Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells
The ability of mesenchymal stromal (stem) cells (MSCs) to be mobilised from their local depot towards sites of injury and to participate in tissue repair makes these cells promising candidates for cell therapy. Physiological O2 tension in an MSC niche in vivo is about 4–7%. However, most in vitro st...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7260562 |
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| author | Olga O. Udartseva Margarita V. Lobanova Elena R. Andreeva Sergey V. Buravkov Irina V. Ogneva Ludmila B. Buravkova |
| author_facet | Olga O. Udartseva Margarita V. Lobanova Elena R. Andreeva Sergey V. Buravkov Irina V. Ogneva Ludmila B. Buravkova |
| author_sort | Olga O. Udartseva |
| collection | DOAJ |
| description | The ability of mesenchymal stromal (stem) cells (MSCs) to be mobilised from their local depot towards sites of injury and to participate in tissue repair makes these cells promising candidates for cell therapy. Physiological O2 tension in an MSC niche in vivo is about 4–7%. However, most in vitro studies of MSC functional activity are performed at 20% O2. Therefore, this study focused on the effects of short-term hypoxic stress (0.1% O2, 24 h) on adipose tissue-derived MSC motility at tissue-related O2 level. No significant changes in integrin expression were detected after short-term hypoxic stress. However, O2 deprivation provoked vimentin disassembly and actin polymerisation and increased cell stiffness. In addition, hypoxic stress induced the downregulation of ACTR3, DSTN, MACF1, MID1, MYPT1, NCK1, ROCK1, TIAM1, and WASF1 expression, the products of which are known to be involved in leading edge formation and cell translocation. These changes were accompanied by the attenuation of targeted and nontargeted migration of MSCs after short-term hypoxic exposure, as demonstrated in scratch and transwell migration assays. These results indicate that acute hypoxic stress can modulate MSC function in their native milieu, preventing their mobilisation from sites of injury. |
| format | Article |
| id | doaj-art-7f2de871bf09412983ee697ad1f69c38 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-7f2de871bf09412983ee697ad1f69c382025-08-20T03:35:40ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/72605627260562Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal CellsOlga O. Udartseva0Margarita V. Lobanova1Elena R. Andreeva2Sergey V. Buravkov3Irina V. Ogneva4Ludmila B. Buravkova5Institute of Biomedical Problems, Russian Academy of Science, Khoroshevskoe Shosse 76A, Moscow 123007, RussiaInstitute of Biomedical Problems, Russian Academy of Science, Khoroshevskoe Shosse 76A, Moscow 123007, RussiaInstitute of Biomedical Problems, Russian Academy of Science, Khoroshevskoe Shosse 76A, Moscow 123007, RussiaLomonosov Moscow State University, Faculty of Fundamental Medicine, Lomonosovsky Prospect 31-5, Moscow 117192, RussiaInstitute of Biomedical Problems, Russian Academy of Science, Khoroshevskoe Shosse 76A, Moscow 123007, RussiaInstitute of Biomedical Problems, Russian Academy of Science, Khoroshevskoe Shosse 76A, Moscow 123007, RussiaThe ability of mesenchymal stromal (stem) cells (MSCs) to be mobilised from their local depot towards sites of injury and to participate in tissue repair makes these cells promising candidates for cell therapy. Physiological O2 tension in an MSC niche in vivo is about 4–7%. However, most in vitro studies of MSC functional activity are performed at 20% O2. Therefore, this study focused on the effects of short-term hypoxic stress (0.1% O2, 24 h) on adipose tissue-derived MSC motility at tissue-related O2 level. No significant changes in integrin expression were detected after short-term hypoxic stress. However, O2 deprivation provoked vimentin disassembly and actin polymerisation and increased cell stiffness. In addition, hypoxic stress induced the downregulation of ACTR3, DSTN, MACF1, MID1, MYPT1, NCK1, ROCK1, TIAM1, and WASF1 expression, the products of which are known to be involved in leading edge formation and cell translocation. These changes were accompanied by the attenuation of targeted and nontargeted migration of MSCs after short-term hypoxic exposure, as demonstrated in scratch and transwell migration assays. These results indicate that acute hypoxic stress can modulate MSC function in their native milieu, preventing their mobilisation from sites of injury.http://dx.doi.org/10.1155/2016/7260562 |
| spellingShingle | Olga O. Udartseva Margarita V. Lobanova Elena R. Andreeva Sergey V. Buravkov Irina V. Ogneva Ludmila B. Buravkova Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells Stem Cells International |
| title | Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells |
| title_full | Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells |
| title_fullStr | Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells |
| title_full_unstemmed | Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells |
| title_short | Acute Hypoxic Stress Affects Migration Machinery of Tissue O2-Adapted Adipose Stromal Cells |
| title_sort | acute hypoxic stress affects migration machinery of tissue o2 adapted adipose stromal cells |
| url | http://dx.doi.org/10.1155/2016/7260562 |
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