A bacterial host factor confines phage localization for excluding the infected compartment through cell division
Summary: Viruses frequently induce the formation of specialized subcellular compartments to facilitate their replication and assembly. Here, we describe a “host-derived” confinement mechanism, compartmentalizing bacteriophage (phage) production to enable phage caging through cell division. By employ...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112472500765X |
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| author | Osher Pollak Fiyaksel Somavally Pundalik Dalvi Bing Zhou Miriam Ravins Bushra Shraiteh Saurabh Bhattacharya Saveliy Kirillov Prabhjot Kaur Ilan Rosenshine Debnath Ghosal Sigal Ben-Yehuda |
| author_facet | Osher Pollak Fiyaksel Somavally Pundalik Dalvi Bing Zhou Miriam Ravins Bushra Shraiteh Saurabh Bhattacharya Saveliy Kirillov Prabhjot Kaur Ilan Rosenshine Debnath Ghosal Sigal Ben-Yehuda |
| author_sort | Osher Pollak Fiyaksel |
| collection | DOAJ |
| description | Summary: Viruses frequently induce the formation of specialized subcellular compartments to facilitate their replication and assembly. Here, we describe a “host-derived” confinement mechanism, compartmentalizing bacteriophage (phage) production to enable phage caging through cell division. By employing the bacterium Bacillus subtilis and its lytic phages, we identified YjbH, highly conserved among gram-positive bacteria, as a host factor that limits plaque expansion. YjbH directly binds the penetrating phage genome via its helix-turn-helix DNA-binding domain and accumulates into a focus at the site of DNA injection. YjbH further constricts the synthesis of phage components, including DNA and capsid proteins, to a specific subcellular locale. Consequently, the division machinery is recruited to produce adjacent septations, often asymmetric, effectively trapping and excluding the infected compartment. This “exclude and survive” defense mechanism may represent a prevalent strategy employed by the host to contain viral spread. |
| format | Article |
| id | doaj-art-7f8743e532254c25bcfe82e7baffe0a6 |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-7f8743e532254c25bcfe82e7baffe0a62025-08-20T03:28:38ZengElsevierCell Reports2211-12472025-07-0144711599410.1016/j.celrep.2025.115994A bacterial host factor confines phage localization for excluding the infected compartment through cell divisionOsher Pollak Fiyaksel0Somavally Pundalik Dalvi1Bing Zhou2Miriam Ravins3Bushra Shraiteh4Saurabh Bhattacharya5Saveliy Kirillov6Prabhjot Kaur7Ilan Rosenshine8Debnath Ghosal9Sigal Ben-Yehuda10Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, IsraelDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia; ARC Centre for Cryo-electron Microscopy of Membrane Proteins, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, AustraliaDepartment of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, IsraelDepartment of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, IsraelDepartment of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, IsraelDepartment of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, IsraelDepartment of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel; National Center for Biotechnology, Astana 010000, KazakhstanDepartment of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, IsraelDepartment of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, IsraelDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia; ARC Centre for Cryo-electron Microscopy of Membrane Proteins, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, Australia; Corresponding authorDepartment of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University-Hadassah Medical School, Post Office Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel; Corresponding authorSummary: Viruses frequently induce the formation of specialized subcellular compartments to facilitate their replication and assembly. Here, we describe a “host-derived” confinement mechanism, compartmentalizing bacteriophage (phage) production to enable phage caging through cell division. By employing the bacterium Bacillus subtilis and its lytic phages, we identified YjbH, highly conserved among gram-positive bacteria, as a host factor that limits plaque expansion. YjbH directly binds the penetrating phage genome via its helix-turn-helix DNA-binding domain and accumulates into a focus at the site of DNA injection. YjbH further constricts the synthesis of phage components, including DNA and capsid proteins, to a specific subcellular locale. Consequently, the division machinery is recruited to produce adjacent septations, often asymmetric, effectively trapping and excluding the infected compartment. This “exclude and survive” defense mechanism may represent a prevalent strategy employed by the host to contain viral spread.http://www.sciencedirect.com/science/article/pii/S221112472500765XCP: Microbiology |
| spellingShingle | Osher Pollak Fiyaksel Somavally Pundalik Dalvi Bing Zhou Miriam Ravins Bushra Shraiteh Saurabh Bhattacharya Saveliy Kirillov Prabhjot Kaur Ilan Rosenshine Debnath Ghosal Sigal Ben-Yehuda A bacterial host factor confines phage localization for excluding the infected compartment through cell division Cell Reports CP: Microbiology |
| title | A bacterial host factor confines phage localization for excluding the infected compartment through cell division |
| title_full | A bacterial host factor confines phage localization for excluding the infected compartment through cell division |
| title_fullStr | A bacterial host factor confines phage localization for excluding the infected compartment through cell division |
| title_full_unstemmed | A bacterial host factor confines phage localization for excluding the infected compartment through cell division |
| title_short | A bacterial host factor confines phage localization for excluding the infected compartment through cell division |
| title_sort | bacterial host factor confines phage localization for excluding the infected compartment through cell division |
| topic | CP: Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S221112472500765X |
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