Effects of dietary nucleotide and yeast extract supplementation on survival, growth, and post-metamorphic spinal deformities in Japanese eel Anguilla japonica from the leptocephalus to glass eel stage
This study assessed the effects of dietary nucleotide (NT) and yeast extract supplementation on the survival, growth, and post-metamorphic spinal deformities of Japanese eel (Anguilla japonica) from leptocephalus to glass eel stages. Four dietary levels of a purified NT mixture of disodium salts con...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003990 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849242852125573120 |
|---|---|
| author | Tadao Jinbo Masato Higuchi Takeshi Hano Hirofumi Furuita Kazuharu Nomura Takashi Yatabe Hiroshi Suzuki Miyuki Mekuchi Takashi Ishikawa Youhei Fukui Nobuto Kaneko Yukinori Kazeto |
| author_facet | Tadao Jinbo Masato Higuchi Takeshi Hano Hirofumi Furuita Kazuharu Nomura Takashi Yatabe Hiroshi Suzuki Miyuki Mekuchi Takashi Ishikawa Youhei Fukui Nobuto Kaneko Yukinori Kazeto |
| author_sort | Tadao Jinbo |
| collection | DOAJ |
| description | This study assessed the effects of dietary nucleotide (NT) and yeast extract supplementation on the survival, growth, and post-metamorphic spinal deformities of Japanese eel (Anguilla japonica) from leptocephalus to glass eel stages. Four dietary levels of a purified NT mixture of disodium salts containing equal amounts of inosine 5′-monophosphate (IMP), adenosine 5′-monophosphate (AMP), guanosine 5′-monophosphate (GMP), uridine 5′-monophosphate (UMP), and cytidine 5′-monophosphate (CMP) were added to the basal diet at increasing concentrations of 0.03 (D1), 0.49 (D2), 0.97 (D3), 1.92 (D4), and 3.76 % (D5) dry matter. Rearing trials were conducted from 6–80 days post-hatching (DPH) in experiment 1 and from 81–340 DPH in experiment 2. In experiment 3, D1 and D6 modified D1 by incorporating yeast extract with a high NT content at 3.39 % dry matter and used in the rearing trials from 81–321 DPH. In experiment 1, the survival rates of the NT-supplemented groups were significantly higher than that of the D1 group. Additionally, larvae in the D5 group at 20 and 60 DPH and larvae in the D3–5 groups at 40 DPH displayed significantly greater total lengths (TL) than those in the D1 group. The body depth (BD) to TL ratio in the D1 group was significantly higher than that in the other groups at 60 and 80 DPH. In experiment 2, there were no significant differences in growth; however, the survival rate demonstrated a similar trend to that of experiment 1 between the groups. Additionally, the proportion of severe spinal abnormalities in metamorphosed glass eels reduced with increasing NT content in the diets compared with that in the D1 group. Global metabolite analysis revealed that some nucleic acids and their relatives were present to a significantly greater degree in the muscle of leptocephali in the D5 group than those in the D1 group. In experiment 3, there were no significant differences in the survival rate, whereas the BD-to-TL ratio in the D1 group was significantly higher than that in the D6 group. Additionally, the proportion of severe spinal abnormalities in metamorphosed glass eels was significantly reduced in the D6 group compared to that in the D1 group. These results indicate that NT supplementation is essential for enhancing the health and quality of eel larvae and is a crucial strategy for sustainable aquaculture practices. |
| format | Article |
| id | doaj-art-7f3dff61e8b24594bb1454a84bdf7526 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-7f3dff61e8b24594bb1454a84bdf75262025-08-20T03:59:40ZengElsevierAquaculture Reports2352-51342025-09-014310301310.1016/j.aqrep.2025.103013Effects of dietary nucleotide and yeast extract supplementation on survival, growth, and post-metamorphic spinal deformities in Japanese eel Anguilla japonica from the leptocephalus to glass eel stageTadao Jinbo0Masato Higuchi1Takeshi Hano2Hirofumi Furuita3Kazuharu Nomura4Takashi Yatabe5Hiroshi Suzuki6Miyuki Mekuchi7Takashi Ishikawa8Youhei Fukui9Nobuto Kaneko10Yukinori Kazeto11Shibushi Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 205 Natsui, Shibushi, Kagoshima 899-7101, Japan; Corresponding author.Nagasaki Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 1551-8 Taira-machi, Nagasaki 851-2213, JapanHatsukaichi Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, JapanNansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minami-ise, Watarai, Mie 516-0193, JapanNansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minami-ise, Watarai, Mie 516-0193, JapanMinami-izu Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, Irozaki, 183-2 Minamiizu, Kamo, Shizuoka 415-0156, JapanShibushi Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 205 Natsui, Shibushi, Kagoshima 899-7101, JapanYokohama Field Station, Fisheries Resources Institute, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazaw, Yokohama, Kanagawa 236-8648, JapanNansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minami-ise, Watarai, Mie 516-0193, JapanNansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minami-ise, Watarai, Mie 516-0193, JapanNansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minami-ise, Watarai, Mie 516-0193, JapanMinami-izu Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, Irozaki, 183-2 Minamiizu, Kamo, Shizuoka 415-0156, JapanThis study assessed the effects of dietary nucleotide (NT) and yeast extract supplementation on the survival, growth, and post-metamorphic spinal deformities of Japanese eel (Anguilla japonica) from leptocephalus to glass eel stages. Four dietary levels of a purified NT mixture of disodium salts containing equal amounts of inosine 5′-monophosphate (IMP), adenosine 5′-monophosphate (AMP), guanosine 5′-monophosphate (GMP), uridine 5′-monophosphate (UMP), and cytidine 5′-monophosphate (CMP) were added to the basal diet at increasing concentrations of 0.03 (D1), 0.49 (D2), 0.97 (D3), 1.92 (D4), and 3.76 % (D5) dry matter. Rearing trials were conducted from 6–80 days post-hatching (DPH) in experiment 1 and from 81–340 DPH in experiment 2. In experiment 3, D1 and D6 modified D1 by incorporating yeast extract with a high NT content at 3.39 % dry matter and used in the rearing trials from 81–321 DPH. In experiment 1, the survival rates of the NT-supplemented groups were significantly higher than that of the D1 group. Additionally, larvae in the D5 group at 20 and 60 DPH and larvae in the D3–5 groups at 40 DPH displayed significantly greater total lengths (TL) than those in the D1 group. The body depth (BD) to TL ratio in the D1 group was significantly higher than that in the other groups at 60 and 80 DPH. In experiment 2, there were no significant differences in growth; however, the survival rate demonstrated a similar trend to that of experiment 1 between the groups. Additionally, the proportion of severe spinal abnormalities in metamorphosed glass eels reduced with increasing NT content in the diets compared with that in the D1 group. Global metabolite analysis revealed that some nucleic acids and their relatives were present to a significantly greater degree in the muscle of leptocephali in the D5 group than those in the D1 group. In experiment 3, there were no significant differences in the survival rate, whereas the BD-to-TL ratio in the D1 group was significantly higher than that in the D6 group. Additionally, the proportion of severe spinal abnormalities in metamorphosed glass eels was significantly reduced in the D6 group compared to that in the D1 group. These results indicate that NT supplementation is essential for enhancing the health and quality of eel larvae and is a crucial strategy for sustainable aquaculture practices.http://www.sciencedirect.com/science/article/pii/S2352513425003990Anguilla japonicaLeptocephaliNucleotideLarval dietSpinal deformity |
| spellingShingle | Tadao Jinbo Masato Higuchi Takeshi Hano Hirofumi Furuita Kazuharu Nomura Takashi Yatabe Hiroshi Suzuki Miyuki Mekuchi Takashi Ishikawa Youhei Fukui Nobuto Kaneko Yukinori Kazeto Effects of dietary nucleotide and yeast extract supplementation on survival, growth, and post-metamorphic spinal deformities in Japanese eel Anguilla japonica from the leptocephalus to glass eel stage Aquaculture Reports Anguilla japonica Leptocephali Nucleotide Larval diet Spinal deformity |
| title | Effects of dietary nucleotide and yeast extract supplementation on survival, growth, and post-metamorphic spinal deformities in Japanese eel Anguilla japonica from the leptocephalus to glass eel stage |
| title_full | Effects of dietary nucleotide and yeast extract supplementation on survival, growth, and post-metamorphic spinal deformities in Japanese eel Anguilla japonica from the leptocephalus to glass eel stage |
| title_fullStr | Effects of dietary nucleotide and yeast extract supplementation on survival, growth, and post-metamorphic spinal deformities in Japanese eel Anguilla japonica from the leptocephalus to glass eel stage |
| title_full_unstemmed | Effects of dietary nucleotide and yeast extract supplementation on survival, growth, and post-metamorphic spinal deformities in Japanese eel Anguilla japonica from the leptocephalus to glass eel stage |
| title_short | Effects of dietary nucleotide and yeast extract supplementation on survival, growth, and post-metamorphic spinal deformities in Japanese eel Anguilla japonica from the leptocephalus to glass eel stage |
| title_sort | effects of dietary nucleotide and yeast extract supplementation on survival growth and post metamorphic spinal deformities in japanese eel anguilla japonica from the leptocephalus to glass eel stage |
| topic | Anguilla japonica Leptocephali Nucleotide Larval diet Spinal deformity |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003990 |
| work_keys_str_mv | AT tadaojinbo effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT masatohiguchi effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT takeshihano effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT hirofumifuruita effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT kazuharunomura effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT takashiyatabe effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT hiroshisuzuki effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT miyukimekuchi effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT takashiishikawa effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT youheifukui effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT nobutokaneko effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage AT yukinorikazeto effectsofdietarynucleotideandyeastextractsupplementationonsurvivalgrowthandpostmetamorphicspinaldeformitiesinjapaneseeelanguillajaponicafromtheleptocephalustoglasseelstage |