The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function.
Shigella species cause bacillary dysentery, especially among young individuals. Shigellae target the human colon for invasion; however, the initial adhesion mechanism is poorly understood. The Shigella surface protein IcsA, in addition to its role in actin-based motility, acts as a host cell adhesin...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227425&type=printable |
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| author | Jilong Qin Matthew Thomas Doyle Elizabeth Ngoc Hoa Tran Renato Morona |
| author_facet | Jilong Qin Matthew Thomas Doyle Elizabeth Ngoc Hoa Tran Renato Morona |
| author_sort | Jilong Qin |
| collection | DOAJ |
| description | Shigella species cause bacillary dysentery, especially among young individuals. Shigellae target the human colon for invasion; however, the initial adhesion mechanism is poorly understood. The Shigella surface protein IcsA, in addition to its role in actin-based motility, acts as a host cell adhesin through unknown mechanism(s). Here we confirmed the role of IcsA in cell adhesion and defined the region required for IcsA adhesin activity. Purified IcsA passenger domain was able block S. flexneri adherence and was also used as a molecular probe that recognised multiple components from host cells. The region within IcsA's functional passenger domain (aa 138-148) was identified by mutagenesis. Upon the deletion of this region, the purified IcsAΔ138-148 was found to no longer block S. flexneri adherence and had reduced ability to interact with host molecules. Furthermore, S. flexneri expressing IcsAΔ138-148 was found to be significantly defective in both cell adherence and invasion. Taken together, our data identify an adherence region within the IcsA functional domain and provides useful information for designing therapeutics for Shigella infection. |
| format | Article |
| id | doaj-art-7ef1192a4fa54660977433529c3b5bc9 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7ef1192a4fa54660977433529c3b5bc92025-08-20T02:17:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01151e022742510.1371/journal.pone.0227425The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function.Jilong QinMatthew Thomas DoyleElizabeth Ngoc Hoa TranRenato MoronaShigella species cause bacillary dysentery, especially among young individuals. Shigellae target the human colon for invasion; however, the initial adhesion mechanism is poorly understood. The Shigella surface protein IcsA, in addition to its role in actin-based motility, acts as a host cell adhesin through unknown mechanism(s). Here we confirmed the role of IcsA in cell adhesion and defined the region required for IcsA adhesin activity. Purified IcsA passenger domain was able block S. flexneri adherence and was also used as a molecular probe that recognised multiple components from host cells. The region within IcsA's functional passenger domain (aa 138-148) was identified by mutagenesis. Upon the deletion of this region, the purified IcsAΔ138-148 was found to no longer block S. flexneri adherence and had reduced ability to interact with host molecules. Furthermore, S. flexneri expressing IcsAΔ138-148 was found to be significantly defective in both cell adherence and invasion. Taken together, our data identify an adherence region within the IcsA functional domain and provides useful information for designing therapeutics for Shigella infection.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227425&type=printable |
| spellingShingle | Jilong Qin Matthew Thomas Doyle Elizabeth Ngoc Hoa Tran Renato Morona The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function. PLoS ONE |
| title | The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function. |
| title_full | The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function. |
| title_fullStr | The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function. |
| title_full_unstemmed | The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function. |
| title_short | The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function. |
| title_sort | virulence domain of shigella icsa contains a subregion with specific host cell adhesion function |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227425&type=printable |
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