Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteria
IntroductionStabilization of heavy metals through phosphate-solubilizing bacteria (PSB) induced phosphate precipitation and urease-producing bacteria (UPB) induced carbonate precipitation are promising bioremediation methods. However, little attention has been conducted on the combined action of the...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1525316/full |
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| author | Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Zhenghao Yan Yongfang Yan Shuangquan Li Shuangquan Li Shuangquan Li Shuangquan Li Shuangquan Li Mingjiao Yu Mingjiao Yu Mingjiao Yu Mingjiao Yu Mingjiao Yu Xiao Yan Xiao Yan Xiao Yan Xiao Yan Xiao Yan Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang |
| author_facet | Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Zhenghao Yan Yongfang Yan Shuangquan Li Shuangquan Li Shuangquan Li Shuangquan Li Shuangquan Li Mingjiao Yu Mingjiao Yu Mingjiao Yu Mingjiao Yu Mingjiao Yu Xiao Yan Xiao Yan Xiao Yan Xiao Yan Xiao Yan Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang |
| author_sort | Xuezhe Zhu |
| collection | DOAJ |
| description | IntroductionStabilization of heavy metals through phosphate-solubilizing bacteria (PSB) induced phosphate precipitation and urease-producing bacteria (UPB) induced carbonate precipitation are promising bioremediation methods. However, little attention has been conducted on the combined action of the above two bioremediations to stabilize heavy metals.MethodsPSB and UPB were isolated from the environment and their growth characteristics and antagonistic properties were studied. A simulated solution of acidic leachate was prepared based on heavy metal contaminated soil. Microbial consortium of PSB and UPB were constructed for the stabilization of heavy metals by optimizing carbon and nitrogen sources. The microstructural and compositional changes during the biostabilization process were more deeply analyzed using XRD, FT-IR and SEM-EDS.Results and discussionThe precipitation of heavy metals could be promoted effectively when soluble starch (10.2 g/L) was used as carbon source and urea (7.8 g/L) as nitrogen source. The stabilization rates for Cu, Zn, Cd, and Pb were 98.35, 99.78, 99.09, and 92.26%, respectively. The stabilization rates of the combined action of PSB and UPB were significantly higher than that of the two microorganisms alone. An in-depth analysis showed that the composite metals were precipitated as dense precipitate encased in carbonate and phosphate, and additionally could be stabilized in the form of biosorption. Finally, the stabilization mechanism of heavy metals based on biomineralization and biosorption is proposed. These findings provide new theoretical support for sustainable remediation and management strategies for composite heavy metal polluted areas. |
| format | Article |
| id | doaj-art-7f504cef0e964fd58918f6611d66bfdd |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-7f504cef0e964fd58918f6611d66bfdd2025-02-03T06:33:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15253161525316Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteriaXuezhe Zhu0Xuezhe Zhu1Xuezhe Zhu2Xuezhe Zhu3Xuezhe Zhu4Xuezhe Zhu5Yupin Zhou6Yupin Zhou7Yupin Zhou8Yupin Zhou9Yupin Zhou10Yupin Zhou11Zhenghao Yan12Yongfang Yan13Shuangquan Li14Shuangquan Li15Shuangquan Li16Shuangquan Li17Shuangquan Li18Mingjiao Yu19Mingjiao Yu20Mingjiao Yu21Mingjiao Yu22Mingjiao Yu23Xiao Yan24Xiao Yan25Xiao Yan26Xiao Yan27Xiao Yan28Mingjiang Zhang29Mingjiang Zhang30Mingjiang Zhang31Mingjiang Zhang32Mingjiang Zhang33National Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, ChinaSchool of Metallurgy, Northeastern University, Shenyang, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaGeneral Research Institute for Nonferrous Metals, Beijing, ChinaBeijing Engineering Research Center of Strategic Nonferrous Metals Green Manufacturing Technology, Beijing, ChinaGRIMAT Engineering Institute Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaGeneral Research Institute for Nonferrous Metals, Beijing, ChinaBeijing Engineering Research Center of Strategic Nonferrous Metals Green Manufacturing Technology, Beijing, ChinaGRIMAT Engineering Institute Co., Ltd., Beijing, ChinaGuobiao (Beijing) Testing & Certification Co., Ltd., China GRINM Group Co., Ltd., Beijing, ChinaSchool of Materials Science and Engineering, Henan University of Science and Technology, Luoyang, ChinaShougang Group Talent Development Institute, Beijing, ChinaNational Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaGeneral Research Institute for Nonferrous Metals, Beijing, ChinaBeijing Engineering Research Center of Strategic Nonferrous Metals Green Manufacturing Technology, Beijing, ChinaGRIMAT Engineering Institute Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaGeneral Research Institute for Nonferrous Metals, Beijing, ChinaBeijing Engineering Research Center of Strategic Nonferrous Metals Green Manufacturing Technology, Beijing, ChinaGRIMAT Engineering Institute Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaGeneral Research Institute for Nonferrous Metals, Beijing, ChinaBeijing Engineering Research Center of Strategic Nonferrous Metals Green Manufacturing Technology, Beijing, ChinaGRIMAT Engineering Institute Co., Ltd., Beijing, ChinaNational Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, ChinaGRINM Resources and Environment Tech. Co., Ltd., Beijing, ChinaGeneral Research Institute for Nonferrous Metals, Beijing, ChinaBeijing Engineering Research Center of Strategic Nonferrous Metals Green Manufacturing Technology, Beijing, ChinaGRIMAT Engineering Institute Co., Ltd., Beijing, ChinaIntroductionStabilization of heavy metals through phosphate-solubilizing bacteria (PSB) induced phosphate precipitation and urease-producing bacteria (UPB) induced carbonate precipitation are promising bioremediation methods. However, little attention has been conducted on the combined action of the above two bioremediations to stabilize heavy metals.MethodsPSB and UPB were isolated from the environment and their growth characteristics and antagonistic properties were studied. A simulated solution of acidic leachate was prepared based on heavy metal contaminated soil. Microbial consortium of PSB and UPB were constructed for the stabilization of heavy metals by optimizing carbon and nitrogen sources. The microstructural and compositional changes during the biostabilization process were more deeply analyzed using XRD, FT-IR and SEM-EDS.Results and discussionThe precipitation of heavy metals could be promoted effectively when soluble starch (10.2 g/L) was used as carbon source and urea (7.8 g/L) as nitrogen source. The stabilization rates for Cu, Zn, Cd, and Pb were 98.35, 99.78, 99.09, and 92.26%, respectively. The stabilization rates of the combined action of PSB and UPB were significantly higher than that of the two microorganisms alone. An in-depth analysis showed that the composite metals were precipitated as dense precipitate encased in carbonate and phosphate, and additionally could be stabilized in the form of biosorption. Finally, the stabilization mechanism of heavy metals based on biomineralization and biosorption is proposed. These findings provide new theoretical support for sustainable remediation and management strategies for composite heavy metal polluted areas.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1525316/fullheavy metal contaminationphosphate-solubilizing bacteria (PSB)urease-producing bacteria (UPB)biological stabilizationbiomineralization |
| spellingShingle | Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Xuezhe Zhu Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Yupin Zhou Zhenghao Yan Yongfang Yan Shuangquan Li Shuangquan Li Shuangquan Li Shuangquan Li Shuangquan Li Mingjiao Yu Mingjiao Yu Mingjiao Yu Mingjiao Yu Mingjiao Yu Xiao Yan Xiao Yan Xiao Yan Xiao Yan Xiao Yan Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang Mingjiang Zhang Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteria Frontiers in Microbiology heavy metal contamination phosphate-solubilizing bacteria (PSB) urease-producing bacteria (UPB) biological stabilization biomineralization |
| title | Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteria |
| title_full | Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteria |
| title_fullStr | Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteria |
| title_full_unstemmed | Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteria |
| title_short | Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteria |
| title_sort | stabilization effect and mechanism of heavy metals by microbial consortium of phosphate solubilizing bacteria and urease producing bacteria |
| topic | heavy metal contamination phosphate-solubilizing bacteria (PSB) urease-producing bacteria (UPB) biological stabilization biomineralization |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1525316/full |
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