Lipofundin-Induced Hyperlipidemia Promotes Oxidative Stress and Atherosclerotic Lesions in New Zealand White Rabbits
Atherosclerosis represents a major cause of death in the world. It is known that Lipofundin 20% induces atherosclerotic lesions in rabbits, but its effects on serum lipids behaviour and redox environment have not been addressed. In this study, New Zealand rabbits were treated with 2 mL/kg of Lipofun...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/898769 |
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| author | Livan Delgado Roche Emilio Acosta Medina Ángela Fraga Pérez María A. Bécquer Viart Yosdel Soto López Viviana Falcón Cama Ana M. Vázquez López Gregorio Martínez-Sánchez Eduardo Fernández-Sánchez |
| author_facet | Livan Delgado Roche Emilio Acosta Medina Ángela Fraga Pérez María A. Bécquer Viart Yosdel Soto López Viviana Falcón Cama Ana M. Vázquez López Gregorio Martínez-Sánchez Eduardo Fernández-Sánchez |
| author_sort | Livan Delgado Roche |
| collection | DOAJ |
| description | Atherosclerosis represents a major cause of death in the world. It is known that Lipofundin 20% induces atherosclerotic lesions in rabbits, but its effects on serum lipids behaviour and redox environment have not been addressed. In this study, New Zealand rabbits were treated with 2 mL/kg of Lipofundin for 8 days. Then, redox biomarkers and serum lipids were determined spectrophotometrically. On the other hand, the development of atherosclerotic lesions was confirmed by eosin/hematoxylin staining and electron microscopy. At the end of the experiment, total cholesterol, triglycerides, cholesterol-LDL, and cholesterol-HDL levels were significantly increased. Also, a high index of biomolecules damage, a disruption of both enzymatic and nonenzymatic defenses, and a reduction of nitric oxide were observed. Our data demonstrated that Lipofundin 20% induces hyperlipidemia, which promotes an oxidative stress state. Due to the importance of these phenomena as risk factors for atherogenesis, we suggest that Lipofundin induces atherosclerosis mainly through these mechanisms. |
| format | Article |
| id | doaj-art-7f9b896f0dfc4c4ea54182a2c208a1e8 |
| institution | Kabale University |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-7f9b896f0dfc4c4ea54182a2c208a1e82025-02-03T05:51:47ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322012-01-01201210.1155/2012/898769898769Lipofundin-Induced Hyperlipidemia Promotes Oxidative Stress and Atherosclerotic Lesions in New Zealand White RabbitsLivan Delgado Roche0Emilio Acosta Medina1Ángela Fraga Pérez2María A. Bécquer Viart3Yosdel Soto López4Viviana Falcón Cama5Ana M. Vázquez López6Gregorio Martínez-Sánchez7Eduardo Fernández-Sánchez8Center of Studies for Research and Biological Evaluations, Pharmacy and Food Science College, University of Havana, PO. Box 13 600, La Coronela, La Lisa, Havana 13600, CubaCenter of Studies for Research and Biological Evaluations, Pharmacy and Food Science College, University of Havana, PO. Box 13 600, La Coronela, La Lisa, Havana 13600, CubaCenter of Studies for Research and Biological Evaluations, Pharmacy and Food Science College, University of Havana, PO. Box 13 600, La Coronela, La Lisa, Havana 13600, CubaCenter of Studies for Research and Biological Evaluations, Pharmacy and Food Science College, University of Havana, PO. Box 13 600, La Coronela, La Lisa, Havana 13600, CubaDepartment of Antibody Engineering, Center of Molecular Immunology, Havana 11600, CubaDepartment of Electron Microscopy, Center for Genetic Engineering and Biotechnology, Havana 10600, CubaDepartment of Antibody Engineering, Center of Molecular Immunology, Havana 11600, CubaMediNat, 60021 Ancona, ItalyCenter of Studies for Research and Biological Evaluations, Pharmacy and Food Science College, University of Havana, PO. Box 13 600, La Coronela, La Lisa, Havana 13600, CubaAtherosclerosis represents a major cause of death in the world. It is known that Lipofundin 20% induces atherosclerotic lesions in rabbits, but its effects on serum lipids behaviour and redox environment have not been addressed. In this study, New Zealand rabbits were treated with 2 mL/kg of Lipofundin for 8 days. Then, redox biomarkers and serum lipids were determined spectrophotometrically. On the other hand, the development of atherosclerotic lesions was confirmed by eosin/hematoxylin staining and electron microscopy. At the end of the experiment, total cholesterol, triglycerides, cholesterol-LDL, and cholesterol-HDL levels were significantly increased. Also, a high index of biomolecules damage, a disruption of both enzymatic and nonenzymatic defenses, and a reduction of nitric oxide were observed. Our data demonstrated that Lipofundin 20% induces hyperlipidemia, which promotes an oxidative stress state. Due to the importance of these phenomena as risk factors for atherogenesis, we suggest that Lipofundin induces atherosclerosis mainly through these mechanisms.http://dx.doi.org/10.1155/2012/898769 |
| spellingShingle | Livan Delgado Roche Emilio Acosta Medina Ángela Fraga Pérez María A. Bécquer Viart Yosdel Soto López Viviana Falcón Cama Ana M. Vázquez López Gregorio Martínez-Sánchez Eduardo Fernández-Sánchez Lipofundin-Induced Hyperlipidemia Promotes Oxidative Stress and Atherosclerotic Lesions in New Zealand White Rabbits International Journal of Vascular Medicine |
| title | Lipofundin-Induced Hyperlipidemia Promotes Oxidative Stress and Atherosclerotic Lesions in New Zealand White Rabbits |
| title_full | Lipofundin-Induced Hyperlipidemia Promotes Oxidative Stress and Atherosclerotic Lesions in New Zealand White Rabbits |
| title_fullStr | Lipofundin-Induced Hyperlipidemia Promotes Oxidative Stress and Atherosclerotic Lesions in New Zealand White Rabbits |
| title_full_unstemmed | Lipofundin-Induced Hyperlipidemia Promotes Oxidative Stress and Atherosclerotic Lesions in New Zealand White Rabbits |
| title_short | Lipofundin-Induced Hyperlipidemia Promotes Oxidative Stress and Atherosclerotic Lesions in New Zealand White Rabbits |
| title_sort | lipofundin induced hyperlipidemia promotes oxidative stress and atherosclerotic lesions in new zealand white rabbits |
| url | http://dx.doi.org/10.1155/2012/898769 |
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