Screening of Lipophilic Fractions of Botanical Forms of Celery by GC/MS Method
Introduction. The search for new tools for the treatment and prevention of obesity – a socially significant disease characterized by high prevalence and the presence of a number of complications – is an urgent area of research. Literature data indicate the presence of pharmacological activity in dif...
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LLC Center of Pharmaceutical Analytics (LLC «CPHA»)
2022-09-01
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| Series: | Разработка и регистрация лекарственных средств |
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| Online Access: | https://www.pharmjournal.ru/jour/article/view/1300 |
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| author | E. S. Surbeeva N. Y. Sipkina S. I. Komova U. A. Efremova I. I. Terninko |
| author_facet | E. S. Surbeeva N. Y. Sipkina S. I. Komova U. A. Efremova I. I. Terninko |
| author_sort | E. S. Surbeeva |
| collection | DOAJ |
| description | Introduction. The search for new tools for the treatment and prevention of obesity – a socially significant disease characterized by high prevalence and the presence of a number of complications – is an urgent area of research. Literature data indicate the presence of pharmacological activity in different groups of BAC of plant origin – flavonoids, phenolic acids, phytosterols, etc. in relation to obesity and its associated diseases. In view of the significant accumulation of target groups of compounds, as well as the availability of a large and renewable raw material base, celery was chosen for research as a potential raw source of BAC contributing to the regulation of metabolic disorders.Aim. To investigate the component composition of lipophilic fractions of botanical forms of celery by GC/MS and to predict the pharmacological activity of identified compounds within the target group of pathologies using the web resource PASS Оnline.Materials and methods. Lipophilic fractions were prepared by chloroform extraction of dry crushed raw material of celery root, stalks and leaves in a Soxhlet apparatus. Obtained extracts were analyzed on gas chromatography-mass spectrometer "Clarus 600T" (PerkinElmer, USA) on the basis of the Collaborative Centre "Analytical Center" equipment. Interpretation of the mass spectra of the peaks on the chromatograms was carried out using the NIST-14 library, for further prediction of biological activity of the identified compounds we used web-resource PASS online.Results and discussion. The composition of lipophilic fractions of celery botanical forms is represented by terpenes, benzofurans, coumarins, fatty acids and their derivatives, phytosterols. A total of 50 compounds were found in extracts from celery root, of which 68 % were identified, stalks – 36 and 61 %; leaves – 22 and 64 %, respectively. Prediction of the pharmacological activity of individual compounds of celery showed the possibility of their use for the regulation of lipid and carbohydrate metabolism, cardiovascular disorders and liver diseases, which confirms the prospects of this study.Conclusion. The results have shown that celery roots and stalks are a source of terpenes, coumarins and benzofuran derivatives, in addition, the above-ground part of the plant shows high accumulation of phytosterols, which may have metabolic regulation activity and be used in the complex therapy and prevention of obesity. |
| format | Article |
| id | doaj-art-7f5094298c48496da98f0dff4eafc139 |
| institution | DOAJ |
| issn | 2305-2066 2658-5049 |
| language | Russian |
| publishDate | 2022-09-01 |
| publisher | LLC Center of Pharmaceutical Analytics (LLC «CPHA») |
| record_format | Article |
| series | Разработка и регистрация лекарственных средств |
| spelling | doaj-art-7f5094298c48496da98f0dff4eafc1392025-08-20T03:19:29ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492022-09-0111318119410.33380/2305-2066-2022-11-3-181-194999Screening of Lipophilic Fractions of Botanical Forms of Celery by GC/MS MethodE. S. Surbeeva0N. Y. Sipkina1S. I. Komova2U. A. Efremova3I. I. Terninko4St. Petersburg State Chemical and Pharmaceutical UniversitySt. Petersburg State Chemical and Pharmaceutical UniversitySt. Petersburg State Chemical and Pharmaceutical UniversitySt. Petersburg State Chemical and Pharmaceutical UniversitySt. Petersburg State Chemical and Pharmaceutical UniversityIntroduction. The search for new tools for the treatment and prevention of obesity – a socially significant disease characterized by high prevalence and the presence of a number of complications – is an urgent area of research. Literature data indicate the presence of pharmacological activity in different groups of BAC of plant origin – flavonoids, phenolic acids, phytosterols, etc. in relation to obesity and its associated diseases. In view of the significant accumulation of target groups of compounds, as well as the availability of a large and renewable raw material base, celery was chosen for research as a potential raw source of BAC contributing to the regulation of metabolic disorders.Aim. To investigate the component composition of lipophilic fractions of botanical forms of celery by GC/MS and to predict the pharmacological activity of identified compounds within the target group of pathologies using the web resource PASS Оnline.Materials and methods. Lipophilic fractions were prepared by chloroform extraction of dry crushed raw material of celery root, stalks and leaves in a Soxhlet apparatus. Obtained extracts were analyzed on gas chromatography-mass spectrometer "Clarus 600T" (PerkinElmer, USA) on the basis of the Collaborative Centre "Analytical Center" equipment. Interpretation of the mass spectra of the peaks on the chromatograms was carried out using the NIST-14 library, for further prediction of biological activity of the identified compounds we used web-resource PASS online.Results and discussion. The composition of lipophilic fractions of celery botanical forms is represented by terpenes, benzofurans, coumarins, fatty acids and their derivatives, phytosterols. A total of 50 compounds were found in extracts from celery root, of which 68 % were identified, stalks – 36 and 61 %; leaves – 22 and 64 %, respectively. Prediction of the pharmacological activity of individual compounds of celery showed the possibility of their use for the regulation of lipid and carbohydrate metabolism, cardiovascular disorders and liver diseases, which confirms the prospects of this study.Conclusion. The results have shown that celery roots and stalks are a source of terpenes, coumarins and benzofuran derivatives, in addition, the above-ground part of the plant shows high accumulation of phytosterols, which may have metabolic regulation activity and be used in the complex therapy and prevention of obesity.https://www.pharmjournal.ru/jour/article/view/1300celerybotanical formslipophilic fractiongc/mspass onlinecorrection of metabolic disorders |
| spellingShingle | E. S. Surbeeva N. Y. Sipkina S. I. Komova U. A. Efremova I. I. Terninko Screening of Lipophilic Fractions of Botanical Forms of Celery by GC/MS Method Разработка и регистрация лекарственных средств celery botanical forms lipophilic fraction gc/ms pass online correction of metabolic disorders |
| title | Screening of Lipophilic Fractions of Botanical Forms of Celery by GC/MS Method |
| title_full | Screening of Lipophilic Fractions of Botanical Forms of Celery by GC/MS Method |
| title_fullStr | Screening of Lipophilic Fractions of Botanical Forms of Celery by GC/MS Method |
| title_full_unstemmed | Screening of Lipophilic Fractions of Botanical Forms of Celery by GC/MS Method |
| title_short | Screening of Lipophilic Fractions of Botanical Forms of Celery by GC/MS Method |
| title_sort | screening of lipophilic fractions of botanical forms of celery by gc ms method |
| topic | celery botanical forms lipophilic fraction gc/ms pass online correction of metabolic disorders |
| url | https://www.pharmjournal.ru/jour/article/view/1300 |
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