Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro
Multipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues. In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal cap...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2012/752083 |
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| author | A. A. Ismail S. Wagner H. Murua Escobar S. Willenbrock K. A. Sterenczak M. T. Samy A. M. Abd El-Aal I. Nolte P. Wefstaedt |
| author_facet | A. A. Ismail S. Wagner H. Murua Escobar S. Willenbrock K. A. Sterenczak M. T. Samy A. M. Abd El-Aal I. Nolte P. Wefstaedt |
| author_sort | A. A. Ismail |
| collection | DOAJ |
| description | Multipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues. In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal capacities has not yet been investigated. Therefore, extracellular HMGA1 and HMGA2 application alone (10–200 ng/mL) and in combination with each other (100, 200 ng/mL each) was investigated with regard to proliferative effects on canine ASCs (cASCs) after 48 hours of cultivation. Furthermore, mRNA expression of multipotency marker genes in unstimulated and HMGA2-stimulated cASCs (50, 100 ng/mL) was analyzed by RT-qPCR. HMGA1 significantly reduced cASCs proliferation in concentrations of 10–200 ng/mL culture medium. A combination of HMGA1 and HMGA2 protein (100 and 200 ng/mL each) caused the same effects, whereas no significant effect on cASCs proliferation was shown after HMGA2 protein application alone. RT-qPCR results showed that expression levels of marker genes including KLF4, SOX2, OCT4, HMGA2, and cMYC mRNAs were on the same level in both HMGA2-protein-stimulated and -unstimulated cASCs. Extracellular HMGA protein application might be valuable to control proliferation of cASCs in context with their employment in regenerative approaches without affecting their self-renewal and multipotency abilities. |
| format | Article |
| id | doaj-art-7f8176b210ef407796ffa5d1c06b9e30 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-7f8176b210ef407796ffa5d1c06b9e302025-08-20T03:35:20ZengWileyVeterinary Medicine International2090-81132042-00482012-01-01201210.1155/2012/752083752083Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In VitroA. A. Ismail0S. Wagner1H. Murua Escobar2S. Willenbrock3K. A. Sterenczak4M. T. Samy5A. M. Abd El-Aal6I. Nolte7P. Wefstaedt8Small Animal Hospital, University of Veterinary Medicine Foundation, 30559 Hannover, GermanySmall Animal Hospital, University of Veterinary Medicine Foundation, 30559 Hannover, GermanySmall Animal Hospital, University of Veterinary Medicine Foundation, 30559 Hannover, GermanySmall Animal Hospital, University of Veterinary Medicine Foundation, 30559 Hannover, GermanySmall Animal Hospital, University of Veterinary Medicine Foundation, 30559 Hannover, GermanyDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University, El-Sharkia, EgyptDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University, El-Sharkia, EgyptSmall Animal Hospital, University of Veterinary Medicine Foundation, 30559 Hannover, GermanySmall Animal Hospital, University of Veterinary Medicine Foundation, 30559 Hannover, GermanyMultipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues. In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal capacities has not yet been investigated. Therefore, extracellular HMGA1 and HMGA2 application alone (10–200 ng/mL) and in combination with each other (100, 200 ng/mL each) was investigated with regard to proliferative effects on canine ASCs (cASCs) after 48 hours of cultivation. Furthermore, mRNA expression of multipotency marker genes in unstimulated and HMGA2-stimulated cASCs (50, 100 ng/mL) was analyzed by RT-qPCR. HMGA1 significantly reduced cASCs proliferation in concentrations of 10–200 ng/mL culture medium. A combination of HMGA1 and HMGA2 protein (100 and 200 ng/mL each) caused the same effects, whereas no significant effect on cASCs proliferation was shown after HMGA2 protein application alone. RT-qPCR results showed that expression levels of marker genes including KLF4, SOX2, OCT4, HMGA2, and cMYC mRNAs were on the same level in both HMGA2-protein-stimulated and -unstimulated cASCs. Extracellular HMGA protein application might be valuable to control proliferation of cASCs in context with their employment in regenerative approaches without affecting their self-renewal and multipotency abilities.http://dx.doi.org/10.1155/2012/752083 |
| spellingShingle | A. A. Ismail S. Wagner H. Murua Escobar S. Willenbrock K. A. Sterenczak M. T. Samy A. M. Abd El-Aal I. Nolte P. Wefstaedt Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro Veterinary Medicine International |
| title | Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro |
| title_full | Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro |
| title_fullStr | Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro |
| title_full_unstemmed | Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro |
| title_short | Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro |
| title_sort | effects of high mobility group a protein application on canine adipose derived mesenchymal stem cells in vitro |
| url | http://dx.doi.org/10.1155/2012/752083 |
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