HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway
Intraperitoneal adhesion is a common complication after abdominal surgery, which seriously affects the quality of life of patients. HuoXueTongFu Formula (HXTF) plays an important role in the prevention and treatment of intraperitoneal adhesions. However, the molecular-related mechanisms are still no...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/1769374 |
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| author | Min Zhao Yao-Yao Bian Li-Li Yang Yan-Qi Chen Ya-Jie Wang Yan-Ting Ma Yu-Qiong Pei Wen-Lin Li Li Zeng |
| author_facet | Min Zhao Yao-Yao Bian Li-Li Yang Yan-Qi Chen Ya-Jie Wang Yan-Ting Ma Yu-Qiong Pei Wen-Lin Li Li Zeng |
| author_sort | Min Zhao |
| collection | DOAJ |
| description | Intraperitoneal adhesion is a common complication after abdominal surgery, which seriously affects the quality of life of patients. HuoXueTongFu Formula (HXTF) plays an important role in the prevention and treatment of intraperitoneal adhesions. However, the molecular-related mechanisms are still not fully known. In this study, the model of Intrapetitoneal adhesion was established by cecum abrasion and treated with HXTF for one week. RAW264.7 cells were given LPS, IFN-γ, IL-4, HXTF-medicated serum, and PPAR-γ agonist/antagonist, respectively. Histopathology, flow cytometry, ELISA, real-time PCR, and Western blotting were used to further detect the related protein, M1/M2 polarization tendency, and PPAR-γ nuclear translocation. The deposition of collagen fibres reduced in the local area of rats after the operation with HXTF treatment. Similar to IL-4, HXTF induced a tendency for macrophages to polarize toward M2 and promoted peroxisome proliferator-activated receptor-gamma (PPAR-γ) nuclear translocation. Furthermore, the use of HXTF and PPAR-γ agonists downregulated macrophage M1 polarization-related factors IL-1, IL-6, and TNF-alpha and upregulated M2 polarization-related factors IL-4, IL-10, and TGF-beta 1. Meanwhile, the use of HXTF and PPAR-γ agonists downregulated the SOCS3/JAK2/STAT1 pathway and activated the SOCS1/STAT6/PPAR-γ pathway. These results show that HXTF may reduce intraperitoneal adhesion by inducing macrophage M2 polarization and regulating the SOCS/JAK2/STAT/PPAR-γ pathway. |
| format | Article |
| id | doaj-art-7f4a426548684bf38304bd7c3c420f6e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7f4a426548684bf38304bd7c3c420f6e2025-02-03T06:44:17ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/17693741769374HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling PathwayMin Zhao0Yao-Yao Bian1Li-Li Yang2Yan-Qi Chen3Ya-Jie Wang4Yan-Ting Ma5Yu-Qiong Pei6Wen-Lin Li7Li Zeng8The First School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaSchool of Nursing, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaThe First School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaThe First School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaThe Second School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaThe Second School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaThe Second School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaThe First School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaIntraperitoneal adhesion is a common complication after abdominal surgery, which seriously affects the quality of life of patients. HuoXueTongFu Formula (HXTF) plays an important role in the prevention and treatment of intraperitoneal adhesions. However, the molecular-related mechanisms are still not fully known. In this study, the model of Intrapetitoneal adhesion was established by cecum abrasion and treated with HXTF for one week. RAW264.7 cells were given LPS, IFN-γ, IL-4, HXTF-medicated serum, and PPAR-γ agonist/antagonist, respectively. Histopathology, flow cytometry, ELISA, real-time PCR, and Western blotting were used to further detect the related protein, M1/M2 polarization tendency, and PPAR-γ nuclear translocation. The deposition of collagen fibres reduced in the local area of rats after the operation with HXTF treatment. Similar to IL-4, HXTF induced a tendency for macrophages to polarize toward M2 and promoted peroxisome proliferator-activated receptor-gamma (PPAR-γ) nuclear translocation. Furthermore, the use of HXTF and PPAR-γ agonists downregulated macrophage M1 polarization-related factors IL-1, IL-6, and TNF-alpha and upregulated M2 polarization-related factors IL-4, IL-10, and TGF-beta 1. Meanwhile, the use of HXTF and PPAR-γ agonists downregulated the SOCS3/JAK2/STAT1 pathway and activated the SOCS1/STAT6/PPAR-γ pathway. These results show that HXTF may reduce intraperitoneal adhesion by inducing macrophage M2 polarization and regulating the SOCS/JAK2/STAT/PPAR-γ pathway.http://dx.doi.org/10.1155/2019/1769374 |
| spellingShingle | Min Zhao Yao-Yao Bian Li-Li Yang Yan-Qi Chen Ya-Jie Wang Yan-Ting Ma Yu-Qiong Pei Wen-Lin Li Li Zeng HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway Mediators of Inflammation |
| title | HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway |
| title_full | HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway |
| title_fullStr | HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway |
| title_full_unstemmed | HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway |
| title_short | HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway |
| title_sort | huoxuetongfu formula alleviates intraperitoneal adhesion by regulating macrophage polarization and the socs jak2 stat ppar γ signalling pathway |
| url | http://dx.doi.org/10.1155/2019/1769374 |
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