In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota.
In vitro gut modeling provides a useful platform for a fast and reproducible assessment of treatment-related changes. Currently, pig intestinal fermentation models are mainly batch models with important inherent limitations. In this study we developed a novel in vitro continuous fermentation model,...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094123&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850026559587483648 |
|---|---|
| author | Sabine A Tanner Annina Zihler Berner Eugenia Rigozzi Franck Grattepanche Christophe Chassard Christophe Lacroix |
| author_facet | Sabine A Tanner Annina Zihler Berner Eugenia Rigozzi Franck Grattepanche Christophe Chassard Christophe Lacroix |
| author_sort | Sabine A Tanner |
| collection | DOAJ |
| description | In vitro gut modeling provides a useful platform for a fast and reproducible assessment of treatment-related changes. Currently, pig intestinal fermentation models are mainly batch models with important inherent limitations. In this study we developed a novel in vitro continuous fermentation model, mimicking the porcine proximal colon, which we validated during 54 days of fermentation. This model, based on our recent PolyFermS design, allows comparing different treatment effects on the same microbiota. It is composed of a first-stage inoculum reactor seeded with immobilized fecal swine microbiota and used to constantly inoculate (10% v/v) five second-stage reactors, with all reactors fed with fresh nutritive chyme medium and set to mimic the swine proximal colon. Reactor effluents were analyzed for metabolite concentrations and bacterial composition by HPLC and quantitative PCR, and microbial diversity was assessed by 454 pyrosequencing. The novel PolyFermS featured stable microbial composition, diversity and metabolite production, consistent with bacterial activity reported for swine proximal colon in vivo. The constant inoculation provided by the inoculum reactor generated reproducible microbial ecosystems in all second-stage reactors, allowing the simultaneous investigation and direct comparison of different treatments on the same porcine gut microbiota. Our data demonstrate the unique features of this novel PolyFermS design for the swine proximal colon. The model provides a tool for efficient, reproducible and cost-effective screening of environmental factors, such as dietary additives, on pig colonic fermentation. |
| format | Article |
| id | doaj-art-7ef7c5149d9a463c9abb32b6a7873d73 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7ef7c5149d9a463c9abb32b6a7873d732025-08-20T03:00:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9412310.1371/journal.pone.0094123In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota.Sabine A TannerAnnina Zihler BernerEugenia RigozziFranck GrattepancheChristophe ChassardChristophe LacroixIn vitro gut modeling provides a useful platform for a fast and reproducible assessment of treatment-related changes. Currently, pig intestinal fermentation models are mainly batch models with important inherent limitations. In this study we developed a novel in vitro continuous fermentation model, mimicking the porcine proximal colon, which we validated during 54 days of fermentation. This model, based on our recent PolyFermS design, allows comparing different treatment effects on the same microbiota. It is composed of a first-stage inoculum reactor seeded with immobilized fecal swine microbiota and used to constantly inoculate (10% v/v) five second-stage reactors, with all reactors fed with fresh nutritive chyme medium and set to mimic the swine proximal colon. Reactor effluents were analyzed for metabolite concentrations and bacterial composition by HPLC and quantitative PCR, and microbial diversity was assessed by 454 pyrosequencing. The novel PolyFermS featured stable microbial composition, diversity and metabolite production, consistent with bacterial activity reported for swine proximal colon in vivo. The constant inoculation provided by the inoculum reactor generated reproducible microbial ecosystems in all second-stage reactors, allowing the simultaneous investigation and direct comparison of different treatments on the same porcine gut microbiota. Our data demonstrate the unique features of this novel PolyFermS design for the swine proximal colon. The model provides a tool for efficient, reproducible and cost-effective screening of environmental factors, such as dietary additives, on pig colonic fermentation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094123&type=printable |
| spellingShingle | Sabine A Tanner Annina Zihler Berner Eugenia Rigozzi Franck Grattepanche Christophe Chassard Christophe Lacroix In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota. PLoS ONE |
| title | In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota. |
| title_full | In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota. |
| title_fullStr | In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota. |
| title_full_unstemmed | In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota. |
| title_short | In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota. |
| title_sort | in vitro continuous fermentation model polyferms of the swine proximal colon for simultaneous testing on the same gut microbiota |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094123&type=printable |
| work_keys_str_mv | AT sabineatanner invitrocontinuousfermentationmodelpolyfermsoftheswineproximalcolonforsimultaneoustestingonthesamegutmicrobiota AT anninazihlerberner invitrocontinuousfermentationmodelpolyfermsoftheswineproximalcolonforsimultaneoustestingonthesamegutmicrobiota AT eugeniarigozzi invitrocontinuousfermentationmodelpolyfermsoftheswineproximalcolonforsimultaneoustestingonthesamegutmicrobiota AT franckgrattepanche invitrocontinuousfermentationmodelpolyfermsoftheswineproximalcolonforsimultaneoustestingonthesamegutmicrobiota AT christophechassard invitrocontinuousfermentationmodelpolyfermsoftheswineproximalcolonforsimultaneoustestingonthesamegutmicrobiota AT christophelacroix invitrocontinuousfermentationmodelpolyfermsoftheswineproximalcolonforsimultaneoustestingonthesamegutmicrobiota |