Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal model
Abstract Background This study aimed to assess the histological and radiographic effects of sodium hexametaphosphate (SHMP) as a direct pulp capping (DPC) agent in immature permanent dog premolars. Methods A split-mouth design was employed with three healthy 4-month-old Mongrel dogs, each having 36...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12903-024-05297-0 |
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| author | Mostafa Kamel Mohamed Mohamed Abdelfattah Abdelrahman Abdel-Razik Hashem Abdel-Razik Ahmad Abdel Hamid Elheeny |
| author_facet | Mostafa Kamel Mohamed Mohamed Abdelfattah Abdelrahman Abdel-Razik Hashem Abdel-Razik Ahmad Abdel Hamid Elheeny |
| author_sort | Mostafa Kamel Mohamed |
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| description | Abstract Background This study aimed to assess the histological and radiographic effects of sodium hexametaphosphate (SHMP) as a direct pulp capping (DPC) agent in immature permanent dog premolars. Methods A split-mouth design was employed with three healthy 4-month-old Mongrel dogs, each having 36 premolars. The premolars were randomly assigned to either SHMP or MTA. The specimens were stained with hematoxylin and eosin (H&E) and Masson’s trichrome, and histologically examined three months after the animals were sacrificed. To assess root maturity, radiographic changes in root length (RL), root surface area (RSA), and apical foramen width (AFW) were measured at baseline and after 3 months. Quantitative data were analyzed using the paired-sample t-test, while the qualitative data based on Stanley's histological scoring system were tested using the Monte Carlo exact test. The level of significance was set at 5%. Results Histological findings showed no significant differences between the two groups, except for the average thickness of the predentin and odontoblastic layers, which was significantly higher in the SHMP specimens (P < 0.0001). The frequencies of fully calcified dentin bridges and regularly arranged dentinal tubules were significantly higher in the SHMP specimens (P < 0.05). Both materials showed comparable radiographic measurements (P > 0.05), except for the change in RL, which was significantly longer in the SHMP group (P < 0.05). Conclusions There were no significant differences between SHMP and MTA in some respects. Histological evaluation showed that SHMP provided better bioinductive and biocompatible properties compared to MTA. Radiographically, both materials showed comparable root maturogenesis outcomes, except for the significant increase in RL in the SHMP group. SHMP may be a suitable alternative material for DPC in the treatment of immature permanent teeth. |
| format | Article |
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| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-7f4590befaa848d980efa3ec307413ea2025-01-05T12:48:23ZengBMCBMC Oral Health1472-68312025-01-0125111310.1186/s12903-024-05297-0Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal modelMostafa Kamel Mohamed0Mohamed Abdelfattah Abdelrahman1Abdel-Razik Hashem Abdel-Razik2Ahmad Abdel Hamid Elheeny3Paediatric and Community Dentistry, Faculty of Dentistry, Minia UniversitySurgery, Anestheiology, and Radiology Department, Faculty of Veterinary Medicine, Minia UniversityFaculty of Veterinary Medicine, Beni-Suef UniversityPaediatric and Community Dentistry, Faculty of Dentistry, Minia UniversityAbstract Background This study aimed to assess the histological and radiographic effects of sodium hexametaphosphate (SHMP) as a direct pulp capping (DPC) agent in immature permanent dog premolars. Methods A split-mouth design was employed with three healthy 4-month-old Mongrel dogs, each having 36 premolars. The premolars were randomly assigned to either SHMP or MTA. The specimens were stained with hematoxylin and eosin (H&E) and Masson’s trichrome, and histologically examined three months after the animals were sacrificed. To assess root maturity, radiographic changes in root length (RL), root surface area (RSA), and apical foramen width (AFW) were measured at baseline and after 3 months. Quantitative data were analyzed using the paired-sample t-test, while the qualitative data based on Stanley's histological scoring system were tested using the Monte Carlo exact test. The level of significance was set at 5%. Results Histological findings showed no significant differences between the two groups, except for the average thickness of the predentin and odontoblastic layers, which was significantly higher in the SHMP specimens (P < 0.0001). The frequencies of fully calcified dentin bridges and regularly arranged dentinal tubules were significantly higher in the SHMP specimens (P < 0.05). Both materials showed comparable radiographic measurements (P > 0.05), except for the change in RL, which was significantly longer in the SHMP group (P < 0.05). Conclusions There were no significant differences between SHMP and MTA in some respects. Histological evaluation showed that SHMP provided better bioinductive and biocompatible properties compared to MTA. Radiographically, both materials showed comparable root maturogenesis outcomes, except for the significant increase in RL in the SHMP group. SHMP may be a suitable alternative material for DPC in the treatment of immature permanent teeth.https://doi.org/10.1186/s12903-024-05297-0Mineral trioxide aggregatePulp cappingRegenerative endodontics |
| spellingShingle | Mostafa Kamel Mohamed Mohamed Abdelfattah Abdelrahman Abdel-Razik Hashem Abdel-Razik Ahmad Abdel Hamid Elheeny Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal model BMC Oral Health Mineral trioxide aggregate Pulp capping Regenerative endodontics |
| title | Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal model |
| title_full | Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal model |
| title_fullStr | Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal model |
| title_full_unstemmed | Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal model |
| title_short | Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal model |
| title_sort | histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate shmp as a novel direct pulp capping material in an animal model |
| topic | Mineral trioxide aggregate Pulp capping Regenerative endodontics |
| url | https://doi.org/10.1186/s12903-024-05297-0 |
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