Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric Cancer
Exosomes (Exos) are emerging as noninvasive biomarkers for diagnosis and progression monitoring of gastric cancer (GC). However, the heterogeneity discrimination and ultrasensitive quantification of Exos presents a considerable analytical challenge, thereby impeding severely their clinical applicati...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2025-01-01
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| Series: | Research |
| Online Access: | https://spj.science.org/doi/10.34133/research.0625 |
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| author | Qingzhe Jia Zhaofu Ma Yujia Wang Mingjin Zhang Guijun Zou Bin Lan Songyan Li Zeqiu Lao Wenbin Shen Jing Lou Yanan Jiao Xiaohui Du |
| author_facet | Qingzhe Jia Zhaofu Ma Yujia Wang Mingjin Zhang Guijun Zou Bin Lan Songyan Li Zeqiu Lao Wenbin Shen Jing Lou Yanan Jiao Xiaohui Du |
| author_sort | Qingzhe Jia |
| collection | DOAJ |
| description | Exosomes (Exos) are emerging as noninvasive biomarkers for diagnosis and progression monitoring of gastric cancer (GC). However, the heterogeneity discrimination and ultrasensitive quantification of Exos presents a considerable analytical challenge, thereby impeding severely their clinical application. Herein, we propose an integrated terahertz metasensing platform for the discrimination of Exos in distinct subtypes of GC in a single step—through the simultaneous evaluation of the category and richness level of Exos membrane proteins. Characterized by dual-sided independent sensing capabilities with enhanced sensitivity (169 and 325 GHz per refractive index unit, respectively), the metasensor functionalized with antibodies simultaneously reflects the content of 2 membrane proteins in the terahertz spectral response. Our approach concurrently completes accurate differentiation and precise quantification of GC-subtype Exos by integrating dual-sided sensing information in merely a single assay. The dual-sided sensing design enhances the reliability of detection results. Moreover, combined with the signal amplification of gold nanoparticles, the platform experimentally demonstrates a superior dynamic response to Exos concentrations spanning from 1 × 104 to 1 × 108 particles/ml, with the limit of detection being 1 × 104 particles/ml. This work provides new insights into multisensing metasurface design and paves the way for precise and personalized cancer treatment through the specific sensing of Exos. |
| format | Article |
| id | doaj-art-7f30b30ece5546e8bbb1f0208e376098 |
| institution | DOAJ |
| issn | 2639-5274 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Research |
| spelling | doaj-art-7f30b30ece5546e8bbb1f0208e3760982025-08-20T03:12:20ZengAmerican Association for the Advancement of Science (AAAS)Research2639-52742025-01-01810.34133/research.0625Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric CancerQingzhe Jia0Zhaofu Ma1Yujia Wang2Mingjin Zhang3Guijun Zou4Bin Lan5Songyan Li6Zeqiu Lao7Wenbin Shen8Jing Lou9Yanan Jiao10Xiaohui Du11Department of General Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Department of General Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Department of Traditional Chinese Medicine, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Department of General Surgery, The 901st Hospital of PLA, Hefei 230031, Anhui Province, China.Department of General Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Department of General Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Department of General Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Department of General Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Department of Critical Care Medicine in 83rd Group Army Hospital, Xinxiang City 453000, Henan Province, China.Innovation Laboratory of Terahertz Biophysics, National Innovation Institute of Defense Technology, Beijing 100071, China.Department of General Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Department of General Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.Exosomes (Exos) are emerging as noninvasive biomarkers for diagnosis and progression monitoring of gastric cancer (GC). However, the heterogeneity discrimination and ultrasensitive quantification of Exos presents a considerable analytical challenge, thereby impeding severely their clinical application. Herein, we propose an integrated terahertz metasensing platform for the discrimination of Exos in distinct subtypes of GC in a single step—through the simultaneous evaluation of the category and richness level of Exos membrane proteins. Characterized by dual-sided independent sensing capabilities with enhanced sensitivity (169 and 325 GHz per refractive index unit, respectively), the metasensor functionalized with antibodies simultaneously reflects the content of 2 membrane proteins in the terahertz spectral response. Our approach concurrently completes accurate differentiation and precise quantification of GC-subtype Exos by integrating dual-sided sensing information in merely a single assay. The dual-sided sensing design enhances the reliability of detection results. Moreover, combined with the signal amplification of gold nanoparticles, the platform experimentally demonstrates a superior dynamic response to Exos concentrations spanning from 1 × 104 to 1 × 108 particles/ml, with the limit of detection being 1 × 104 particles/ml. This work provides new insights into multisensing metasurface design and paves the way for precise and personalized cancer treatment through the specific sensing of Exos.https://spj.science.org/doi/10.34133/research.0625 |
| spellingShingle | Qingzhe Jia Zhaofu Ma Yujia Wang Mingjin Zhang Guijun Zou Bin Lan Songyan Li Zeqiu Lao Wenbin Shen Jing Lou Yanan Jiao Xiaohui Du Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric Cancer Research |
| title | Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric Cancer |
| title_full | Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric Cancer |
| title_fullStr | Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric Cancer |
| title_full_unstemmed | Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric Cancer |
| title_short | Integrated Single-Step Terahertz Metasensing for Simultaneous Detection Based on Exosomal Membrane Proteins Enables Pathological Typing of Gastric Cancer |
| title_sort | integrated single step terahertz metasensing for simultaneous detection based on exosomal membrane proteins enables pathological typing of gastric cancer |
| url | https://spj.science.org/doi/10.34133/research.0625 |
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