Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii
Natural polysaccharides named PEP-0.1-1, PEP-0-1 and PEP-0-2 from edible mushroom species Pleurotus eryngii were obtained in the present study. Results showed that molecular weights of these polysaccharides were 3235, 2041 and 23933 Da, respectively. Further, structural characterization revealed tha...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-09-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250245 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850080049922834432 |
|---|---|
| author | Han Wang Sai Ma Alfred Mugambi Mariga Qiuhui Hu Qian Xu Anxiang Su Ning Ma Gaoxing Ma |
| author_facet | Han Wang Sai Ma Alfred Mugambi Mariga Qiuhui Hu Qian Xu Anxiang Su Ning Ma Gaoxing Ma |
| author_sort | Han Wang |
| collection | DOAJ |
| description | Natural polysaccharides named PEP-0.1-1, PEP-0-1 and PEP-0-2 from edible mushroom species Pleurotus eryngii were obtained in the present study. Results showed that molecular weights of these polysaccharides were 3235, 2041 and 23933 Da, respectively. Further, structural characterization revealed that PEP-0.1-1 had a →4-α-D-Glcp-1→ backbone and contained →4)-α-D-Glcp and →4)-β-D-Glcp reducing end groups. PEP-0-1 backbone contained→4-α-D-Glcp-1→ and →6-α-3-O-Me-D-Galp-1→, and the side chains contained α-D-Glcp, β-D-Manp-1→ and α-D-Glcp-3→. However, PEP-0-2 backbone consisted of →4-α-DGlcp-1→ and →6-α-3-O-Me-D-Galp-(1→6)-α-D-Galp-1→ while the side chains contained α-D-Glcp and β-D-Manp-1→. Biological activity analysis was then carried out and found that all these polysaccharides could significantly suppress the relative mRNA expression of toll-like receptor 4, nitric oxide (NO), tumor necrosis factor-α, interleukin (IL)-1β and IL-6 in lipopolysaccharide (LPS)-induced inflammation of RAW264.7 cells, as well as the over secretion of the above cell cytokines. Moreover, Western blotting analysis revealed that all these purified fractions displayed significant inhibition effects on the expression of c-Jun N-terminal kinases protein induced by LPS in mitogen activated protein kinase pathway, along with the relieving on the inhibition effect of LPS on IκB-α protein expression. In summary, the information generated by the present study could provide a theoretical basis for the exploration of novel healthy food materials from edible mushroom with antiinflammation activities. |
| format | Article |
| id | doaj-art-7f586ea729f349e1a9e59b3a17c9691e |
| institution | DOAJ |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-7f586ea729f349e1a9e59b3a17c9691e2025-08-20T02:45:03ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-09-011353031304210.26599/FSHW.2022.9250245Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngiiHan Wang0Sai Ma1Alfred Mugambi Mariga2Qiuhui Hu3Qian Xu4Anxiang Su5Ning Ma6Gaoxing Ma7College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaSchool of Agriculture and Food Science, Meru University of Science and Technology, Meru 972-60200, KenyaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaNatural polysaccharides named PEP-0.1-1, PEP-0-1 and PEP-0-2 from edible mushroom species Pleurotus eryngii were obtained in the present study. Results showed that molecular weights of these polysaccharides were 3235, 2041 and 23933 Da, respectively. Further, structural characterization revealed that PEP-0.1-1 had a →4-α-D-Glcp-1→ backbone and contained →4)-α-D-Glcp and →4)-β-D-Glcp reducing end groups. PEP-0-1 backbone contained→4-α-D-Glcp-1→ and →6-α-3-O-Me-D-Galp-1→, and the side chains contained α-D-Glcp, β-D-Manp-1→ and α-D-Glcp-3→. However, PEP-0-2 backbone consisted of →4-α-DGlcp-1→ and →6-α-3-O-Me-D-Galp-(1→6)-α-D-Galp-1→ while the side chains contained α-D-Glcp and β-D-Manp-1→. Biological activity analysis was then carried out and found that all these polysaccharides could significantly suppress the relative mRNA expression of toll-like receptor 4, nitric oxide (NO), tumor necrosis factor-α, interleukin (IL)-1β and IL-6 in lipopolysaccharide (LPS)-induced inflammation of RAW264.7 cells, as well as the over secretion of the above cell cytokines. Moreover, Western blotting analysis revealed that all these purified fractions displayed significant inhibition effects on the expression of c-Jun N-terminal kinases protein induced by LPS in mitogen activated protein kinase pathway, along with the relieving on the inhibition effect of LPS on IκB-α protein expression. In summary, the information generated by the present study could provide a theoretical basis for the exploration of novel healthy food materials from edible mushroom with antiinflammation activities.https://www.sciopen.com/article/10.26599/FSHW.2022.9250245pleurotus eryngiipolysaccharidesstructural characterizationanti-inflammatory activity |
| spellingShingle | Han Wang Sai Ma Alfred Mugambi Mariga Qiuhui Hu Qian Xu Anxiang Su Ning Ma Gaoxing Ma Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii Food Science and Human Wellness pleurotus eryngii polysaccharides structural characterization anti-inflammatory activity |
| title | Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii |
| title_full | Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii |
| title_fullStr | Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii |
| title_full_unstemmed | Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii |
| title_short | Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii |
| title_sort | structural characterization and anti inflammatory activities of novel polysaccharides obtained from pleurotus eryngii |
| topic | pleurotus eryngii polysaccharides structural characterization anti-inflammatory activity |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250245 |
| work_keys_str_mv | AT hanwang structuralcharacterizationandantiinflammatoryactivitiesofnovelpolysaccharidesobtainedfrompleurotuseryngii AT saima structuralcharacterizationandantiinflammatoryactivitiesofnovelpolysaccharidesobtainedfrompleurotuseryngii AT alfredmugambimariga structuralcharacterizationandantiinflammatoryactivitiesofnovelpolysaccharidesobtainedfrompleurotuseryngii AT qiuhuihu structuralcharacterizationandantiinflammatoryactivitiesofnovelpolysaccharidesobtainedfrompleurotuseryngii AT qianxu structuralcharacterizationandantiinflammatoryactivitiesofnovelpolysaccharidesobtainedfrompleurotuseryngii AT anxiangsu structuralcharacterizationandantiinflammatoryactivitiesofnovelpolysaccharidesobtainedfrompleurotuseryngii AT ningma structuralcharacterizationandantiinflammatoryactivitiesofnovelpolysaccharidesobtainedfrompleurotuseryngii AT gaoxingma structuralcharacterizationandantiinflammatoryactivitiesofnovelpolysaccharidesobtainedfrompleurotuseryngii |