Phenotypic Detection of Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Uropathogens in Sokoto Metropolis

Extended spectrum beta-lactamases (ESBL) produced by Gram-negative uropathogens constitute an emerging public-health concern. The aim of the study was to determine the prevalence of ESBL producing enterobacteriaceae uropathogens in Sokoto. The study was undertaken at Specialist Hospital Sokoto (SHS)...

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Main Author: Nuhu Tanko *, Chado Jibril Baba, Olowookere Ahmed, Hussain Ungokore Yahaya, Nura Umar Muhammad
Format: Article
Language:English
Published: Hammer Head Production Limited 2017-06-01
Series:Sokoto Journal of Medical Laboratory Science
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Online Access:https://sokjmls.com.ng/index.php/SJMLS/article/view/346
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author Nuhu Tanko *, Chado Jibril Baba, Olowookere Ahmed, Hussain Ungokore Yahaya, Nura Umar Muhammad
author_facet Nuhu Tanko *, Chado Jibril Baba, Olowookere Ahmed, Hussain Ungokore Yahaya, Nura Umar Muhammad
author_sort Nuhu Tanko *, Chado Jibril Baba, Olowookere Ahmed, Hussain Ungokore Yahaya, Nura Umar Muhammad
collection DOAJ
description Extended spectrum beta-lactamases (ESBL) produced by Gram-negative uropathogens constitute an emerging public-health concern. The aim of the study was to determine the prevalence of ESBL producing enterobacteriaceae uropathogens in Sokoto. The study was undertaken at Specialist Hospital Sokoto (SHS) over a 2-month period. Ninety-one urine samples were collected. The isolates collected were identified using biochemical tests. All isolates were tested for susceptibility to eight different antibiotics by the modified Kirby Baeur disc diffusion. Screening and confirmatory tests for ESBL production were done according to Clinical Laboratory Standard Institute (CLSI) guidelines. A total of 25 isolates were Gram-negative which accounted for 41% prevalence, while 59% accounted for Gram-positive. E. coli was the most common isolates (44%), followed by Proteus mirabilis (20%), Klebsiella spp (16%), Enterobacter spp (12%), and Salmonella spp (8%).  All isolates displayed multi-drug resistance. The overall production of ESBL-producing isolates was 16%. Our study confirms the prevalence of ESBL-producing uropathogens in Sokoto.
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institution Kabale University
issn 2536-7153
language English
publishDate 2017-06-01
publisher Hammer Head Production Limited
record_format Article
series Sokoto Journal of Medical Laboratory Science
spelling doaj-art-7f196bfba0f94bac8b6160cae3dc6a522025-08-20T03:41:46ZengHammer Head Production LimitedSokoto Journal of Medical Laboratory Science2536-71532017-06-0122346Phenotypic Detection of Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Uropathogens in Sokoto MetropolisNuhu Tanko *, Chado Jibril Baba, Olowookere Ahmed, Hussain Ungokore Yahaya, Nura Umar MuhammadExtended spectrum beta-lactamases (ESBL) produced by Gram-negative uropathogens constitute an emerging public-health concern. The aim of the study was to determine the prevalence of ESBL producing enterobacteriaceae uropathogens in Sokoto. The study was undertaken at Specialist Hospital Sokoto (SHS) over a 2-month period. Ninety-one urine samples were collected. The isolates collected were identified using biochemical tests. All isolates were tested for susceptibility to eight different antibiotics by the modified Kirby Baeur disc diffusion. Screening and confirmatory tests for ESBL production were done according to Clinical Laboratory Standard Institute (CLSI) guidelines. A total of 25 isolates were Gram-negative which accounted for 41% prevalence, while 59% accounted for Gram-positive. E. coli was the most common isolates (44%), followed by Proteus mirabilis (20%), Klebsiella spp (16%), Enterobacter spp (12%), and Salmonella spp (8%).  All isolates displayed multi-drug resistance. The overall production of ESBL-producing isolates was 16%. Our study confirms the prevalence of ESBL-producing uropathogens in Sokoto.https://sokjmls.com.ng/index.php/SJMLS/article/view/346phenotypic detection, esbl, uropathogens
spellingShingle Nuhu Tanko *, Chado Jibril Baba, Olowookere Ahmed, Hussain Ungokore Yahaya, Nura Umar Muhammad
Phenotypic Detection of Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Uropathogens in Sokoto Metropolis
Sokoto Journal of Medical Laboratory Science
phenotypic detection, esbl, uropathogens
title Phenotypic Detection of Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Uropathogens in Sokoto Metropolis
title_full Phenotypic Detection of Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Uropathogens in Sokoto Metropolis
title_fullStr Phenotypic Detection of Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Uropathogens in Sokoto Metropolis
title_full_unstemmed Phenotypic Detection of Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Uropathogens in Sokoto Metropolis
title_short Phenotypic Detection of Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Uropathogens in Sokoto Metropolis
title_sort phenotypic detection of extended spectrum beta lactamase esbl produced by gram negative uropathogens in sokoto metropolis
topic phenotypic detection, esbl, uropathogens
url https://sokjmls.com.ng/index.php/SJMLS/article/view/346
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