Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro
Seed cells of articular cartilage tissue engineering face many obstacles in their application because of the dedifferentiation of chondrocytes or unstable chondrogenic differentiation status of pluripotent stem cells. To overcome mentioned dilemmas, a simulation of the articular cartilage microenvir...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5130152 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850215077188206592 |
|---|---|
| author | Yu Zhang Shuyun Liu Weimin Guo Chunxiang Hao Mingjie Wang Xu Li Xueliang Zhang Mingxue Chen Zehao Wang Xiang Sui Jiang Peng Yu Wang Shibi Lu Quanyi Guo |
| author_facet | Yu Zhang Shuyun Liu Weimin Guo Chunxiang Hao Mingjie Wang Xu Li Xueliang Zhang Mingxue Chen Zehao Wang Xiang Sui Jiang Peng Yu Wang Shibi Lu Quanyi Guo |
| author_sort | Yu Zhang |
| collection | DOAJ |
| description | Seed cells of articular cartilage tissue engineering face many obstacles in their application because of the dedifferentiation of chondrocytes or unstable chondrogenic differentiation status of pluripotent stem cells. To overcome mentioned dilemmas, a simulation of the articular cartilage microenvironment was constructed by primary articular cartilage cells (pACs) and acellular cartilage extracellular matrix- (ACECM-) oriented scaffold cocultured with human umbilical cord Wharton’s jelly-derived mesenchymal stem cells (hWJMSCs) in vitro. The coculture groups showed more affluent cartilage special matrix ingredients including collagen II and aggrecan based on the results of histological staining and western blotting and cut down as many pACs as possible. The RT-PCR and cell viability experiments also demonstrated that hWJMSCs were successfully induced to differentiate into chondrocytes when cultured in the simulated cartilage microenvironment, as confirmed by the significant upregulation of collagen II and aggrecan, while the cell proliferation activity of pACs was significantly improved by cell-cell interactions. Therefore, compared with monoculture and chondrogenic induction of inducers, coculture providing a simulated native articular microenvironment was a potential and temperate way to regulate the biological behaviors of pACs and hWJMSCs to regenerate the hyaline articular cartilage. |
| format | Article |
| id | doaj-art-7f3286cd320146eea414aa5fdcacefc7 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-7f3286cd320146eea414aa5fdcacefc72025-08-20T02:08:43ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/51301525130152Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In VitroYu Zhang0Shuyun Liu1Weimin Guo2Chunxiang Hao3Mingjie Wang4Xu Li5Xueliang Zhang6Mingxue Chen7Zehao Wang8Xiang Sui9Jiang Peng10Yu Wang11Shibi Lu12Quanyi Guo13Institute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Anesthesia, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaSchool of Medicine, Nankai University, Tianjin 300071, ChinaShanxi Traditional Chinese, No. 46 Binzhou West Street, Yingze District, Taiyuan 030001, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaInstitute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, ChinaSeed cells of articular cartilage tissue engineering face many obstacles in their application because of the dedifferentiation of chondrocytes or unstable chondrogenic differentiation status of pluripotent stem cells. To overcome mentioned dilemmas, a simulation of the articular cartilage microenvironment was constructed by primary articular cartilage cells (pACs) and acellular cartilage extracellular matrix- (ACECM-) oriented scaffold cocultured with human umbilical cord Wharton’s jelly-derived mesenchymal stem cells (hWJMSCs) in vitro. The coculture groups showed more affluent cartilage special matrix ingredients including collagen II and aggrecan based on the results of histological staining and western blotting and cut down as many pACs as possible. The RT-PCR and cell viability experiments also demonstrated that hWJMSCs were successfully induced to differentiate into chondrocytes when cultured in the simulated cartilage microenvironment, as confirmed by the significant upregulation of collagen II and aggrecan, while the cell proliferation activity of pACs was significantly improved by cell-cell interactions. Therefore, compared with monoculture and chondrogenic induction of inducers, coculture providing a simulated native articular microenvironment was a potential and temperate way to regulate the biological behaviors of pACs and hWJMSCs to regenerate the hyaline articular cartilage.http://dx.doi.org/10.1155/2019/5130152 |
| spellingShingle | Yu Zhang Shuyun Liu Weimin Guo Chunxiang Hao Mingjie Wang Xu Li Xueliang Zhang Mingxue Chen Zehao Wang Xiang Sui Jiang Peng Yu Wang Shibi Lu Quanyi Guo Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro Stem Cells International |
| title | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro |
| title_full | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro |
| title_fullStr | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro |
| title_full_unstemmed | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro |
| title_short | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro |
| title_sort | coculture of hwjmscs and pacs in oriented scaffold enhances hyaline cartilage regeneration in vitro |
| url | http://dx.doi.org/10.1155/2019/5130152 |
| work_keys_str_mv | AT yuzhang cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT shuyunliu cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT weiminguo cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT chunxianghao cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT mingjiewang cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT xuli cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT xueliangzhang cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT mingxuechen cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT zehaowang cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT xiangsui cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT jiangpeng cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT yuwang cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT shibilu cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro AT quanyiguo cocultureofhwjmscsandpacsinorientedscaffoldenhanceshyalinecartilageregenerationinvitro |