Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in Mice
Mounting evidence indicates that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs-exosomes) combine the advantages of hucMSC pluripotency with their nanoscale dimensions, enhancing their clinical potential through prolonged circulation half-life. Despite these promising cha...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/sci/9986368 |
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| _version_ | 1850197502621384704 |
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| author | Cancan Wang Xinmei Hu Yu Liu Yu Xiao Peng Jiang Yunjing Lin Xiaomin Liu Zhengmian Zhang Liang-cheng Li Zhongquan Qi |
| author_facet | Cancan Wang Xinmei Hu Yu Liu Yu Xiao Peng Jiang Yunjing Lin Xiaomin Liu Zhengmian Zhang Liang-cheng Li Zhongquan Qi |
| author_sort | Cancan Wang |
| collection | DOAJ |
| description | Mounting evidence indicates that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs-exosomes) combine the advantages of hucMSC pluripotency with their nanoscale dimensions, enhancing their clinical potential through prolonged circulation half-life. Despite these promising characteristics, research on their immunological toxicity remains insufficient. This study focuses on the impact of hucMSC-exosomes on the general toxicity and immunopathological indicators. When mice received tail vein injections of 6 × 1010 hucMSC-exosomes particles, we observed no significant changes in body weight, feed intake, blood composition, organ indices, or histopathological findings throughout the 14 days observation period. Similarly, blood levels of immunoglobulins, cytokines, and lymphocyte subpopulations remained stable. The hucMSC-exosomes produced no detectable negative effects on immune organs including the thymus, spleen, and bone marrow. These findings indicate that intravenous administration of 6 × 1010 particles of hucMSC-exosomes appears relatively safe at the murine level. This assessment of safety and immunological impact following intravenous hucMSC-exosomes infusion offers experimental support for potential clinical applications and future analyses in this field. |
| format | Article |
| id | doaj-art-7fc758058e474d77a42df8a081313ef0 |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-7fc758058e474d77a42df8a081313ef02025-08-20T02:13:07ZengWileyStem Cells International1687-96782025-01-01202510.1155/sci/9986368Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in MiceCancan Wang0Xinmei Hu1Yu Liu2Yu Xiao3Peng Jiang4Yunjing Lin5Xiaomin Liu6Zhengmian Zhang7Liang-cheng Li8Zhongquan Qi9Guangxi Key Laboratory of Special BiomedicineGuangxi Key Laboratory of Special BiomedicineGuangxi Key Laboratory of Special BiomedicineGuangxi Key Laboratory of Special BiomedicineGuangxi Key Laboratory of Special BiomedicineGuangxi Key Laboratory of Special BiomedicineGuangxi Key Laboratory of Special BiomedicineFujian Provincial Sperm BankSchool of Pharmaceutical SciencesGuangxi Key Laboratory of Special BiomedicineMounting evidence indicates that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs-exosomes) combine the advantages of hucMSC pluripotency with their nanoscale dimensions, enhancing their clinical potential through prolonged circulation half-life. Despite these promising characteristics, research on their immunological toxicity remains insufficient. This study focuses on the impact of hucMSC-exosomes on the general toxicity and immunopathological indicators. When mice received tail vein injections of 6 × 1010 hucMSC-exosomes particles, we observed no significant changes in body weight, feed intake, blood composition, organ indices, or histopathological findings throughout the 14 days observation period. Similarly, blood levels of immunoglobulins, cytokines, and lymphocyte subpopulations remained stable. The hucMSC-exosomes produced no detectable negative effects on immune organs including the thymus, spleen, and bone marrow. These findings indicate that intravenous administration of 6 × 1010 particles of hucMSC-exosomes appears relatively safe at the murine level. This assessment of safety and immunological impact following intravenous hucMSC-exosomes infusion offers experimental support for potential clinical applications and future analyses in this field.http://dx.doi.org/10.1155/sci/9986368 |
| spellingShingle | Cancan Wang Xinmei Hu Yu Liu Yu Xiao Peng Jiang Yunjing Lin Xiaomin Liu Zhengmian Zhang Liang-cheng Li Zhongquan Qi Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in Mice Stem Cells International |
| title | Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in Mice |
| title_full | Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in Mice |
| title_fullStr | Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in Mice |
| title_full_unstemmed | Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in Mice |
| title_short | Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in Mice |
| title_sort | immunological safety evaluation of exosomes derived from human umbilical cord mesenchymal stem cells in mice |
| url | http://dx.doi.org/10.1155/sci/9986368 |
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