Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in rats

This study aims to evaluate the effects of Momordica charantia on hepatorenal toxicity caused by potassium bromate (KBrO3) in rats. Wistar rats were divided into 4 groups as control, KBrO3, bitter melon (MC), and KBrO3+MC. Examining the antioxidant enzyme grade of the kidney tissues, it was found t...

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Main Authors: Fatma Karagözoğlu, Ahmet Uyar, Hurrem Turan Akkoyun, Serdar Altun, Mahire Bayramoğlu Akkoyun, Sevinç Aydin, Şule Melek, Aydın Şükrü Bengü, Suat Ekin, Ahmet Bakir
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Language:English
Published: Universidad del Zulia 2025-06-01
Series:Revista Científica
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Online Access:https://produccioncientificaluz.org/index.php/cientifica/article/view/43921
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author Fatma Karagözoğlu
Ahmet Uyar
Hurrem Turan Akkoyun
Serdar Altun
Mahire Bayramoğlu Akkoyun
Sevinç Aydin
Şule Melek
Aydın Şükrü Bengü
Suat Ekin
Ahmet Bakir
author_facet Fatma Karagözoğlu
Ahmet Uyar
Hurrem Turan Akkoyun
Serdar Altun
Mahire Bayramoğlu Akkoyun
Sevinç Aydin
Şule Melek
Aydın Şükrü Bengü
Suat Ekin
Ahmet Bakir
author_sort Fatma Karagözoğlu
collection DOAJ
description This study aims to evaluate the effects of Momordica charantia on hepatorenal toxicity caused by potassium bromate (KBrO3) in rats. Wistar rats were divided into 4 groups as control, KBrO3, bitter melon (MC), and KBrO3+MC. Examining the antioxidant enzyme grade of the kidney tissues, it was found that the enzyme activities of catalase (CAT) (P<0.05), glutathione peroxidase (GSH–Px) (P<0.01), and superoxide dismutase (SOD) (P<0.01) decreased in the KBrO3 group in comparison to the control. There was a significant decrease (P<0.001) in glutathione (GSH) levels and an increase (P<0.01) in malondialdehyde (MDA) level in the KBrO3 group in comparison to the control. Examining the antioxidant enzyme activities in liver tissue, it was determined that CAT, GSH–Px, and SOD enzymes reduced significantly (P<0.05, P<0.01, and P<0.001, respectively) in the KBrO3 group in comparison to the control, and the enzyme activity of decreased CAT, GSH–Px, and SOD enzymes significantly elevated (P<0.01) in the MC group. There was a reduction in GSH level in the KBrO3 group in comparison to the control (P<0.01), while an increase was recorded in the KBrO3+MC group (P<0.05). MDA level in liver tissue increased in KBrO3 group in comparison to the control (P<0.01) and MC decreased the MDA level. Histopathological analysis results indicate severe degenerative and necrotic lesions in hepatorenal histoarchitecture of KBrO3 rats in comparison to the control. However, application of MC+KBrO3 significantly reduced the induced hepatorenal injury with a concomitant increase in histopathological lesions. From the immunohistochemical aspect, MC revealed apoptosis concomitant with the suppression of necrosis in the KBrO3-treated rats as demonstrated by the caspase-3 activity.
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spelling doaj-art-7f47adf4bf46423a96dab25a3be16d312025-08-20T03:25:07ZengUniversidad del ZuliaRevista Científica0798-22592521-97152025-06-0135210.52973/rcfcv-e35660Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in ratsFatma Karagözoğlu0Ahmet Uyar1Hurrem Turan Akkoyun2Serdar Altun3Mahire Bayramoğlu Akkoyun4Sevinç Aydin5Şule Melek6Aydın Şükrü Bengü7Suat Ekin8Ahmet Bakir9Dokuz Eylül University, Faculty of Veterinary Science, Department of Animal Nutrition and Nutritional Diseases. İzmir, Türkiye.Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Pathology. Hatay, Türkiye.Siirt University, Faculty of Veterinary Science, Department of Physiology. Siirt, Türkiye. Atatürk University, Faculty of Veterinary Science, Department of Pathology. Erzurum, Türkiye. Siirt University, Faculty of Veterinary Science, Department of Biochemistry. Siirt, Türkiye. Munzur University, Çemişgezek Vocational School. Tunceli, Türkiye.Bingöl University, Faculty of Veterinary Science, Department of Surgery. Bingöl, Türkiye.Bingöl University, Vocational School of Health Services. Bingöl, Türkiye.YYU University, Faculty of Sciences, Department of Chemistry. Van, Türkiye.YYU University, Faculty of Sciences, Department of Chemistry. Van, Türkiye. This study aims to evaluate the effects of Momordica charantia on hepatorenal toxicity caused by potassium bromate (KBrO3) in rats. Wistar rats were divided into 4 groups as control, KBrO3, bitter melon (MC), and KBrO3+MC. Examining the antioxidant enzyme grade of the kidney tissues, it was found that the enzyme activities of catalase (CAT) (P<0.05), glutathione peroxidase (GSH–Px) (P<0.01), and superoxide dismutase (SOD) (P<0.01) decreased in the KBrO3 group in comparison to the control. There was a significant decrease (P<0.001) in glutathione (GSH) levels and an increase (P<0.01) in malondialdehyde (MDA) level in the KBrO3 group in comparison to the control. Examining the antioxidant enzyme activities in liver tissue, it was determined that CAT, GSH–Px, and SOD enzymes reduced significantly (P<0.05, P<0.01, and P<0.001, respectively) in the KBrO3 group in comparison to the control, and the enzyme activity of decreased CAT, GSH–Px, and SOD enzymes significantly elevated (P<0.01) in the MC group. There was a reduction in GSH level in the KBrO3 group in comparison to the control (P<0.01), while an increase was recorded in the KBrO3+MC group (P<0.05). MDA level in liver tissue increased in KBrO3 group in comparison to the control (P<0.01) and MC decreased the MDA level. Histopathological analysis results indicate severe degenerative and necrotic lesions in hepatorenal histoarchitecture of KBrO3 rats in comparison to the control. However, application of MC+KBrO3 significantly reduced the induced hepatorenal injury with a concomitant increase in histopathological lesions. From the immunohistochemical aspect, MC revealed apoptosis concomitant with the suppression of necrosis in the KBrO3-treated rats as demonstrated by the caspase-3 activity. https://produccioncientificaluz.org/index.php/cientifica/article/view/43921Potassium bromateMomordica charantiahepatotoxicitynephrotoxicityrat
spellingShingle Fatma Karagözoğlu
Ahmet Uyar
Hurrem Turan Akkoyun
Serdar Altun
Mahire Bayramoğlu Akkoyun
Sevinç Aydin
Şule Melek
Aydın Şükrü Bengü
Suat Ekin
Ahmet Bakir
Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in rats
Revista Científica
Potassium bromate
Momordica charantia
hepatotoxicity
nephrotoxicity
rat
title Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in rats
title_full Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in rats
title_fullStr Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in rats
title_full_unstemmed Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in rats
title_short Protective effect of Momordica charantia against Hepatorenal toxicity induced by Potassium bromate (KBrO3) in rats
title_sort protective effect of momordica charantia against hepatorenal toxicity induced by potassium bromate kbro3 in rats
topic Potassium bromate
Momordica charantia
hepatotoxicity
nephrotoxicity
rat
url https://produccioncientificaluz.org/index.php/cientifica/article/view/43921
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