Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte
The Electrospray Ionization (ESI) is a soft ionization technique extensively used for production of gas phase ions (without fragmentation) of thermally labile large supramolecules. In the present review we have described the development of Electrospray Ionization mass spectrometry (ESI-MS) during th...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/282574 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850214992290250752 |
|---|---|
| author | Shibdas Banerjee Shyamalava Mazumdar |
| author_facet | Shibdas Banerjee Shyamalava Mazumdar |
| author_sort | Shibdas Banerjee |
| collection | DOAJ |
| description | The Electrospray Ionization (ESI) is a soft ionization technique extensively used for production of gas phase ions (without fragmentation) of thermally labile large supramolecules. In the present review we have described the development of Electrospray Ionization mass spectrometry (ESI-MS) during the last 25 years in the study of various properties of different types of biological molecules. There have been extensive studies on the mechanism of formation of charged gaseous species by the ESI. Several groups have investigated the origin and implications of the multiple charge states of proteins observed in the ESI-mass spectra of the proteins. The charged analytes produced by ESI can be fragmented by activating them in the gas-phase, and thus tandem mass spectrometry has been developed, which provides very important insights on the structural properties of the molecule. The review will highlight recent developments and emerging directions in this fascinating area of research. |
| format | Article |
| id | doaj-art-7f8b030606d14523b42ae67befdb91f6 |
| institution | OA Journals |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-7f8b030606d14523b42ae67befdb91f62025-08-20T02:08:45ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792012-01-01201210.1155/2012/282574282574Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the AnalyteShibdas Banerjee0Shyamalava Mazumdar1 Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, India Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, IndiaThe Electrospray Ionization (ESI) is a soft ionization technique extensively used for production of gas phase ions (without fragmentation) of thermally labile large supramolecules. In the present review we have described the development of Electrospray Ionization mass spectrometry (ESI-MS) during the last 25 years in the study of various properties of different types of biological molecules. There have been extensive studies on the mechanism of formation of charged gaseous species by the ESI. Several groups have investigated the origin and implications of the multiple charge states of proteins observed in the ESI-mass spectra of the proteins. The charged analytes produced by ESI can be fragmented by activating them in the gas-phase, and thus tandem mass spectrometry has been developed, which provides very important insights on the structural properties of the molecule. The review will highlight recent developments and emerging directions in this fascinating area of research.http://dx.doi.org/10.1155/2012/282574 |
| spellingShingle | Shibdas Banerjee Shyamalava Mazumdar Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte International Journal of Analytical Chemistry |
| title | Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte |
| title_full | Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte |
| title_fullStr | Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte |
| title_full_unstemmed | Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte |
| title_short | Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte |
| title_sort | electrospray ionization mass spectrometry a technique to access the information beyond the molecular weight of the analyte |
| url | http://dx.doi.org/10.1155/2012/282574 |
| work_keys_str_mv | AT shibdasbanerjee electrosprayionizationmassspectrometryatechniquetoaccesstheinformationbeyondthemolecularweightoftheanalyte AT shyamalavamazumdar electrosprayionizationmassspectrometryatechniquetoaccesstheinformationbeyondthemolecularweightoftheanalyte |