The Expression and Regulatory Role of lncRNA CRNDE in Ischemic Stroke
Abstract Background Ischemic stroke (IS) is a disease caused by the occlusion of cerebral arteries with lack of oxygen and blood supply and is characterized by high morbidity and mortality. The role of lncRNA CRNDE in IS remains unclear. Objective This study focused on investigating the function of...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-04-01
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| Series: | Artery Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s44200-025-00079-7 |
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| Summary: | Abstract Background Ischemic stroke (IS) is a disease caused by the occlusion of cerebral arteries with lack of oxygen and blood supply and is characterized by high morbidity and mortality. The role of lncRNA CRNDE in IS remains unclear. Objective This study focused on investigating the function of lncRNA CRNDE in IS. Materials and Methods 237 participants were enrolled in this study, including 135 patients with ischemic stroke (Ischemic stroke group) and 102 healthy individuals (Control group). The human brain microvascular endothelial cells (BMECs) treated with oxygen–glucose deprivation (OGD) were used to establish an IS cell model in vitro. The qRT-PCR was used to analyze the expression level of lncRNA CRNDE, miR-451, and MIF. The cell proliferation ability and migration were detected by CCK-8 and Transwell assay, respectively, while cell apoptosis was determined by apoptosis assay kit and flow cytometry. Additionally, the target relationship of CRNDE/miR-451 and miR-451/MIF was verified by dual-luciferase system. Results LncRNA CRNDE exhibited an up-regulated expression in IS patients and the IS cell model. Down-regulated lncRNA CRNDE not only contributed to cell proliferation ability and migration but also inhibited cell apoptosis. The miR-451 was a potential target of lncRNA CRNDE. The increased MIF was covalently bound to miR-451 and MIF reduction caused by CRNDE silence was significantly rescued by the inhibition of miR-451. Conclusion Up-regulated lncRNA CRNDE exacerbated IS by regulating proliferation, migration, and apoptosis in IS cell model. MiR-451/MIF was a potential downstream target of lncRNA CRNDE. |
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| ISSN: | 1876-4401 |