Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more
The generation of myogenic progenitors from iPSCs (iMPs) with therapeutic potential for in vivo tissue regeneration has long been a goal in the skeletal muscle community. Today, protocols enable the production of potent, albeit immature, iMPs that resemble Pax7+ adult muscle stem cells. While muscul...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2024.1526635/full |
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| author | Mark Stephen Hamer Fabio M. V. Rossi |
| author_facet | Mark Stephen Hamer Fabio M. V. Rossi |
| author_sort | Mark Stephen Hamer |
| collection | DOAJ |
| description | The generation of myogenic progenitors from iPSCs (iMPs) with therapeutic potential for in vivo tissue regeneration has long been a goal in the skeletal muscle community. Today, protocols enable the production of potent, albeit immature, iMPs that resemble Pax7+ adult muscle stem cells. While muscular dystrophies are often the primary therapeutic target for these cells, an underexplored application is their use in treating traumatic muscle injuries. Notably absent from recent reviews on iMPs is the concept of engineering these cells to perform functions post-transplantation that non-transgenic cells cannot. Here, we highlight protocols to enhance the generation, purification, and maturation of iMPs, and introduce the idea of engineering these cells to perform functions beyond their normal capacities, envisioning novel therapeutic applications. |
| format | Article |
| id | doaj-art-7f8ecc47e72a45be9289b2def09c2e61 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-7f8ecc47e72a45be9289b2def09c2e612025-01-22T07:13:19ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011210.3389/fcell.2024.15266351526635Multitasking muscle: engineering iPSC-derived myogenic progenitors to do moreMark Stephen Hamer0Fabio M. V. Rossi1School of Biomedical Engineering, University of British Columbia, Vancouver, BC, CanadaDepartment of Medical Genetics, University of British Columbia, Vancouver, BC, CanadaThe generation of myogenic progenitors from iPSCs (iMPs) with therapeutic potential for in vivo tissue regeneration has long been a goal in the skeletal muscle community. Today, protocols enable the production of potent, albeit immature, iMPs that resemble Pax7+ adult muscle stem cells. While muscular dystrophies are often the primary therapeutic target for these cells, an underexplored application is their use in treating traumatic muscle injuries. Notably absent from recent reviews on iMPs is the concept of engineering these cells to perform functions post-transplantation that non-transgenic cells cannot. Here, we highlight protocols to enhance the generation, purification, and maturation of iMPs, and introduce the idea of engineering these cells to perform functions beyond their normal capacities, envisioning novel therapeutic applications.https://www.frontiersin.org/articles/10.3389/fcell.2024.1526635/fullmyogenic progenitor cellsmuscle regenerationcell therapeuticsynthetic biology (synbio)muscle stem cell (MuSC)iPSCs (induced pluripotent stem cells) |
| spellingShingle | Mark Stephen Hamer Fabio M. V. Rossi Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more Frontiers in Cell and Developmental Biology myogenic progenitor cells muscle regeneration cell therapeutic synthetic biology (synbio) muscle stem cell (MuSC) iPSCs (induced pluripotent stem cells) |
| title | Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more |
| title_full | Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more |
| title_fullStr | Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more |
| title_full_unstemmed | Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more |
| title_short | Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more |
| title_sort | multitasking muscle engineering ipsc derived myogenic progenitors to do more |
| topic | myogenic progenitor cells muscle regeneration cell therapeutic synthetic biology (synbio) muscle stem cell (MuSC) iPSCs (induced pluripotent stem cells) |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2024.1526635/full |
| work_keys_str_mv | AT markstephenhamer multitaskingmuscleengineeringipscderivedmyogenicprogenitorstodomore AT fabiomvrossi multitaskingmuscleengineeringipscderivedmyogenicprogenitorstodomore |