Valorisation of Winery By-Products: Revealing the Polyphenolic Profile of Grape Stems and Their Inhibitory Effects on Skin Aging-Enzymes for Cosmetic and Pharmaceutical Applications
Grape (<i>Vitis vinifera</i> L.) stems, a by-product of winemaking, possess significant potential value due to their rich polyphenolic composition, which allows their exploitation for cosmetic and pharmaceutical applications. This presents a promising opportunity for valorisation aimed a...
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MDPI AG
2024-11-01
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| author | Rui Dias-Costa Concepción Medrano-Padial Raquel Fernandes Raúl Domínguez-Perles Irene Gouvinhas Ana Novo Barros |
| author_facet | Rui Dias-Costa Concepción Medrano-Padial Raquel Fernandes Raúl Domínguez-Perles Irene Gouvinhas Ana Novo Barros |
| author_sort | Rui Dias-Costa |
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| description | Grape (<i>Vitis vinifera</i> L.) stems, a by-product of winemaking, possess significant potential value due to their rich polyphenolic composition, which allows their exploitation for cosmetic and pharmaceutical applications. This presents a promising opportunity for valorisation aimed at developing innovative products with potential health-promoting effects. In this study, the polyphenolic profile of extracts from grape stems of seven white grape varieties was determined using spectrophotometric and chromatographic methods, specifically high-performance liquid chromatography coupled with a photodiode array detector and electrospray ionization multi-stage mass spectrometry (HPLC-PDA-ESI-MSn), as well as on their ferric-reducing antioxidant power (FRAP) and radical scavenging capacity, using 2,2-diphenyl-1-picrylhydrazyl (DPPH<sup>●</sup>) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS<sup>●+</sup>) radicals. This study also evaluated the anti-aging activity and skin depigmenting activity of these extracts. These findings revealed a diverse polyphenolic profile, encompassing proanthocyanidins and catechin derivatives (PCDs), phenolic acids, and flavonols. Among the varieties studied, ‘Códega do Larinho’ exhibited the highest concentrations of six distinct polyphenols and the highest total phenolic content. It also demonstrated the highest results for antioxidant capacity and elastase and tyrosinase inhibition. Pearson’s correlation analysis showed a significant positive correlation between certain PCDs with both FRAP and DPPH assays, as well as between the identified flavonols and anti-elastase activity. These results underscore the potential health benefits of grape stem extracts and emphasize the importance of their polyphenolic composition in enhancing antioxidant and anti-aging properties, thus supporting their application in different industries. |
| format | Article |
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| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-7f042a96fd544bdeb1dc26b4fcd171222025-08-20T01:53:54ZengMDPI AGMolecules1420-30492024-11-012922543710.3390/molecules29225437Valorisation of Winery By-Products: Revealing the Polyphenolic Profile of Grape Stems and Their Inhibitory Effects on Skin Aging-Enzymes for Cosmetic and Pharmaceutical ApplicationsRui Dias-Costa0Concepción Medrano-Padial1Raquel Fernandes2Raúl Domínguez-Perles3Irene Gouvinhas4Ana Novo Barros5Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, PortugalLaboratorio de Fitoquímica y Alimentos Saludables (LabFAS), Centro de Edafología y Biología Aplicada del Segura-Spanish Council for Scientific Research (EBAS-CSIC), University Campus of Espinardo 25, 30100 Murcia, SpainCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, PortugalLaboratorio de Fitoquímica y Alimentos Saludables (LabFAS), Centro de Edafología y Biología Aplicada del Segura-Spanish Council for Scientific Research (EBAS-CSIC), University Campus of Espinardo 25, 30100 Murcia, SpainCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, PortugalGrape (<i>Vitis vinifera</i> L.) stems, a by-product of winemaking, possess significant potential value due to their rich polyphenolic composition, which allows their exploitation for cosmetic and pharmaceutical applications. This presents a promising opportunity for valorisation aimed at developing innovative products with potential health-promoting effects. In this study, the polyphenolic profile of extracts from grape stems of seven white grape varieties was determined using spectrophotometric and chromatographic methods, specifically high-performance liquid chromatography coupled with a photodiode array detector and electrospray ionization multi-stage mass spectrometry (HPLC-PDA-ESI-MSn), as well as on their ferric-reducing antioxidant power (FRAP) and radical scavenging capacity, using 2,2-diphenyl-1-picrylhydrazyl (DPPH<sup>●</sup>) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS<sup>●+</sup>) radicals. This study also evaluated the anti-aging activity and skin depigmenting activity of these extracts. These findings revealed a diverse polyphenolic profile, encompassing proanthocyanidins and catechin derivatives (PCDs), phenolic acids, and flavonols. Among the varieties studied, ‘Códega do Larinho’ exhibited the highest concentrations of six distinct polyphenols and the highest total phenolic content. It also demonstrated the highest results for antioxidant capacity and elastase and tyrosinase inhibition. Pearson’s correlation analysis showed a significant positive correlation between certain PCDs with both FRAP and DPPH assays, as well as between the identified flavonols and anti-elastase activity. These results underscore the potential health benefits of grape stem extracts and emphasize the importance of their polyphenolic composition in enhancing antioxidant and anti-aging properties, thus supporting their application in different industries.https://www.mdpi.com/1420-3049/29/22/5437grape stemspolyphenolic profileantioxidant capacityanti-agingvalorisation |
| spellingShingle | Rui Dias-Costa Concepción Medrano-Padial Raquel Fernandes Raúl Domínguez-Perles Irene Gouvinhas Ana Novo Barros Valorisation of Winery By-Products: Revealing the Polyphenolic Profile of Grape Stems and Their Inhibitory Effects on Skin Aging-Enzymes for Cosmetic and Pharmaceutical Applications Molecules grape stems polyphenolic profile antioxidant capacity anti-aging valorisation |
| title | Valorisation of Winery By-Products: Revealing the Polyphenolic Profile of Grape Stems and Their Inhibitory Effects on Skin Aging-Enzymes for Cosmetic and Pharmaceutical Applications |
| title_full | Valorisation of Winery By-Products: Revealing the Polyphenolic Profile of Grape Stems and Their Inhibitory Effects on Skin Aging-Enzymes for Cosmetic and Pharmaceutical Applications |
| title_fullStr | Valorisation of Winery By-Products: Revealing the Polyphenolic Profile of Grape Stems and Their Inhibitory Effects on Skin Aging-Enzymes for Cosmetic and Pharmaceutical Applications |
| title_full_unstemmed | Valorisation of Winery By-Products: Revealing the Polyphenolic Profile of Grape Stems and Their Inhibitory Effects on Skin Aging-Enzymes for Cosmetic and Pharmaceutical Applications |
| title_short | Valorisation of Winery By-Products: Revealing the Polyphenolic Profile of Grape Stems and Their Inhibitory Effects on Skin Aging-Enzymes for Cosmetic and Pharmaceutical Applications |
| title_sort | valorisation of winery by products revealing the polyphenolic profile of grape stems and their inhibitory effects on skin aging enzymes for cosmetic and pharmaceutical applications |
| topic | grape stems polyphenolic profile antioxidant capacity anti-aging valorisation |
| url | https://www.mdpi.com/1420-3049/29/22/5437 |
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