An Investigation of Electrospun Clerodendrum phlomidis Leaves Extract Infused Polycaprolactone Nanofiber for In Vitro Biological Application

The in vitro antibacterial, anticancer, and antioxidant activities of a few plant extracts were widely known for decades, and they were used for application in the conventional way. Specifically, electrospun nanofibrous mats have recently exhibited great antibacterial, anticancer, and antioxidant ac...

Full description

Saved in:
Bibliographic Details
Main Authors: Siranjeevi Ravichandran, Rajesh Jegathaprathaban, Jeyalakshmi Radhakrishnan, R. Usha, V. Vijayan, Aklilu Teklemariam
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Bioinorganic Chemistry and Applications
Online Access:http://dx.doi.org/10.1155/2022/2335443
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832558394598227968
author Siranjeevi Ravichandran
Rajesh Jegathaprathaban
Jeyalakshmi Radhakrishnan
R. Usha
V. Vijayan
Aklilu Teklemariam
author_facet Siranjeevi Ravichandran
Rajesh Jegathaprathaban
Jeyalakshmi Radhakrishnan
R. Usha
V. Vijayan
Aklilu Teklemariam
author_sort Siranjeevi Ravichandran
collection DOAJ
description The in vitro antibacterial, anticancer, and antioxidant activities of a few plant extracts were widely known for decades, and they were used for application in the conventional way. Specifically, electrospun nanofibrous mats have recently exhibited great antibacterial, anticancer, and antioxidant activities. The herbal extracts infused into these formations are expected to have a more efficient and integrated effect on in vitro biological applications. The purpose of this study is to develop polycaprolactone- (PCL-) based nanofiber mats that are infused with a traditional plant extract using Clerodendrum phlomidis leaves to improve the synthesized nanofibers’ antibacterial, anticancer, and antioxidant efficacy. This study examined the morphology, thermal properties, mechanical properties, structure, and in vitro drug release studies of electrospun nanofibers. Antibacterial, anticancer, and antioxidant activities of the electrospun nanofibrous mats were also studied. The HRTEM and FESEM pictures of PCL and PCL-CPM nanofibers provide that smooth, defect-free, and homogeneous nanofibers were found to be 602.08 ± 75 nm and 414.15 ± 82 nm for PCL and PCL-CPM nanofibers, respectively. The presence of Clerodendrum phlomidis extract in the electrospun nanofibers was approved by UV-visible and FTIR spectroscopy. The incorporation of Clerodendrum phlomidis extract to nanofiber mats resulted in substantial antibacterial activity against bacterial cells. PCL-CPM mats exposed to oral cancer (HSC-3) and renal cell carcinoma (ACHN) cell lines displayed promising anticancer activity with less than 50% survival rate after 24 h of incubation. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay performed on PCL-CPM nanofibers revealed the antioxidant scavenging activity with maximum inhibition of 34% suggesting the role of the secondary metabolites release from scaffold. As a result, the findings of this study revealed that Clerodendrum phlomidis extract encapsulating PCL electrospun nanofibers has a high potential for usage as a biobased antibacterial, anticancer, and antioxidant agent.
format Article
id doaj-art-7fc0dc3e2caa4aad8b83630310e6f900
institution Kabale University
issn 1687-479X
language English
publishDate 2022-01-01
publisher Wiley
record_format Article
series Bioinorganic Chemistry and Applications
spelling doaj-art-7fc0dc3e2caa4aad8b83630310e6f9002025-02-03T01:32:32ZengWileyBioinorganic Chemistry and Applications1687-479X2022-01-01202210.1155/2022/2335443An Investigation of Electrospun Clerodendrum phlomidis Leaves Extract Infused Polycaprolactone Nanofiber for In Vitro Biological ApplicationSiranjeevi Ravichandran0Rajesh Jegathaprathaban1Jeyalakshmi Radhakrishnan2R. Usha3V. Vijayan4Aklilu Teklemariam5Department of ChemistryDepartment of ChemistryDepartment of ChemistryDepartment of PhysicsDepartment of Mechanical EngineeringDepartment of Mechanical EngineeringThe in vitro antibacterial, anticancer, and antioxidant activities of a few plant extracts were widely known for decades, and they were used for application in the conventional way. Specifically, electrospun nanofibrous mats have recently exhibited great antibacterial, anticancer, and antioxidant activities. The herbal extracts infused into these formations are expected to have a more efficient and integrated effect on in vitro biological applications. The purpose of this study is to develop polycaprolactone- (PCL-) based nanofiber mats that are infused with a traditional plant extract using Clerodendrum phlomidis leaves to improve the synthesized nanofibers’ antibacterial, anticancer, and antioxidant efficacy. This study examined the morphology, thermal properties, mechanical properties, structure, and in vitro drug release studies of electrospun nanofibers. Antibacterial, anticancer, and antioxidant activities of the electrospun nanofibrous mats were also studied. The HRTEM and FESEM pictures of PCL and PCL-CPM nanofibers provide that smooth, defect-free, and homogeneous nanofibers were found to be 602.08 ± 75 nm and 414.15 ± 82 nm for PCL and PCL-CPM nanofibers, respectively. The presence of Clerodendrum phlomidis extract in the electrospun nanofibers was approved by UV-visible and FTIR spectroscopy. The incorporation of Clerodendrum phlomidis extract to nanofiber mats resulted in substantial antibacterial activity against bacterial cells. PCL-CPM mats exposed to oral cancer (HSC-3) and renal cell carcinoma (ACHN) cell lines displayed promising anticancer activity with less than 50% survival rate after 24 h of incubation. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay performed on PCL-CPM nanofibers revealed the antioxidant scavenging activity with maximum inhibition of 34% suggesting the role of the secondary metabolites release from scaffold. As a result, the findings of this study revealed that Clerodendrum phlomidis extract encapsulating PCL electrospun nanofibers has a high potential for usage as a biobased antibacterial, anticancer, and antioxidant agent.http://dx.doi.org/10.1155/2022/2335443
spellingShingle Siranjeevi Ravichandran
Rajesh Jegathaprathaban
Jeyalakshmi Radhakrishnan
R. Usha
V. Vijayan
Aklilu Teklemariam
An Investigation of Electrospun Clerodendrum phlomidis Leaves Extract Infused Polycaprolactone Nanofiber for In Vitro Biological Application
Bioinorganic Chemistry and Applications
title An Investigation of Electrospun Clerodendrum phlomidis Leaves Extract Infused Polycaprolactone Nanofiber for In Vitro Biological Application
title_full An Investigation of Electrospun Clerodendrum phlomidis Leaves Extract Infused Polycaprolactone Nanofiber for In Vitro Biological Application
title_fullStr An Investigation of Electrospun Clerodendrum phlomidis Leaves Extract Infused Polycaprolactone Nanofiber for In Vitro Biological Application
title_full_unstemmed An Investigation of Electrospun Clerodendrum phlomidis Leaves Extract Infused Polycaprolactone Nanofiber for In Vitro Biological Application
title_short An Investigation of Electrospun Clerodendrum phlomidis Leaves Extract Infused Polycaprolactone Nanofiber for In Vitro Biological Application
title_sort investigation of electrospun clerodendrum phlomidis leaves extract infused polycaprolactone nanofiber for in vitro biological application
url http://dx.doi.org/10.1155/2022/2335443
work_keys_str_mv AT siranjeeviravichandran aninvestigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT rajeshjegathaprathaban aninvestigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT jeyalakshmiradhakrishnan aninvestigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT rusha aninvestigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT vvijayan aninvestigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT akliluteklemariam aninvestigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT siranjeeviravichandran investigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT rajeshjegathaprathaban investigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT jeyalakshmiradhakrishnan investigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT rusha investigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT vvijayan investigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication
AT akliluteklemariam investigationofelectrospunclerodendrumphlomidisleavesextractinfusedpolycaprolactonenanofiberforinvitrobiologicalapplication