm-producing <i>S. aureus</i> strain was grown in the presence of PDIA for 24 and 48 h in comparison to a control without the iminosugar. The RNA was then isolated, converted into cDNA, sequenced, and data analysis was performed. <b>Results:</b> It appeared that PDIA caused the down-regulation of many bacteriophage genes responsible for the processes of bacterial cell lysis, and some genes responsible for cell wall degradation were also down-regulated. Among the 25 most upregulated genes were those representing the phosphotransferase system (PTS), which is required for carbohydrate uptake and control of carbon metabolism. The ranking of the most significant down-regulated genes after 24 h exposure to PDIA shows that they predominantly coded for both the synthesis and lysis of the peptidoglycan. <b>Conclusions:</b> We have shown here that the influence of PDIA on the expression of <i>S. aureus</i> genes is broad and affects many genes encoding metabolism and ribosomes.https://www.mdpi.com/2079-6382/14/7/668biofilmiminosugar PDIA<i>Staphylococcus aureus</i>transcriptomic analysis |
| spellingShingle | Anna Tomusiak-Plebanek Łucja Kozień Estelle Gallienne Maciej Florczyk Sławomir Ciesielski Piotr Heczko Magdalena Strus Transcriptomic Analysis of Biofilm Formation Inhibition by PDIA Iminosugar in <i>Staphylococcus aureus</i> Antibiotics biofilm iminosugar PDIA <i>Staphylococcus aureus</i> transcriptomic analysis |
| title | Transcriptomic Analysis of Biofilm Formation Inhibition by PDIA Iminosugar in <i>Staphylococcus aureus</i> |
| title_full | Transcriptomic Analysis of Biofilm Formation Inhibition by PDIA Iminosugar in <i>Staphylococcus aureus</i> |
| title_fullStr | Transcriptomic Analysis of Biofilm Formation Inhibition by PDIA Iminosugar in <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Transcriptomic Analysis of Biofilm Formation Inhibition by PDIA Iminosugar in <i>Staphylococcus aureus</i> |
| title_short | Transcriptomic Analysis of Biofilm Formation Inhibition by PDIA Iminosugar in <i>Staphylococcus aureus</i> |
| title_sort | transcriptomic analysis of biofilm formation inhibition by pdia iminosugar in i staphylococcus aureus i |
| topic | biofilm iminosugar PDIA <i>Staphylococcus aureus</i> transcriptomic analysis |
| url | https://www.mdpi.com/2079-6382/14/7/668 |
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