Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster
Chemically investigating the marine-derived <i>Streptomyces parvus</i> 1268 led to the isolation of a new compound of carpatamide I (<b>1</b>). Subsequent genomic analysis identified its candidate biosynthetic gene cluster <i>ctd</i> of approximately 44 kb. In ord...
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| Format: | Article |
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MDPI AG
2024-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/22/11/521 |
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| author | Shu-Mei Shen Yun-Chang Xie Li-Rong Tu Miao-Er Wu Yan-Min Wang Chun-Hui Song Yu-Hui Sun Ming-He Luo |
| author_facet | Shu-Mei Shen Yun-Chang Xie Li-Rong Tu Miao-Er Wu Yan-Min Wang Chun-Hui Song Yu-Hui Sun Ming-He Luo |
| author_sort | Shu-Mei Shen |
| collection | DOAJ |
| description | Chemically investigating the marine-derived <i>Streptomyces parvus</i> 1268 led to the isolation of a new compound of carpatamide I (<b>1</b>). Subsequent genomic analysis identified its candidate biosynthetic gene cluster <i>ctd</i> of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J–M (<b>2</b>–<b>5</b>). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds <b>1</b>–<b>5</b> displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2. |
| format | Article |
| id | doaj-art-7f2f192af40246929489519e71bc988a |
| institution | OA Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-7f2f192af40246929489519e71bc988a2025-08-20T01:53:54ZengMDPI AGMarine Drugs1660-33972024-11-01221152110.3390/md22110521Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene ClusterShu-Mei Shen0Yun-Chang Xie1Li-Rong Tu2Miao-Er Wu3Yan-Min Wang4Chun-Hui Song5Yu-Hui Sun6Ming-He Luo7School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, ChinaCollege of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaSchool of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, ChinaCollege of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaCollege of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaCollege of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaSchool of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, ChinaSchool of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, ChinaChemically investigating the marine-derived <i>Streptomyces parvus</i> 1268 led to the isolation of a new compound of carpatamide I (<b>1</b>). Subsequent genomic analysis identified its candidate biosynthetic gene cluster <i>ctd</i> of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J–M (<b>2</b>–<b>5</b>). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds <b>1</b>–<b>5</b> displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2.https://www.mdpi.com/1660-3397/22/11/521carpatamidesmanumycinnatural productsgenome mining |
| spellingShingle | Shu-Mei Shen Yun-Chang Xie Li-Rong Tu Miao-Er Wu Yan-Min Wang Chun-Hui Song Yu-Hui Sun Ming-He Luo Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster Marine Drugs carpatamides manumycin natural products genome mining |
| title | Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster |
| title_full | Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster |
| title_fullStr | Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster |
| title_full_unstemmed | Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster |
| title_short | Genome-Based Mining of Carpatamides I–M and Their Candidate Biosynthetic Gene Cluster |
| title_sort | genome based mining of carpatamides i m and their candidate biosynthetic gene cluster |
| topic | carpatamides manumycin natural products genome mining |
| url | https://www.mdpi.com/1660-3397/22/11/521 |
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