Preliminary analysis of the diagnostic value and mechanism of action of TGFbI and S100A4 in hepatocellular carcinoma
Background: The aim was to analyse the diagnostic value of transforming growth factor-beta-induced protein (TGF I) and S100 calcium-binding protein A4 (S100A4) on hepatocellular carcinoma (HCC) and to explore further the effects of TGF I and S100A4 on ferroptosis in HCC cells. Methods: We retrospect...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Society of Medical Biochemists of Serbia, Belgrade
2025-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2025/1452-82582503400Z.pdf |
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| Summary: | Background: The aim was to analyse the diagnostic value of transforming growth factor-beta-induced protein (TGF I) and S100 calcium-binding protein A4 (S100A4) on hepatocellular carcinoma (HCC) and to explore further the effects of TGF I and S100A4 on ferroptosis in HCC cells. Methods: We retrospectively analysed 76 patients with HCC admitted to our hospital from October 2022 to June 2023 and detected the differences in the expression of TGF I and S100A4 in cancerous tissues and paracancerous tissues to analyse their diagnostic and prognostic assessment value for HCC. Additionally, the HCC cell line HepG2 was purchased and transfected with TGF I and S100A4 abnormal expression plasmids to check changes in cell viability, oxidative stress damage, mitochondrial damage, and ferroptosis. Results: TGF I and S100A4 were upregulated in HCC tissues (P<0.05), and their combined detection exhibited excellent diagnostic effects for HCC. The levels of TGF I and S100A4 in patients who died prognostically were higher than those in surviving patients (P<0.05). An increase in the levels of TGF I and S100A4 indicates an elevated risk of prognostic death in patients. Upregulating TGF I and S100A4 expression in cell experiments activated HepG2 activity, inhibited apoptosis, mitochondrial and oxidative stress damage, and improved cell ferroptosis. Conclusions: TGF I and S100A4 are elevated in HCC and can potentially be clinical diagnostic indicators of HCC. |
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| ISSN: | 1452-8258 1452-8266 |