Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA
Brucellosis is a bacterial disease caused by various Brucella species, which mainly infect bovines (cattle, buffalo), small ruminants (sheep, goat), swine, and dogs and humans. Earlier studies have reported brucellosis sero-prevalence only in cattle or both in cattle and buffaloes (bovine brucellos...
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Indian Council of Agricultural Research
2024-12-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/142684 |
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| author | RAJESWARI SHOME SHARANGOUDA PATIL SAMER SHAMSHAD SOMY SKARIAH MUNEERA MOHAMED SAHIB G SHANMUGAM NAGENDRA NATH BARMAN DURLAV PRASAD BORA2 ARIJIT SHOME |
| author_facet | RAJESWARI SHOME SHARANGOUDA PATIL SAMER SHAMSHAD SOMY SKARIAH MUNEERA MOHAMED SAHIB G SHANMUGAM NAGENDRA NATH BARMAN DURLAV PRASAD BORA2 ARIJIT SHOME |
| author_sort | RAJESWARI SHOME |
| collection | DOAJ |
| description |
Brucellosis is a bacterial disease caused by various Brucella species, which mainly infect bovines (cattle, buffalo), small ruminants (sheep, goat), swine, and dogs and humans. Earlier studies have reported brucellosis sero-prevalence only in cattle or both in cattle and buffaloes (bovine brucellosis) and actual disease burden in buffaloes was not available. The current study aimed to record countrywide brucellosis sero-prevalence in Indian buffaloes using competitive ELISA (cELISA). For the study, 1086 female buffalo (Bubalus bubalis) serum samples were collected from the top 10 states of India having the highest buffalo population. Overall, samples were drawn from 62 districts, 104 blocks and 242 epiunits from five regions of the country ({Punjab-100, Haryana-109, Uttar Pradesh-101, Rajasthan-100, Gujarat-156, Andhra Pradesh-100, Madhya Pradesh-110, Maharashtra-108, Karnataka-102, Andhra Pradesh-100 and Tamil Nadu-100)}. Samples were tested for anti-brucella antibodies using in-house developed monoclonal-based competitive ELISA (cELISA). Overall, apparent prevalence (AP) of 15.38% (CI-95%;13.35-17.64) and true prevalence (TP) of 15.85% (CI-95%; 13.77-18.19) were recorded. Sero-prevalence was highest in Punjab state (AP:57%; 47.22-66.27) in the Northern region and lowest in Madhya Pradesh (AP:0.90; 0.16-4.97) state of Central region with some of the districts displaying up to 90% and 70%, seropositivity in the Punjab state. Low brucellosis prevalence was noted in young (2.1 to 5 years) and older age groups of animals (11.1 to 13 years) and significantly high in the age group between 8 to 11 years (32.23%). The highest brucellosis seropositivity was observed in buffalo breeds such as Nagpuri and Murrah (28.79% and 20.67%), respectively and significant association was noted among four breeds. In conclusion, India has the highest buffalo population with very high-yielding buffalo breeds (109.85 million). Periodical surveillance is essential to detect and control brucellosis in buffaloes.-the pride species of the country.
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| format | Article |
| id | doaj-art-7fccbf0fdff64ec1a08f299b5980908e |
| institution | DOAJ |
| issn | 0367-8318 2394-3327 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Indian Council of Agricultural Research |
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| series | Indian Journal of Animal Sciences |
| spelling | doaj-art-7fccbf0fdff64ec1a08f299b5980908e2025-08-20T02:52:15ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272024-12-01941210.56093/ijans.v94i12.142684Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISARAJESWARI SHOME0SHARANGOUDA PATIL1SAMER SHAMSHAD2SOMY SKARIAH3MUNEERA MOHAMED SAHIB4G SHANMUGAM5NAGENDRA NATH BARMAN6DURLAV PRASAD BORA27ARIJIT SHOME8ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 IndiaCollege of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, AssamCollege of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, AssamCollege of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam Brucellosis is a bacterial disease caused by various Brucella species, which mainly infect bovines (cattle, buffalo), small ruminants (sheep, goat), swine, and dogs and humans. Earlier studies have reported brucellosis sero-prevalence only in cattle or both in cattle and buffaloes (bovine brucellosis) and actual disease burden in buffaloes was not available. The current study aimed to record countrywide brucellosis sero-prevalence in Indian buffaloes using competitive ELISA (cELISA). For the study, 1086 female buffalo (Bubalus bubalis) serum samples were collected from the top 10 states of India having the highest buffalo population. Overall, samples were drawn from 62 districts, 104 blocks and 242 epiunits from five regions of the country ({Punjab-100, Haryana-109, Uttar Pradesh-101, Rajasthan-100, Gujarat-156, Andhra Pradesh-100, Madhya Pradesh-110, Maharashtra-108, Karnataka-102, Andhra Pradesh-100 and Tamil Nadu-100)}. Samples were tested for anti-brucella antibodies using in-house developed monoclonal-based competitive ELISA (cELISA). Overall, apparent prevalence (AP) of 15.38% (CI-95%;13.35-17.64) and true prevalence (TP) of 15.85% (CI-95%; 13.77-18.19) were recorded. Sero-prevalence was highest in Punjab state (AP:57%; 47.22-66.27) in the Northern region and lowest in Madhya Pradesh (AP:0.90; 0.16-4.97) state of Central region with some of the districts displaying up to 90% and 70%, seropositivity in the Punjab state. Low brucellosis prevalence was noted in young (2.1 to 5 years) and older age groups of animals (11.1 to 13 years) and significantly high in the age group between 8 to 11 years (32.23%). The highest brucellosis seropositivity was observed in buffalo breeds such as Nagpuri and Murrah (28.79% and 20.67%), respectively and significant association was noted among four breeds. In conclusion, India has the highest buffalo population with very high-yielding buffalo breeds (109.85 million). Periodical surveillance is essential to detect and control brucellosis in buffaloes.-the pride species of the country. https://epubs.icar.org.in/index.php/IJAnS/article/view/142684Bubalus bubalis, Brucellosis, cELISA, India, Sero-prevalence |
| spellingShingle | RAJESWARI SHOME SHARANGOUDA PATIL SAMER SHAMSHAD SOMY SKARIAH MUNEERA MOHAMED SAHIB G SHANMUGAM NAGENDRA NATH BARMAN DURLAV PRASAD BORA2 ARIJIT SHOME Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA Indian Journal of Animal Sciences Bubalus bubalis, Brucellosis, cELISA, India, Sero-prevalence |
| title | Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA |
| title_full | Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA |
| title_fullStr | Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA |
| title_full_unstemmed | Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA |
| title_short | Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA |
| title_sort | nationwide cross sectional study on brucellosis sero prevalence in buffaloes using competitive elisa |
| topic | Bubalus bubalis, Brucellosis, cELISA, India, Sero-prevalence |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/142684 |
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