Hylotelephium mingjinianum Extract Attenuates Oxidative Stress and Inflammation in Experimental Periodontitis
Lin Yuan,1 Chenfei Kong,2 Naixu Shi,1 Jiapeng Chen,3 Tianfu Zhang,1 Xiaofeng Wang1 1Department of Stomatology, China-Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 2Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, People’s Repub...
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Dove Medical Press
2025-06-01
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| author | Yuan L Kong C Shi N Chen J Zhang T Wang X |
| author_facet | Yuan L Kong C Shi N Chen J Zhang T Wang X |
| author_sort | Yuan L |
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| description | Lin Yuan,1 Chenfei Kong,2 Naixu Shi,1 Jiapeng Chen,3 Tianfu Zhang,1 Xiaofeng Wang1 1Department of Stomatology, China-Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 2Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 3Oral and Maxillofacial Surgery, Changchun Stomatological Hospital, Changchun, People’s Republic of ChinaCorrespondence: Xiaofeng Wang, Department of Stomatology, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, Jilin, 130033, People’s Republic of China, Tel +86-13756255115, Email wangxiaofeng@jlu.edu.cnBackground: Hylotelephium mingjinianum is a traditional Chinese herbal medicine with anti-inflammatory, analgesic and antibacterial effects. However, its role in the treatment of Periodontal disease has not been elaborated in detail.Purpose: This study aimed to elucidate the dual-target anti-inflammatory and antioxidant mechanisms of Hylotelephium mingjinianum extract (HME) in periodontitis treatment, focusing on its modulation of the Nrf2/NF-κB crosstalk.Methods: The periodontitis animal model of SD rats was established by ligation combined with Porphyromonas gingivalis (Pg) stimulation. The rats were locally administered HME (0.25%, 0.5%, and 1%, 0.5 mL/twice/day) for 14 days. Inflammatory responses of alveolar bone, expression of osteogenic related biomarkers, and activation of Nrf2/NF-κB signaling pathway were detected. In addition, LPS induced human periodontal ligament cells (HPDLs) to measure the effect of HME on cell viability, inflammatory response, Nrf2/NF- κB pathway and oxidative stress.Results: HME administration demonstrated significant efficacy in a ligature-induced periodontitis rat model: serum pro-inflammatory cytokines (IL-1β, IL-2, IL-6, IL-18, GM-CSF, and ICAM1) decreased by 24.9– 50.6% at high HME concentrations, while Th2-related factors IL-4/IL-13 returned to baseline levels. Histopathological analysis revealed that HME maintained gingival epithelial integrity and suppressed osteoclast activity in a dose-dependent manner by downregulating RANKL. Mechanistic studies indicated that HME attenuated NF-κB activation by reducing nuclear p65 protein (44.1%) and enhanced the Nrf2-mediated antioxidant response, normalizing oxidative stress markers (MDA decreased by 55.3%; SOD restored to 142.3 U/mg). In vitro experiments confirmed HME’s cytocompatibility at concentrations below 200μg/mL and its resistance to LPS stimulation, reducing ROS overaccumulation by 16.46% through modulation of the Nrf2/NF-κB axis.Conclusion: HME inhibits the progression of periodontitis in rats by downregulating the expression of inflammatory factors, alleviating oxidative stress, and repairing the Nrf2/NF-κB signaling pathway. Keywords: periodontitis, inflammatory factors, RANKL, Nrf2, NF-κB signaling pathway, ROS |
| format | Article |
| id | doaj-art-7f0933963783433c8c046819d3aabcc2 |
| institution | Kabale University |
| issn | 1178-7031 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Dove Medical Press |
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| series | Journal of Inflammation Research |
| spelling | doaj-art-7f0933963783433c8c046819d3aabcc22025-08-20T03:26:57ZengDove Medical PressJournal of Inflammation Research1178-70312025-06-01Volume 18Issue 182638276104116Hylotelephium mingjinianum Extract Attenuates Oxidative Stress and Inflammation in Experimental PeriodontitisYuan L0Kong C1Shi NChen J2Zhang T3Wang X4Departments of StomatologyScientific Research CenterOral and Maxillofacial SurgeryDepartment of StomatologyDepartments of StomatologyLin Yuan,1 Chenfei Kong,2 Naixu Shi,1 Jiapeng Chen,3 Tianfu Zhang,1 Xiaofeng Wang1 1Department of Stomatology, China-Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 2Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 3Oral and Maxillofacial Surgery, Changchun Stomatological Hospital, Changchun, People’s Republic of ChinaCorrespondence: Xiaofeng Wang, Department of Stomatology, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, Jilin, 130033, People’s Republic of China, Tel +86-13756255115, Email wangxiaofeng@jlu.edu.cnBackground: Hylotelephium mingjinianum is a traditional Chinese herbal medicine with anti-inflammatory, analgesic and antibacterial effects. However, its role in the treatment of Periodontal disease has not been elaborated in detail.Purpose: This study aimed to elucidate the dual-target anti-inflammatory and antioxidant mechanisms of Hylotelephium mingjinianum extract (HME) in periodontitis treatment, focusing on its modulation of the Nrf2/NF-κB crosstalk.Methods: The periodontitis animal model of SD rats was established by ligation combined with Porphyromonas gingivalis (Pg) stimulation. The rats were locally administered HME (0.25%, 0.5%, and 1%, 0.5 mL/twice/day) for 14 days. Inflammatory responses of alveolar bone, expression of osteogenic related biomarkers, and activation of Nrf2/NF-κB signaling pathway were detected. In addition, LPS induced human periodontal ligament cells (HPDLs) to measure the effect of HME on cell viability, inflammatory response, Nrf2/NF- κB pathway and oxidative stress.Results: HME administration demonstrated significant efficacy in a ligature-induced periodontitis rat model: serum pro-inflammatory cytokines (IL-1β, IL-2, IL-6, IL-18, GM-CSF, and ICAM1) decreased by 24.9– 50.6% at high HME concentrations, while Th2-related factors IL-4/IL-13 returned to baseline levels. Histopathological analysis revealed that HME maintained gingival epithelial integrity and suppressed osteoclast activity in a dose-dependent manner by downregulating RANKL. Mechanistic studies indicated that HME attenuated NF-κB activation by reducing nuclear p65 protein (44.1%) and enhanced the Nrf2-mediated antioxidant response, normalizing oxidative stress markers (MDA decreased by 55.3%; SOD restored to 142.3 U/mg). In vitro experiments confirmed HME’s cytocompatibility at concentrations below 200μg/mL and its resistance to LPS stimulation, reducing ROS overaccumulation by 16.46% through modulation of the Nrf2/NF-κB axis.Conclusion: HME inhibits the progression of periodontitis in rats by downregulating the expression of inflammatory factors, alleviating oxidative stress, and repairing the Nrf2/NF-κB signaling pathway. Keywords: periodontitis, inflammatory factors, RANKL, Nrf2, NF-κB signaling pathway, ROShttps://www.dovepress.com/hylotelephium-mingjinianum-extract-attenuates-oxidative-stress-and-inf-peer-reviewed-fulltext-article-JIRPeriodontitisinflammatory factorsRANKLNrf2NF-κB signaling pathwayROS |
| spellingShingle | Yuan L Kong C Shi N Chen J Zhang T Wang X Hylotelephium mingjinianum Extract Attenuates Oxidative Stress and Inflammation in Experimental Periodontitis Journal of Inflammation Research Periodontitis inflammatory factors RANKL Nrf2 NF-κB signaling pathway ROS |
| title | Hylotelephium mingjinianum Extract Attenuates Oxidative Stress and Inflammation in Experimental Periodontitis |
| title_full | Hylotelephium mingjinianum Extract Attenuates Oxidative Stress and Inflammation in Experimental Periodontitis |
| title_fullStr | Hylotelephium mingjinianum Extract Attenuates Oxidative Stress and Inflammation in Experimental Periodontitis |
| title_full_unstemmed | Hylotelephium mingjinianum Extract Attenuates Oxidative Stress and Inflammation in Experimental Periodontitis |
| title_short | Hylotelephium mingjinianum Extract Attenuates Oxidative Stress and Inflammation in Experimental Periodontitis |
| title_sort | hylotelephium mingjinianum extract attenuates oxidative stress and inflammation in experimental periodontitis |
| topic | Periodontitis inflammatory factors RANKL Nrf2 NF-κB signaling pathway ROS |
| url | https://www.dovepress.com/hylotelephium-mingjinianum-extract-attenuates-oxidative-stress-and-inf-peer-reviewed-fulltext-article-JIR |
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