Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healing
Glucose oxidase (GOD) is a well-known flavin adenine dinucleotide (FAD)-containing oxidase. GOD oxidizes glucose to D-glucono-δ-lactone with FAD being reduced to FADH2, and O2 is reduced by FADH2 to hydrogen peroxide (H2O2). To efficiently generate H2O2 and subsequently produce radicals through gluc...
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Elsevier
2025-06-01
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| author | Yining Han Jiaxing Wei Wei Feng |
| author_facet | Yining Han Jiaxing Wei Wei Feng |
| author_sort | Yining Han |
| collection | DOAJ |
| description | Glucose oxidase (GOD) is a well-known flavin adenine dinucleotide (FAD)-containing oxidase. GOD oxidizes glucose to D-glucono-δ-lactone with FAD being reduced to FADH2, and O2 is reduced by FADH2 to hydrogen peroxide (H2O2). To efficiently generate H2O2 and subsequently produce radicals through glucose consumption, we synthesized the hybrid material GOD@Cu&Ce by encapsulating GOD with CuII and CeIV ions in phosphate buffer saline (PBS). Owing to the synergistic cooperation between CuII and CeIV, hydroxyl (•OH), hydroperoxyl (HOO•), and superoxide (O2•-) radicals are efficiently generated via H2O2 decomposition by Cu&Ce. Concurrently, O2 is produced, demonstrating that Cu&Ce exhibits catalase-like activity. The in situ-generated O2 facilitates the oxidation of FADH2 back to FAD, thereby enhancing GOD activity. The efficient and sustained generation of •OH, HOO•, and O2•- radicals from glucose conversion arises from two mechanisms: (1) synergistic H2O2 decomposition by enzyme-mimicking Cu&Ce, and (2) in situ O2 production that boosts GOD activity. Studies on the mechanisms reveal that CuII and CeIV cooperatively promote the generation of •OH, HOO•, O2•-, and O2. GOD@Cu&Ce combines rapid glucose consumption with high radical yield. In wound healing assays, this hybrid material demonstrates potent antibacterial activity. |
| format | Article |
| id | doaj-art-7f7b0aa08994449780889a42933939c1 |
| institution | OA Journals |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| spelling | doaj-art-7f7b0aa08994449780889a42933939c12025-08-20T01:52:55ZengElsevierMaterials Today Bio2590-00642025-06-013210188810.1016/j.mtbio.2025.101888Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healingYining Han0Jiaxing Wei1Wei Feng2Department of Biological Engineering, Beijing University of Chemical Technology, Beijing, ChinaDepartment of Biological Engineering, Beijing University of Chemical Technology, Beijing, ChinaCorresponding author.; Department of Biological Engineering, Beijing University of Chemical Technology, Beijing, ChinaGlucose oxidase (GOD) is a well-known flavin adenine dinucleotide (FAD)-containing oxidase. GOD oxidizes glucose to D-glucono-δ-lactone with FAD being reduced to FADH2, and O2 is reduced by FADH2 to hydrogen peroxide (H2O2). To efficiently generate H2O2 and subsequently produce radicals through glucose consumption, we synthesized the hybrid material GOD@Cu&Ce by encapsulating GOD with CuII and CeIV ions in phosphate buffer saline (PBS). Owing to the synergistic cooperation between CuII and CeIV, hydroxyl (•OH), hydroperoxyl (HOO•), and superoxide (O2•-) radicals are efficiently generated via H2O2 decomposition by Cu&Ce. Concurrently, O2 is produced, demonstrating that Cu&Ce exhibits catalase-like activity. The in situ-generated O2 facilitates the oxidation of FADH2 back to FAD, thereby enhancing GOD activity. The efficient and sustained generation of •OH, HOO•, and O2•- radicals from glucose conversion arises from two mechanisms: (1) synergistic H2O2 decomposition by enzyme-mimicking Cu&Ce, and (2) in situ O2 production that boosts GOD activity. Studies on the mechanisms reveal that CuII and CeIV cooperatively promote the generation of •OH, HOO•, O2•-, and O2. GOD@Cu&Ce combines rapid glucose consumption with high radical yield. In wound healing assays, this hybrid material demonstrates potent antibacterial activity.http://www.sciencedirect.com/science/article/pii/S259000642500448XGlucose oxidaseRadicalsCerium ionCopper ionWound healing |
| spellingShingle | Yining Han Jiaxing Wei Wei Feng Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healing Materials Today Bio Glucose oxidase Radicals Cerium ion Copper ion Wound healing |
| title | Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healing |
| title_full | Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healing |
| title_fullStr | Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healing |
| title_full_unstemmed | Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healing |
| title_short | Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healing |
| title_sort | hybrid of glucose oxidase and enzyme like cu ce efficient glucose scavenging and generation of hydroxyl hydroperoxyl and superoxide radicals for wound healing |
| topic | Glucose oxidase Radicals Cerium ion Copper ion Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S259000642500448X |
| work_keys_str_mv | AT yininghan hybridofglucoseoxidaseandenzymelikecuceefficientglucosescavengingandgenerationofhydroxylhydroperoxylandsuperoxideradicalsforwoundhealing AT jiaxingwei hybridofglucoseoxidaseandenzymelikecuceefficientglucosescavengingandgenerationofhydroxylhydroperoxylandsuperoxideradicalsforwoundhealing AT weifeng hybridofglucoseoxidaseandenzymelikecuceefficientglucosescavengingandgenerationofhydroxylhydroperoxylandsuperoxideradicalsforwoundhealing |