Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism
Abstract Osteosarcoma (OS) is the most common primary malignant bone tumor. Recent evidence suggests that the novel long noncoding RNA DHRS4 antisense RNA 1 (DHRS4-AS1) serves an important role in cancer progression and metastasis. However, its function and molecular mechanism in OS remain largely u...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-87246-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585852604121088 |
|---|---|
| author | Zhouzhou Tang Zhihao Li Guofeng Wu Jianjun Li Jianye Tan Lixin Zhu |
| author_facet | Zhouzhou Tang Zhihao Li Guofeng Wu Jianjun Li Jianye Tan Lixin Zhu |
| author_sort | Zhouzhou Tang |
| collection | DOAJ |
| description | Abstract Osteosarcoma (OS) is the most common primary malignant bone tumor. Recent evidence suggests that the novel long noncoding RNA DHRS4 antisense RNA 1 (DHRS4-AS1) serves an important role in cancer progression and metastasis. However, its function and molecular mechanism in OS remain largely unknown. In the present study, DHRS4-AS1 expression was detected in OS cells by quantitative PCR. Gain- and loss-of-function experiments were conducted to study the effects of DHRS4-AS1 on the proliferation and apoptosis of OS cells. The potential mechanism of DHRS4-AS1 was examined through bioinformatics analysis and rescue experiments. DHRS4-AS1 was downregulated in OS cell lines. DHRS4-AS1 depletion promoted proliferation and inhibited apoptosis in OS cells, whereas DHRS4-AS1 overexpression had the opposite effects. Further research suggested that DHRS4-AS1 inhibited OS progression by regulating the microRNA-362-5p/aminopeptidase puromycin sensitive axis. The present findings suggested that DHRS4-AS1 may serve as a potential therapeutic target for OS. |
| format | Article |
| id | doaj-art-7f63b8293e3f45b797f62180a71245be |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7f63b8293e3f45b797f62180a71245be2025-01-26T12:25:49ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-025-87246-7Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanismZhouzhou Tang0Zhihao Li1Guofeng Wu2Jianjun Li3Jianye Tan4Lixin Zhu5Department of Spinal Surgery, Zhujiang Hospital, Southern Medical UniversityDepartment of Spinal Surgery, Jingzhou Hospital Affiliated to Yangtze UniversityDepartment of Orthopedics, Southern University of Science and Technology HospitalDepartment of Spinal Surgery, Zhujiang Hospital, Southern Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang UniversityDepartment of Spinal Surgery, Zhujiang Hospital, Southern Medical UniversityAbstract Osteosarcoma (OS) is the most common primary malignant bone tumor. Recent evidence suggests that the novel long noncoding RNA DHRS4 antisense RNA 1 (DHRS4-AS1) serves an important role in cancer progression and metastasis. However, its function and molecular mechanism in OS remain largely unknown. In the present study, DHRS4-AS1 expression was detected in OS cells by quantitative PCR. Gain- and loss-of-function experiments were conducted to study the effects of DHRS4-AS1 on the proliferation and apoptosis of OS cells. The potential mechanism of DHRS4-AS1 was examined through bioinformatics analysis and rescue experiments. DHRS4-AS1 was downregulated in OS cell lines. DHRS4-AS1 depletion promoted proliferation and inhibited apoptosis in OS cells, whereas DHRS4-AS1 overexpression had the opposite effects. Further research suggested that DHRS4-AS1 inhibited OS progression by regulating the microRNA-362-5p/aminopeptidase puromycin sensitive axis. The present findings suggested that DHRS4-AS1 may serve as a potential therapeutic target for OS.https://doi.org/10.1038/s41598-025-87246-7OsteosarcomaDHRS4-AS1Long noncoding RNAsMiR-362-5pNPEPPS |
| spellingShingle | Zhouzhou Tang Zhihao Li Guofeng Wu Jianjun Li Jianye Tan Lixin Zhu Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism Scientific Reports Osteosarcoma DHRS4-AS1 Long noncoding RNAs MiR-362-5p NPEPPS |
| title | Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism |
| title_full | Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism |
| title_fullStr | Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism |
| title_full_unstemmed | Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism |
| title_short | Long noncoding RNA DHRS4 antisense RNA 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous RNA mechanism |
| title_sort | long noncoding rna dhrs4 antisense rna 1 suppresses osteosarcoma cell proliferation and promotes apoptosis through a competitive endogenous rna mechanism |
| topic | Osteosarcoma DHRS4-AS1 Long noncoding RNAs MiR-362-5p NPEPPS |
| url | https://doi.org/10.1038/s41598-025-87246-7 |
| work_keys_str_mv | AT zhouzhoutang longnoncodingrnadhrs4antisenserna1suppressesosteosarcomacellproliferationandpromotesapoptosisthroughacompetitiveendogenousrnamechanism AT zhihaoli longnoncodingrnadhrs4antisenserna1suppressesosteosarcomacellproliferationandpromotesapoptosisthroughacompetitiveendogenousrnamechanism AT guofengwu longnoncodingrnadhrs4antisenserna1suppressesosteosarcomacellproliferationandpromotesapoptosisthroughacompetitiveendogenousrnamechanism AT jianjunli longnoncodingrnadhrs4antisenserna1suppressesosteosarcomacellproliferationandpromotesapoptosisthroughacompetitiveendogenousrnamechanism AT jianyetan longnoncodingrnadhrs4antisenserna1suppressesosteosarcomacellproliferationandpromotesapoptosisthroughacompetitiveendogenousrnamechanism AT lixinzhu longnoncodingrnadhrs4antisenserna1suppressesosteosarcomacellproliferationandpromotesapoptosisthroughacompetitiveendogenousrnamechanism |